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Extracellular ATP mediates H2S-regulated stomatal movements and guard cell K+ current in a H2O2-dependent manner in Arabidopsis

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  • Life & Medical Sciences
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Science Bulletin

Abstract

Hydrogen sulfide (H2S) is a newly discovered gaseous signaling molecule and involved in ethylene and ABA-induced stomatal closure. As an important factor, extracellular ATP (eATP) was believed to participate in regulation of stomatal closing. However, the mechanism by which eATP mediates H2S-regulated stomatal closure remains unclear. Here, we employed Arabidopsis wild-type and mutant lines of ATP-binding cassette transporters (Atmrp4, Atmrp5 and their double mutant Atmrp4/5) to study the function of eATP in H2S-regulated stomatal movement. Our results indicated that H2S affected stomatal closing through stimulating guard cell outward K+ current. Moreover, we found that H2S induced eATP generation by regulating the activity of an ABC transporter. The inhibitor of ABC transporters, glibenclamide (Gli), could impair H2S-regulated stomatal closure and reduce H2S-dependent eATP accumulation in Atmrp4 and Atmrp5 mutants. In addition, the promotion effect of H2S on outward K+ currents was diminished in Atmrp4/5 double mutant. Our data suggested that hydrogen peroxide (H2O2) is required for H2S-induced stomatal closure, and the production of H2O2 is regulated by eATP via NADPH oxidase. Based on this work, we conclude that H2S-induced stomatal closure requires ABC transporter-dependent eATP production and subsequent NADPH oxidase-dependent H2O2 accumulation.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (31170237), the National Key Laboratory Program of China Agricultural University (SKLPPBKF11001) and Shandong Taishan Scholar Program. We thank Prof. Dong Chunhai and Dr. Qian Ma (Qingdao Agricultural University) for helpful suggestions and critical reading and polishing of the manuscript.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Shandong Key Laboratory of Plant Biotechnology in University, College of Life Sciences, Qingdao Agricultural University, Qingdao, 266109, China

    Lanxiang Wang, Yongmei Che, Lixia Hou & Xin Liu

  2. Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, College of Life Sciences, Shandong University, Jinan, 250100, China

    Xiaoyan Ma & Wei Zhang

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  1. Lanxiang Wang
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  2. Xiaoyan Ma
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  3. Yongmei Che
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  4. Lixia Hou
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  5. Xin Liu
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  6. Wei Zhang
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Correspondence to Xin Liu.

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Wang, L., Ma, X., Che, Y. et al. Extracellular ATP mediates H2S-regulated stomatal movements and guard cell K+ current in a H2O2-dependent manner in Arabidopsis . Sci. Bull. 60, 419–427 (2015). https://doi.org/10.1007/s11434-014-0659-x

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  • DOI: https://doi.org/10.1007/s11434-014-0659-x

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