Skip to main content
SpringerLink
Log in

Microcalorimetric investigation of effect of berberine alkaloids from Coptis chinensis Franch on intestinal diagnostic flora growth

  • Articles/Physical Chemistry
  • Published:
Chinese Science Bulletin

Abstract

The inhibitory effect of three berberine alkaloids (BAs) from Coptis chinensis Franch, a traditional Chinese medicinal (TCM) herb, on Bifidobacterium adolescentis growth was investigated by microcalorimetry. The power-time curves of B. adolescentis with and without BAs were acquired, meanwhile the extent and duration of inhibitory effect on the metabolism were evaluated by the growth rate constant (k), half inhibitory ratio (IC50), maximum heat-output power (P max), peak time of maximum heat-output power (t p) and total heat production (Q t). k, P max and Q t decreased, and t p was prolonged with the increase of BAs concentration. The IC50 of BAs is 806 μg/mL for berberine, 341 μg/mL for coptisine and 236 μg/mL for palmatine. The sequence of antimicrobial activity of BAs is berberine < coptisine < palmatine. Combined with previous studies, it could be shown that the sequences of antimicrobial activity of BAs on both Bacillus shigae and Escherichia coli are berberine > coptisine > palmatine. The structure-function relationship of BAs indicates that the functional group methylenedioxy or methoxyl at C2 and C3 might be the major group inducing the activities of BAs on E. coli and B. adolescentis. Meanwhile, the substituent groups at C2, C3, C9 and C10 almost have equal effect on B. shigae.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. China Pharmacopoeia Committee, Pharmacopoeia of the People’s Republic of China (in Chinese). 1st Div. Beijing: China Chemical Industry Press, 2005. 213–214

    Google Scholar 

  2. Ding Z M. Pharmacodynamic Action & Clinic of Chinese Medicinal Materials (in Chinese). Beijing: China Medicine Technology Press, 1999. 54–56

    Google Scholar 

  3. Guo X H. Foundation & Application of Probiotics (in Chinese). Beijing: Beijing Science & Technology Press, 2002. 7–10

    Google Scholar 

  4. Ingemar W. Isothermal microcalorimetry in applied biology. Therm Acta, 2002, 394(1): 305–311

    Article  Google Scholar 

  5. Shen X S, Liu Y, Zhou C P, et al. Thermochemical studies on the quality antibacterial effect relationship of fluorquinolones. Acta Chim Sin (in Chinese), 2000, 58(11): 1463–1466

    Google Scholar 

  6. Ingemar W. Isothermal microcalorimetry for the characterization of interactions between drugs and biological materials. Therm Acta, 1995, 267: 45–59

    Article  Google Scholar 

  7. Graham B. Applications of isothermal microcalorimetry in the pharmaceutical sciences. Therm Acta, 1995, 248:117–129

    Article  Google Scholar 

  8. Wu Y W, Gao W Y, Xiao X H, et al. Calorimetric investigation of the effect of hydroxyanthraquinones in Rheum officinale Baill on Staphylococcus aureus growth. Therm Acta, 2005, 429(2): 167–170

    Article  Google Scholar 

  9. Xie C L, Tang H K, Song Z H, et al. Microcalorimetric study of bacterial growth. Therm Acta, 1988, 123: 33–41

    Article  Google Scholar 

  10. Yan D, Xiao X H, Jin C, et al. Microcalorimetric investigation of the effect of berberine alkaloids from Coptis chinensis Franch on Staphylococcus aureus growth. Sci China Ser B-Chem, 2008, 51(7): 640–645

    Article  Google Scholar 

  11. Katarzyna L, Bartlomiej P, Jerzy D. Microcalorimetry as a possible tool for phenanthrene toxicity evaluation to eukaryotic cells. Therm Acta, 2004, 411(2): 181–186

    Article  Google Scholar 

  12. Liu Y, Yan C N, Wang T Z, et al. Kinetics of the toxic action of Pb2+ on Rhizopus nigricans as studied by microcalorimetry. Therm Acta, 1999, 333(2): 103–108

    Article  Google Scholar 

  13. Tan A M, Huang Y Q, Qu S S. Determination of the respiratory burst of polymorphonuclear leukocytes by microcalorimetry. J Biochem Biophys Methods, 1998, 37(1): 91–94

    Article  Google Scholar 

  14. Tan A M, Xie C L, Qu S S. Microcalorimetric study of mitochondria isolated from fish liver tissue. J Biochem Biophys Methods, 1996, 31(3): 189–193

    Article  Google Scholar 

  15. Liu Y, Tzitschung T, Tahn C Z, et al. Microcalorimetric studies on the metabolism of Chlorella vulgaris. Chemosphere, 2000, 40(8): 845–849

    Article  Google Scholar 

  16. Liu G S, Liu Y, Chen X D, et al. Study on interaction between T4 phage and Escherichia coli B by microcalorimetric method. J Virol Methods, 2003, 112(1): 137–143

    Article  Google Scholar 

  17. Hu C Q, Liu Y N, Wang S M, et al. A microcalorimetric study on the effect of inhibitors on enzyme: Determination of inhibition constant k i. Chin Sci Bull, 1984, 29(5): 610–610

    Google Scholar 

  18. Yan D, Jin C, Xiao X H, et al. Investigation of the effect of berberins alkalokds in Coptis chinensis Franch on Bacillus shigae growth by microcalorimetry. Sci China Ser B-Chem, 2007, 50(5): 638–642

    Article  Google Scholar 

  19. Yan D, Jin C, Xiao X H, et al. Antimicrobial properties of berberines alkaloids in Coptis chinensis Franch by microcalorimetry. J Biochem Biophys Methods, 2008, 70(6): 845–849

    Article  Google Scholar 

  20. Li X, Liu Y, Wu J, et al. Microcalorimetric study of Staphylococcus aureus growth affected by selenium compounds. Therm Acta, 2002, 387(1): 57–61

    Article  Google Scholar 

  21. Su X Y. Biological Fingerprinting Analysis for Probing the Interaction of DNA and Extracts of Traditional Chinese Medicines (in Chinese). Dissertation for the Doctoral Degree. Dalian: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 2006. 58–64

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Chinese Medicine, 302 Hospital of People’s Liberation Army, Beijing, 100039, China

    Dan Yan, Li Wei, XiaoHe Xiao & DanLei Zhou

  2. Chongqing Institute of Technology, Chongqing, 400050, China

    YuMei Han

Authors
  1. Dan Yan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Li Wei
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. XiaoHe Xiao
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. DanLei Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. YuMei Han
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to XiaoHe Xiao.

Additional information

Supported by the National Natural Science Foundation of China (Grant Nos. 30625042 and 30873385) and Key Projects of National Science & Technology Pillar Program of China in the 11th Five-Year Period (Grant No. 2007BAI40B05)

About this article

Cite this article

Yan, D., Wei, L., Xiao, X. et al. Microcalorimetric investigation of effect of berberine alkaloids from Coptis chinensis Franch on intestinal diagnostic flora growth. Chin. Sci. Bull. 54, 369–373 (2009). https://doi.org/10.1007/s11434-009-0001-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11434-009-0001-1

Keywords

Search

Navigation