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Interaction between bovine serum albumin and Indo-1 using fluorescence spectroscopic method

  • Research Article
  • Published:
Frontiers of Chemistry in China

Abstract

This work attempts to calculate the binding-site number using fluorescence spectroscopic method with bovine serum albumin (BSA) and Indo-1 as protein and ligand models, respectively. The method for calculating the binding-site number in BSA for Indo-1 was developed based on the relationships between changes in Indo-1 fluorescence intensity and the analytical concentration of BSA. The interaction between BSA with Indo-1 was investigated comprehensively using fluorescence techniques as well as fluorescence resonance energy transfer, and the thermodynamic parameters were calculated according to the effect of enthalpy on temperature. Three binding sites in BSA for Indo-1 were revealed, and the distances from Trp212 in BSA to the three binding sites were 2.93, 2.57 and 2.40 nm, respectively. It was also proven that Indo-1 embedded into the three hydrophobic cavities of BSA by hydrophobic association. This paper provides a reference on calculating the binding-site number in proteins for ligands and studying their interactions by fluorescence spectroscopic methods. In fluorescent quenching experiments, fluorescence changes were automatically recorded in real time by combining the Microlab 500 Series Dispenser and PTI fluorescence apparatus.

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Authors and Affiliations

  1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China

    Haixin Bai, Cheng Yang & Xiurong Yang

  2. Graduate University of Chinese Academy of Sciences, Beijing, 100039, China

    Haixin Bai

Authors
  1. Haixin Bai
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  2. Cheng Yang
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  3. Xiurong Yang
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Correspondence to Xiurong Yang.

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Translated from Chemical Journal of Chinese Universities, 2007, 28(2): 227–233 [译自: 高等学校化学学报]

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Bai, H., Yang, C. & Yang, X. Interaction between bovine serum albumin and Indo-1 using fluorescence spectroscopic method. Front. Chem. China 3, 105–111 (2008). https://doi.org/10.1007/s11458-008-0013-4

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  • DOI: https://doi.org/10.1007/s11458-008-0013-4

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