Abstract
A novel dual-label time-resolved fluoroimmunoassay method was developed for the simultaneous determination of chloramphenicol (CAP) and ractopamine (RAC) residues in 18 swine tissue samples, using anti-CAP and anti-RAC monoclonal antibodies labeled with europium (Eu3+) and samarium (Sm3+), respectively. The detection limits for CAP and RAC were 0.06 and 0.25 ng/g. The recovery from swine muscle samples was 102%–121% for CAP at spiking levels of 0.1–5 ng/g, and 69.8%–85.8% for RAC at spiking levels of 1–10 ng/g. The results obtained from the swine tissue samples using this method showed good agreement with those obtained using ELISA and GC-MS, with correlation coefficients (R) between 0.92–0.98.
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Holt D E, Bajoria R. The role of nitro-reduction and nitric oxide in the toxicity of chloramphenicol. Hum Exp Toxicol, 1999, 18: 111–118
Wareham D W, Wilson P. Chloramphenicol in the 21st century. Hosp Med, 2002, 63: 157–161
Posyniak A, Zmudzki J, Niedzielska J. Evaluation of sample preparation for control of chloramphenicol residues in porcine tissues by enzyme-linked immunosorbent assay and liquid chromatography. Anal Chim Acta, 2003, 483: 307–311
Masahiko T, Shigeki D, Taketoshi N. Determination of chloramphenicol residues in fish meats by liquid chromatography-atmospheric pressure photoionization mass spectrometry. J Chromatogr A, 2003, 1011: 67–75
Forti A F, Campana G, Simonella A, et al. Determination of chloramphenicol in honey by liquid chromatography-tandem mass spectrometry. Anal Chim Acta, 2005, 529: 257–263
Mottier P, Parisod V, Gremaud E, et al. Determination of the antibiotic chloramphenicol in meat and seafood products by liquid chromatography-electrospray ionization tandem mass spectrometry. J Chromatogr A, 2003, 994: 75–84
Raz S R, Bremer M G E G, Haasnoot W, et al. Label-free and multiplex detection of antibiotic residues in milk using imaging surface plasmon resonance-based immunosensor. Anal Chem, 2009, 81: 7743–7749
Yuan J, Oliver R, Aguilar M I, et al. Surface plasmon resonance assay for chloramphenicol. Anal Chem, 2008, 80: 8329–8333
Mohamed R, Richoz-Payot J, Gremaud E, et al. Advantages of molecularly imprinted polymers LC-ESI-MS/MS for the selective extraction and quantification of chloramphenicol in milk-based matrixes. Comparison with a classical sample preparation. Anal Chem, 2007, 79: 9557–9565
Gude T, Preiss A, Rubach K. Determination of chloramphenicol in muscle, liver, kidney and urine of pigs by means of immunoaffinity chromatography and gas chromatography with electron-capture detection. J Chromatogr B, 1995, 673: 197–204
Leach L M, Ellis M, Sutton D S, et al. The growth performance, carcass characteristics, and meat quality of halothane carrier and negative pigs. J Anim Sci, 74: 934-1996
Brambilla G, Cenci T, Franconi F, et al. Clinical and pharmacological profile in a clenbuterol epidemic poisoning of contaminated beef meat in Italy. Toxicol Lett, 2000, 114: 47–53
Kuiper H A, Noordam M Y, Van Dooren-Flipsen M M H, et al. Illegal use of beta-adrenergic agonists: European community. J Anim Sci, 1998, 76: 195–207
Antignac J P, Marchand P, Bizec L B, et al. Identification of ractopamine residues in tissue and urine samples at ultra-trace level using liquid chromatography-positive electrospray tandem mass spectrometry. J Chromatogr B, 2002, 774: 59–66
Shishani E, Chai S C, Jamokha S, et al. Determination of ractopamine in animal tissues by liquid chromatograghy-fluorescence and liquid chromatography/tandem mass spectrometry. Anal Chim Acta, 2003, 483: 137–145
Thompson C S, Haughey S A, Traynor I M, et al. Effective monitoring for ractopamine residues in samples of animal origin by SPR biosensor and mass spectrometry. Anal Chim Acta, 2008, 608: 217–225
Blanca J, Munoz P, Morgado M, et al. Determination of clenbuterol, ractopamine and zilpaterol in liver and urine by liquid chromatography tandem mass spectrometry. Anal Chim Acta, 2005, 529: 199–205
Haasnoot W, Bienenmann-Ploum M, Lamminmaki U, et al. Application of a multi-sulfonamide biosensor immunoassay for the detection of sulfadiazine and sulfamethoxazole residues in broiler serum and its use as a predictor of the levels in edible tissue. Anal Chim Acta, 2005, 552: 87–95
Lau J H W, Khoo C S, Murby J E. Determination of clenbuterol, salbutamol, and cimaterol in bovine retina by electrospray ionization-liquid chromatography-tandem mass spectrometry. J AOAC Int, 2004, 87: 31–38
Pfenning A P, Roybal J E, Rupp H S, et al. Simultaneous determination of residues of chloramphenicol, florfenicol, florfenicol amine, and thiamphenicol in shrimp tissue by gas chromatography with electron capture detection. J AOAC Int, 2000, 86: 26–30
Zhang H Y, Duan Z J, Wang L, et al. Hapten synthesis and development of polyclonal antibody-based multi-sulfonamide immunoassays. J Agr Food Chem, 2006, 54: 4499–4505
Haasnoot W, Bienenmann-Ploum M, Lamminmaki U, et al. Application of a multi-sulfonamide biosensor immunoassay for the detection of sulfadiazine and sulfamethoxazole residues in broiler serum and its use as a predictor of the levels in edible tissue. Anal Chim Acta, 2005, 552: 87–95
Shen J Z, Zhang Z, Yao Y, et al. Time-resolved fluoroimmunoassay for ractopamine in swine tissue. Anal Bioanal Chem, 2007, 387: 1561–1564
Shen J Z, Zhang Z, Yao Y, et al. A monoclonal antibody-based time-resolved fluoroimmunoassay for chloramphenicol in shrimp and chicken muscle. Anal Chim Acta, 2006, 575: 262–266
Kolosova A Y, Samsonova J V, Egorov A M. Competitive ELISA of chloraphenicol: Influence of immunoreagent structure and application of the method for the inspection of food of animal origin. Food Agr Immunnol, 2000, 12: 115–125
Wang J P, Zhang S X, Shen J Z. Development of immunoaffinity sample-purification for GC-MS analysis of ractopamine in swine tissue. J Anim Sci, 2006, 84: 1248–1251
Ding S Y, Shen J Z, Zhang S X, et al. Determination of chloramphenicol residue in fish and shrimp tissues by gas chromatography with a micrcell electron capture detector. J AOAC Int, 2005, 88: 57–60
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Zhang, Z., Liu, J., Yao, Y. et al. A competitive dual-label time-resolved fluoroimmunoassay for the simultaneous determination of chloramphenicol and ractopamine in swine tissue. Chin. Sci. Bull. 56, 1543–1547 (2011). https://doi.org/10.1007/s11434-011-4412-4
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DOI: https://doi.org/10.1007/s11434-011-4412-4