Abstract
A new generation of cement, microbe cement, has been developed in response to the ever increasing awareness of environmental protection. Microbe cement is a new strengthening material based on microbiologically induced precipitation of calcium carbonate. This paper confirms the feasibility of binding loose sand particles using microbe cement and details the cementation mechanism of microbe cement. We have also prepared microbe cementitious materials (biosandstones). The compressive strength of the bio-sandstone, which depends on the content of calcium carbonate produced by microbially induced precipitation and the bio-mediated calcite crystal size, could be up to 12 MPa at an age of 20 days. By increasing the precipitated calcite content, the compressive strength and microstructure of bio-sandstone could be improved.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Le Metayer-Levrel G, Castanier S, Orial G, et al. Applications of bacterial carbonatogenesis to the protection and regeneration of limestones in buildings and historic patrimony. Sediment Geol, 1999, 126: 25–34
Nemati M, Voordouw G. Modification of porous media permeability, using calcium carbonate produced enzymatically in situ. Enzyme Microb Technol, 2003, 33: 635–642
DeJong J T, Fritzges M B, Nusslein K. Microbially induced cementation to control sand response to undrained shear. J Geotech Geoenviron Eng, 2006, 132: 1381–1392
De Jong J T, Brina M M, Brian C M, et al. Bio-mediated soil improvement. Ecolo Eng, 2010, 36: 197–210
Whiffin V S. Microbial CaCO3 precipitation for the production of Biocement. Dissertation for Doctoral Degree. Western Australia: Murdoch University, 2004
Whiffin V S, Van Paassen L A, Harkes M P. Microbial carbonate precipitation as a soil improvement technique. Geomicrobiol J, 2007, 24: 417–423
Ivanov V, Chu J. Applications of microorganisms to geotechnical engineering for bioclogging and biocementation of soil in situ. Rev Environ Sci Biotechnol, 2008, 7: 139–153
De Muynck W, De Belie N, Verstraete W. Microbial carbonate precipitation in construction materials: A review. Ecol Eng, 2010, 36: 118–136
De Muynck W, Verbeken K, De Belie N, et al. Influence of urea and calcium dosage on the effectiveness of bacterially induced carbonate precipitation on limestone. Ecol Eng, 2010, 36: 99–111
De Muynck W, Debrouwer D, De Belie N, et al. Bacterial carbonate precipitation improves the durability of cementitious materials. Cem Concr Res, 2008, 38: 1005–1014
Qian C X, Wang J Y, Wang R X, et al. Corrosion protection of cement-based building materials by surface deposition of CaCO3 by Bacillus pasteurii. Mater Sci Eng C, 2009, 29: 1273–1280
Ramachandran S K, Ramakrishnan V, Bang S S. Remediation of concrete using micro-organisms. ACI Mater J, 2001, 1: 3–9
Ferris F G, Setehmeir L G. Bacteriogenic mineral plugging. United States Patent, 664769, 1992
Nemati M, Greene E A, Voordouw G. Permeability profile modification using bacterially formed calcium carbonate: Comparison with enzymic option. Process Biochem, 2005, 40: 925–933
Ferris F G, Phoenix V, Fujita Y, et al. Kinetics of calcite precipitation induced by ureolytic bacteria at 10°C to 20°C in artificial groundwater. Geochem Cosmochim Acta, 2003, 67: 1701–1722
Fujita Y, Ferris F G, Lawson R D, et al. Calcium carbonate precipitation by ureolytic subsurface bacteria. Geomicrobiol J, 2000, 17: 305–318
Warren L A, Maurice P A, Parmar N, et al. Microbially mediated calcium carbonate precipitation: Implications for interpreting calcite pecipitation and for solid-phase capture of inorganic contaminants. Geomicrobiol J, 2001, 18: 93–115
Chaturvedi S, Chandra R, Rai V. Isolation and characterization of Phragmites australis (L) rhizosphere bacteria from contaminated site for bioremediation of colored distillery effluent. Ecol Eng, 2006, 27: 202–207
Simon M A, Bonner J S, Page C A, et al. Evaluation of two commercial bioaugmentation products for enhanced removal of petroleum from a wetland. Ecol Eng, 2004, 22: 263–277
Bachmeier K L, Williams A E, Warmington J R, et al. Urease activity in microbiologically-induced calcite precipitation. J Biotechnol, 2002, 93: 171–181
Ferris F G, Stechmeier L G, Kantzas A, et al. Bacteriogenic mineral plugging. J Can Pet Technol, 1996, 35: 56–61
Ramakrishnan V, Ramesh K P, Bang S S. Bacterial concrete. In: Proc of SPIE-Int Society of Optical Engineering, 2001, 4234: 168–176
Morse J W. The kinetics of calcium carbonate dissolution and precipitation. In: Reeder R J, ed. Carbonates: Mineralogy and Chemistry, vol. 11. Washington, DC: Mineralogic Society of America, 1983. 227–264
Kantzas A, Stehmeier L, Marentette D F, et al. A novel method of sand consolidation through bacteriogenic mineral plugging. In: Jha K N, Maurits F M, eds. Proceedings of Annual Technical Meeting, 1992 Jun 7–10 Canada, Calgary: Society of Petroleum Engineers, 1992. 46
Qian C X, Pan Q F, Wang R X. Cementation of sand grains based on carbonate precipitation induced by microorganism. Sci China Tech Sci, 2010, 53: 2198–2206
Author information
Authors and Affiliations
Corresponding author
Additional information
This article is published with open access at Springerlink.com
Rights and permissions
This article is published under an open access license. Please check the 'Copyright Information' section either on this page or in the PDF for details of this license and what re-use is permitted. If your intended use exceeds what is permitted by the license or if you are unable to locate the licence and re-use information, please contact the Rights and Permissions team.
About this article
Cite this article
Rong, H., Qian, C. Characterization of microbe cementitious materials. Chin. Sci. Bull. 57, 1333–1338 (2012). https://doi.org/10.1007/s11434-012-5047-9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11434-012-5047-9