Skip to main content
SpringerLink
Log in

Effect of Dy3+on osteogenic and adipogenic differentiation of mouse primary bone marrow stromal cells and adipocytic trans-differentiation of mouse primary osteoblasts

  • Articles/Inorganic Chemistry
  • Published:
Chinese Science Bulletin

Abstract

A series of experimental methods including 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) test, alkaline phosphatase (ALP) activity measurement, mineralized function, Oil Red O stain and measurement were employed to assess the effect of Dy3+ on the osteogenic and adipogenic differentiation of mouse primary bone marrow stromal cells (BMSCs) and the adipogenic trans-differentiation of mouse primary osteoblasts (OBs). The results showed that Dy3+ had no effect on BMSC proliferation at concentrations of 1×10−8 and 1×10−5 mol/L, but inhibited BMSC proliferation at other concentrations. Dy3+ had no effect on OB proliferation at concentrations of 1×10−10 and 1×10−9 mol/L, but inhibited OB proliferation at other concentrations. Dy3+ had no effect on the osteogenic differentiation of BMSCs at concentrations of 1×10−9 and 1×10−7 mol/L, and promoted osteogenic differentiation of BMSCs at other concentrations at the 7th day. The osteogenic differentiation of BMSCs was inhibited by Dy3+ at concentration of 1×10−5 mol/L at the 14th day, but promoted osteogenic differentiation of BMSCs at concentrations of 1×10−9, 1×10−8, 1×10−7 and 1×10−6 mol/L with the maximal effect at concentration of 10−6 mol/L. Dy3+ promoted mineralized function of BMSCs at any concentration. Dy3+ had no effect on adipogenic differentiation of BMSCs at concentration of 1×10−7 mol/L, but inhibited adipogenic differentiation of BMSCs at other concentrations. Dy3+ inhibited adipocytic trans-differentiation of OBs at any concentration, suggesting that Dy3+ had protective effect on bone and the protective effect on bone may be mediated by modulating differentiation of BMSCs away from the adipocyte and inhibiting adipocytic trans-differentiation of OBs which may promote differentiation and mineralization of OBs. These results may be valuable for better understanding the mechanism of the effect of Dy3+ on pathogenesis of osteoporosis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Wattel A, Kamel S, Mentaverri R, et al. Potent inhibitory effect of naturally occurring flavonoids quercetin and kaemperol on in vitro osteoclastic bone resorption. Biochem Pharmacol, 2003, 65(1): 35–42

    Article  PubMed  CAS  Google Scholar 

  2. Saltman P D, Strause L G. The role of trace minerals in osteoporosis. J Am Coll Nutr, 1993, 12(4): 384–389

    PubMed  CAS  Google Scholar 

  3. Moonga B S, Dempster D W. Zinc is a potent inhibitor of osteoclastic bone resorption in vitro. J Bone Miner Res, 1995, 10(3): 453–457

    Article  PubMed  CAS  Google Scholar 

  4. Malgaroli A, Meldolesi J, Zambonin-Zallone A, et al. Control of cytosolic free calcium in chicken and rat osteoclasts. J Biol Chem, 1989, 264(24): 14342–14347

    PubMed  CAS  Google Scholar 

  5. Wang K. The analogy in chemical and biological behavior between non-essential ions compared with essential ions. South Afr J Chem, 1997, 54: 232–239

    Google Scholar 

  6. Jha A M, Singh A C. Clastogenicity of lanthanides-induction of micronuclei in root tips of vicia faba. Mutation Res, 1994, 322(3): 169–172

    Article  PubMed  CAS  Google Scholar 

  7. Quarles L D, Hartle J E, Middleton J P. Aluminum-induced DNA synthesis in osteoblasts: mediation by a G-protein coupled cation sensing mechanism. J Cell Biochem, 1994, 56(1): 106–117

    Article  PubMed  CAS  Google Scholar 

  8. Li R C, Yang H W, Wang K. The long-term effects of oral lanthanum supplementation at a low dose to rats on lanthanum accumulation in the bone and bone microstructure. J Peking Uni (Heal Sci), 2003, 35(6): 622–624

    CAS  Google Scholar 

  9. Zhang J C, Xu S J, Wang K, et al. Effects of the rare earth ions on bone resorbing function of rabbit mature osteoclasts in vitro. Chin Sci Bull, 2003, 48(20): 2170–2175

    Article  CAS  Google Scholar 

  10. Zhang J C, Li X X, Xu S J, et al. The effects of rare earth ions on proliferation, differentiation and function expression of osteoblasts in vitro. Prog Nat Sci, 2004, 14(4): 404–409

    Article  Google Scholar 

  11. Zhang D W, Zhang J C, Chen Y, et al. Effects of lanthanum and gadolinium on proliferation and differentiation of primary osteoblasts. Prog Nat Sci, 2007, 17(5): 618–623

    Article  CAS  Google Scholar 

  12. Ahdjoudj S, Fromigue O, Marie P J. Plasticity and regulation of human bone marrow stromal osteoprogenitor cells: potential implication in the treatment of age-related bone loss. Histol Histopathol, 2004, 19(1): 151–157

    PubMed  CAS  Google Scholar 

  13. Dominici M, Hofmann T J, Horwitz E M. Bone marrow mesenchymal cells: biological properties and clinical applications. J Biol Regul Homeost Agents, 2001, 15(1): 28–37

    PubMed  CAS  Google Scholar 

  14. Li X, Cui Q, Kao C, et al. Lovastatin inhibits adipogenic and stimulates osteogenic differentiation by suppressing PPARγ2 and increasing Cbfa1/Runx2 expression in bone marrow mesenchymal cell cultures. Bone, 2003, 33(4): 652–659

    Article  PubMed  CAS  Google Scholar 

  15. Beresford J N, Bennett J H, Devlin C, et al. Evidence for an inverse relationship between the differentiation of adipocytic and osteogenic cells in rat marrow stromal cell cultures. J Cell Sci, 1992, 102(2): 341–351

    PubMed  CAS  Google Scholar 

  16. Nuttall M E, Gimble J M. Is there a therapeutic opportunity to either prevent or treat osteopenic disorders by inhibiting marrow adipogenesis? Bone, 2000, 27(2): 177–184

    Article  PubMed  CAS  Google Scholar 

  17. Song C L, Dang G T. Adipocytes in marrow space and osteoporosis. Chin J Osteoporos, 2002, 8(3): 266–269

    Google Scholar 

  18. Verma S, Rajaratnam J H, Denton J, et al. Adipoctyic proportion of bone marrow is inversely related to bone formation in osteoporosis. J Clin Pathol, 2002, 55(9): 693–698

    Article  PubMed  CAS  Google Scholar 

  19. Thomas O C, Kathleen C M, Bruce E, et al. Osteocalcin production in primary osteoblast cultures derived from normal and Hyp mice. Endocrinol, 1998, 139(1): 36–43

    Google Scholar 

  20. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods, 1983, 65(1–2): 55–63

    Article  PubMed  CAS  Google Scholar 

  21. Li X H, Zhang J C, Sui S F, et al. Effects of daidzin, genistin and glycitin on the osteogenic and adipogenic differentiation of bone marrow stromal cells and the adipocytic trans-differentiation of osteoblasts. Acta Pharma Sinica, 2005, 26(9): 1081–1086

    Article  CAS  Google Scholar 

  22. Gori F, Divieti P, Demay M. Cloning and characterization of a novel WD-40 repeat protein that dramatically accelerates osteoblastic differentiation. J Biol Chem, 2001, 276(49): 46515–46522

    Article  PubMed  CAS  Google Scholar 

  23. Katherine A K, Jeffrey M G. 1,25-Dihydroxy vitamin D3 inhibits adipocyte differentiation and gene expression in murine bone marrow stromal cell clones and primary cultures. Endocrinol, 1998, 139(5): 2622–2628

    Article  Google Scholar 

  24. Ailhaud G, Grimaldi P, Negrel R. Cellular and molecular aspects of adipose tissue development. Annu Rev Nutr, 1992, 12: 207–233

    Article  PubMed  CAS  Google Scholar 

  25. Benayahu D, Zipori D, Wientroub S. Marrow adipocytes regulate growth and differentiation of osteoblasts. Biochem Biophys Res Comm, 1993, 197(3): 1245–1252

    Article  PubMed  CAS  Google Scholar 

  26. Kelly K A, Tanaka S, Baron R, et al. Murine bone marrow stromally derived BMS2 adipocytes support differentiation and function of osteoclast-like cells in vitro. Endocrinol, 1998, 139(4): 2092–2101

    Article  CAS  Google Scholar 

  27. Sakaguchi K, Morita I, Murota S. Relationship between the ability to support differentiation of osteoclast-like cells and adipogenesis in murine stromal cells derived from bone marrow. Prostaglandins Leukotrienes and Essential Fatty Acids, 2000, 62(5): 319–327

    Article  CAS  Google Scholar 

  28. Benayahu D, Peled A, Zipori D. Myeloblastic cell line expresses osteoclastic properties following coculture with marrow stromal adipocytes. J Cell Biochem, 1994, 56: 374–384

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Chemical Biology Laboratory, College of Chemistry and Environmental Science, Hebei University, Baoding, 071002, China

    JinChao Zhang, DanDan Liu & ShiGang Shen

  2. Department of Natural Products Chemistry, Shenyang Pharmaceutical University, Shenyang, 110016, China

    DaWei Zhang

  3. Department of Biology and Chemistry, City University of Hong Kong, Hong Kong, China

    MengSu Yang

  4. B-Ultrasound Room, Affiliated Hospital of Hebei University, Baoding, 071000, China

    Jing Sun

Authors
  1. JinChao Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. DanDan Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jing Sun
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. DaWei Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. ShiGang Shen
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. MengSu Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to JinChao Zhang.

Additional information

Supported by the Foundation for Key Program of Ministry of Education of China (Grant No. 208018)

About this article

Cite this article

Zhang, J., Liu, D., Sun, J. et al. Effect of Dy3+on osteogenic and adipogenic differentiation of mouse primary bone marrow stromal cells and adipocytic trans-differentiation of mouse primary osteoblasts. Chin. Sci. Bull. 54, 66–71 (2009). https://doi.org/10.1007/s11434-008-0503-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11434-008-0503-2

Keywords

Search

Navigation