Abstract
This study was conducted to investigate the effects of Chinese herbs capable of replenishing qi, nourishing yin and activating blood circulation and their compatibility on differentially expressed genes of ischemic myocardium which were selected from differential expression profile we had established before, and to explore the underlying mechanism. The acute myocardial infarction (AMI) model was established by ligating the left anterior descending (LAD) coronary artery, then the model rats were randomly divided into the model group, the Metoprolol group, the replenishing qi nourishing yin (RN) group, the activating blood circulation (AB) group, and the replenishing qi, nourishing yin and activating blood circulation (RA) group. In addition, the normal group and the sham group were set up. The rats of medication groups were administered by intragastric gavage with corresponding drugs on the second day after operations, and the rats of the normal group and the sham group were given normal saline as the same time. Then the ischemic hearts were harvested on the 8th day after operation. The myocardial pathomorphological changes were observed under a light microscope. The mRNA changes of target genes such as COX5a and ATP5e were detected using Real-time fluorescence quantitative PCR (Q-PCR), and the activities of related enzymes were detected by colorimetric assay. The main results were as follows: the histological changes were observed by HE staining, and cardiocyte swelling, inflammatory cell infiltration and cytolysis were showed in regional ischemic myocardium of the model group, while the pathomorphological changes in all medication groups did not show obvious changes. Two genes related to energy metabolism, COX5a and ATP5e, were selected as the target genes which were down-regulated at the mRNA level in the medication groups. The activities of correlative functional enzymes also decreased in the RA group compared to that in the model group accordingly (P<0.05). The results indicated that the abnormal expression of genes involved in energy metabolism pathways could be one of the molecular mechanisms of AMI. The compatibility of Chinese herbs capable of replenishing qi, nourishing yin and activating blood circulation affects the expression of energy-relative gene COX5a, ATP5e, which is probably the mechanism of myocardial preservation, and is more effective than single herb of replenishing qi and nourishing yin or activating blood circulation.
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References
Wolff A A, Rotmensch H H, Stanley W C, et al. Metabolic approaches to the treatment of ischemic heart disease: The clinicians’ perspective, Heart Fail Rev, 2002, 7: 187–203
Yang Y M, Yang N. Discussion of important link from progresses in coronary heart disease therapy with TCM (in Chinese). Chinese J Integr Tradit West Med, 2002, 22: 863–865
Shao N Q, Zhu X X. Pharmacological progresses in hypoxia-ischemia of myocardium (in Chinese). J Pract Tradit Chinese Intern Med, 2007, 21: 3–4
Guo C Y, Yin H J, Jiang Y R, et al. Differential gene expression profile in ischemic myocardium of Wistar rats with acute myocardial infarction. Chinese Sci Bull, 2008, 53: 2488–2495
Zhang R S, Wang J S. Discussion of some problems about rat model with myocardial infarction (in Chinese). J Shanxi Med Univ, 2004, 35: 13–15
Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)). Methods, 2001, 25: 402–408
Arocho A, Chen B, Ladanyi M Validation of the 2-DeltaDeltaCt calculation as an alternate method of data analysis for quantitative PCR of BCR-ABL P210 transcripts. Diagn Mol Pathol, 2006, 15: 56–61
Zhang J, Liem D A, Mueller M, et al. Altered proteome biology of cardiac mitochondria under stress conditions. J Proteome Res, 2008, 7: 2204–2214
Sammut I A, Burton K, Balogun E, et al. Time-dependent impairment of mitochondrial function after storage and transplantation of rabbit kidneys. Transplantation, 2000, 69: 1265–1275
Zhang M, Zhou S H. Therapy progresses in energy metabolism impairment of myocardial ischemia (in Chinese). Chinese Heart J, 2006, 18: 467–469
Lee S D, Kuo W W, Lin J A, et al. Effects of long-term intermittent hypoxia on mitochondrial and Fas death receptor dependent apoptotic pathways in rat hearts. Int J Cardiol, 2007, 116: 348–356
Cui X S, Li X Y, Jeong Y J, et al. Gene expression of Cox5a, 5b,or 6b1 and their roles in preimplantation mouse embryos. Biol Reprod, 2006, 74: 601–610
Weinberg J M, Venkatachalam M A, Roeser N F, et al. Mitochondrial dysfunction during hypoxia/reoxygenation and its correction by anaerobic metabolism of citric acid cycle intermediates. Proc Natl Acad Sci USA, 2000, 97: 2826–2831
Liang W S, Tang L X, Yang Z C, et al. Changes of myocardial mitochondrial F0F1-ATPase activity and its effects on energy metabolism in early stage of severe burns (in Chinese). Acta Academiae Medicine Militaris Tertiae, 2001, 23: 780–782
Yin H J, Zhang Y, Yang L H, et al. The effects of PQS on glucose transport, GLUT4 translocation and CAP mRNA expression of adipocytes (in Chinese). Chinese Pharmacol Bull, 2007, 23: 1332–1337
Yin H J, Zhang Y, Jiang Y R, et al. Effect of Folium panax quinque-folium saponins on apoptosis of cardiac muscle cells and apoptosis-related gene expression in rats with acute myocardial infarction (in Chinese). Chinese J Integr Tradit West Med, 2005, 25: 232–235
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Supported by the National Natural Science Foundation of China (Grant No. 30572430)
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Yin, H., Guo, C. & Shi, D. Effects of Chinese herbs capable of replenishing qi, nourishing yin and activating blood circulation and their compatibility on differentially expressed genes of ischemic myocardium. Chin. Sci. Bull. 54, 3278–3282 (2009). https://doi.org/10.1007/s11434-009-0484-9
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DOI: https://doi.org/10.1007/s11434-009-0484-9