Abstract
Thioredoxin is a redox protein while adiponectin is an adipokine. Their relationship has been less appreciated. Here we show that in serum of patients with acute exacerbations of chronic obstructive Pulmonary Disease, decreased activity of thioredoxin coexists with increased level of adiponectin as partial pressure of arterial oxygen decreases. From the results with adiponectin-knockout mice and in vitro experiments, we have found a reciprocal inhibitory relationship, by which adiponectin inhibits cytosolic thioredoxin (Trx1) expression, whereas serum Trx1 influences adiponectin multimerization. The association between extracellular Trx1 and adiponectin attenuates their functions. This relationship is dynamic, and correlated with a body’s physiological conditions.
摘要
氧化还原蛋白“硫氧还蛋白”和脂肪细胞因子“脂联素”间的关系鲜有报道。我们发现,慢性阻塞性肺疾病急性发作期(AECOPD)患者, 随着动脉血氧分压降低, 血清脂联素水平显著增高, 并伴随着血清硫氧还蛋白活性显著降低。通过分析脂联素基因敲除小鼠及体外细胞和分子水平上的机制探讨, 我们揭示了脂联素和胞质硫氧还蛋白(Trx1)间的相互抑制关系。脂联素能抑制Trx1表达,而血清Trx1可影响脂联素聚集。此外,脂联素和Trx1通过形成复合物, 影响彼此的生物学活性。这种分子间的动态调控关联着机体的生理状态。
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Acknowledgments
This work was supported by the National Natural Science Foundation of China (31170764), the Main Direction Program of Knowledge Innovation of Chinese Academy of Sciences (KSCX2-EW-J-29), and Science and Education Integration Project of University of Chinese Academy of Sciences (KJRH2015-008).
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SPECIAL TOPIC: Lipid metabolism and human metabolic disorder
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Liu, JS., Xu, JY., Huang, J. et al. A reciprocal inhibitory relationship between adiponectin and mammalian cytosolic thioredoxin. Sci. Bull. 61, 1513–1521 (2016). https://doi.org/10.1007/s11434-016-1127-6
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DOI: https://doi.org/10.1007/s11434-016-1127-6