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Genetic diversity and construction of core collection in Chinese wheat genetic resources

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  • Crop Germplasm Resources
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Chinese Science Bulletin

Abstract

Genetic diversity among 5029 accessions representing a proposed Chinese wheat core collection was analyzed using 78 pairs of fluorescent microsatellite (SSR) primers mapped to 21 chromosomes. A stepwise hierarchical sampling strategy with priority based on 4×105 SSR data-points was used to construct a core collection from the 23090 initial collections. The core collection consisted of 1160 accessions, including 762 landraces, 348 modern varieties and 50 introduced varieties. The core accounts for 23.1% of the 5029 candidate core accessions and 5% of the 23090 initial collections, but retains 94.9% of alleles from the candidate collections and captures 91.5% of the genetic variation in the initial collections. These data indicate that it is possible to maintain genetic diversity in a core collection while retaining fewer accessions than the accepted standard, i.e., 10% of the initial collections captured more than 70% of their genetic diversity. Estimated genetic representation of the core constructed by preferred sampling (91.5%) is much higher than that by random sampling (79.8%). Both mean genetic richness and genetic diversity indices of the landraces were higher than those of the modern varieties in the core. Structure and principal coordinate analysis revealed that the landraces and the modern varieties were two relatively independent subpopulations. Strong genetic differentiation associated with ecological environments has occurred in the landraces, but was relatively weak in the modern cultivars. In addition, a mini-core collection was constructed, which consisted of 231 accessions with an estimated 70% representation of the genetic variation from the initial collections. The mini-core has been distributed to various research and breeding institutes for detailed phenotyping and breeding of genetic introgression lines.

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References

  1. Dong Y S, Cao Y S, Zhang X Y, et al. Establishment of candidate core collections in Chinese common wheat germplasm. J Plant Genet Resour (in Chinese with English abstract), 2003, 4: 1–8

    Google Scholar 

  2. Tanksley S D, McCouch S R. Seed bank and molecular maps: Unlocking genetic potential from the wild. Science, 1997, 277: 1063–1066

    Article  PubMed  CAS  Google Scholar 

  3. Frankel O H. Genetic perspectives of germplasm conservation. In: Arber W, Limensee K, Peacock W J, et al, eds. Genetic Manipulation: Impact on Man and Society. Cambridge: Cambridge University Press, 1984. 161–170

    Google Scholar 

  4. Frankel O H, Brown A H D. Plant genetic resources today: A critical appraisal. In: Holden J H W, Williams J T, eds. Crop Genetic Resources: Conservation & Evaluation. London: George Allen & Urwin Ltd, 1984. 249–257

    Google Scholar 

  5. Brown A H D. Core collections: A practical approach to genetic resources management. Genome, 1989, 31: 818–824

    Google Scholar 

  6. Brown A H D. The case for core collections. In: Brown A H D, Frankel O H, Marshall D R, et al, eds. The Use of Plant Genetic Resources. Cambridge: Cambridge University Press, 1989. 136–156

    Google Scholar 

  7. Hamon S, van Stolen D H. Characterization and evaluation of okra. In: Brown A H D, Frankel O H, Marshall D R, et al, eds. The Use of Plant Genetic Resources. Cambridge: Cambridge University Press, 1989. 173–196

    Google Scholar 

  8. Corley H C, William F A. Evaluation of a core collection to identify resistance to late leaf spot in peanut. Crop Sci, 1995, 35: 1700–1702

    Google Scholar 

  9. Upadhyaya H D, Bramel P J, Singh S. Development of a chickpea core subset using geographic distribution and quantitative traits. Crop Sci, 2001, 41: 206–210

    Google Scholar 

  10. Hao C Y, Zhang X Y, Wang L F, et al. Genetic diversity and core collection evaluations in common wheat germplasm from the Northwestern Spring Wheat Region in China. Mol Breed, 2006, 17: 69–77

    Article  CAS  Google Scholar 

  11. Hu J, Zhu J, Xu H M. Methods of constructing core collections by stepwise cluster with three sampling strategies based on genotypic values of crops. Theor Appl Genet, 2000, 101(1–2): 264–268

    Article  CAS  Google Scholar 

  12. Wang J C, Hu J, Liu N N, et al. Investigation of combining plant genotypic values and molecular marker information for constructing core subsets. J Integr Plant Biol, 2006, 48: 1371–1378

    Article  CAS  Google Scholar 

  13. Yonezawa K, Nomura T, Morishima H. Sampling strategies for use in stratified germplasm collections. In: Hodekin T, Brown A H D, van Hintum T J L, et al, eds. Core Collections of Plant Genetic Resources. Chichester: Wiley-Sayce Publication, 1995. 35–54

    Google Scholar 

  14. Brown A H D, Grace J P, Speer S S. Designation of a “core” collection of perennial Glycine. Soybean Genetics Newsletter, 1987, 14: 59–70

    Google Scholar 

  15. van Hintum T J L. Comparison of marker systems and construction of a core collection in a pedigree of European spring barley. Theor Appl Genet, 1994, 89: 991–997

    Article  Google Scholar 

  16. Li Z C, Zhang H L, Zeng Y W, et al. Study on sampling schemes of core collection of local varieties of rice in Yunnan, China. Sci Agric Sin (in Chinese), 2000, 33(5): 1–7

    Google Scholar 

  17. Ellegren H. Microsatellites: Simple sequences with complex evolution. Nat Rev Genet, 2004, 5: 435–445

    Article  PubMed  CAS  Google Scholar 

  18. Balfourier F, Roussel V, Strelchenko P, et al. A worldwide bread wheat core collection arrayed in a 384-well plate. Theor Appl Genet, 2007, 114: 1265–1275

    Article  PubMed  Google Scholar 

  19. Zhang X Y, Pang B S, You G X, et al. Allelic variation and genetic diversity at Glu-1 loci in Chinese wheat (Triticum aestivum L.) germplasms. Sci Agric Sin (in Chinese), 2002, 35(11): 1302–1310

    CAS  Google Scholar 

  20. Zhang X Y, Tong Y P, You G X, et al. Hitchhiking effect mapping: A new approach for discovering agronomic important genes. Sci Agric Sin (in Chinese), 2006, 39(8): 1526–1535

    Google Scholar 

  21. Zhuang Q S. Chinese Wheat Improvement and Pedigree Analysis (in Chinese). Beijing: Agricultural Press, 2003

    Google Scholar 

  22. Institute of Crop Germplasm Resources, CAAS. Chinese Wheat Genetic Resource Catalogs. Beijing: China Agriculture Press, 1995.

    Google Scholar 

  23. Hao C Y, Wang L F, Dong Y C, et al. Comparison of fluorescence and silver-staining detection systems of microsatellite markers. Acta Agron Sin (in Chinese), 2005, 31: 144–149

    CAS  Google Scholar 

  24. Röder M S, Korzun V, Wendehake K, et al. A microsatellite map of wheat. Genetics, 1998, 149: 2007–2023

    PubMed  Google Scholar 

  25. Zhang X Y, Li C W, Wang L F, et al. An estimation of the minimum number of SSR alleles needed to reveal genetic relationships in wheat varieties. I. Information from large-scale planted varieties and corner-stone breeding parents in Chinese wheat improvement and production. Theor Appl Genet, 2002, 106: 112–117

    PubMed  CAS  Google Scholar 

  26. You G X, Zhang X Y, Wang L F. An estimation of the minimum number of SSR loci needed to reveal genetic relationships in wheat varieties: Information from 96 random samples with maximized genetic diversity. Mol Breed, 2004, 14: 397–406

    Article  Google Scholar 

  27. Nei M. Analysis of gene diversity in subdivided populations. Proc Natl Acad Sci USA, 1973, 70: 3321–3323

    Article  PubMed  CAS  Google Scholar 

  28. Hu J, Xu H M, Zhu J. A method of constructing core collection reserving special germplasm materials. J Biomathemat, 2001, 16(3): 348–352

    Google Scholar 

  29. Rohlf F J. NTSYS-pc: Numerical taxonomy and multivariate analysis system, Version 2.1. NY: Exeter Software, 2000

    Google Scholar 

  30. Pritchard J K, Stephens M, Donnelly P. Inference of population structure using multilocus genotype data. Genetics, 2000, 155: 945–959

    PubMed  CAS  Google Scholar 

  31. Spagonletti Z P L, Qualset C O. Evaluation of five strategies for obtaining a core subset from a large genetic resource collection of durum wheat. Theor Appl Genet, 1993, 87: 295–304

    Article  Google Scholar 

  32. Hao C Y, Wang L F, Zhang X Y, et al. Genetic diversity in Chinese modern wheat varieties revealed by microsatellite markers. Sci China Ser C-Life Sci, 2006, 49: 218–226

    Article  CAS  Google Scholar 

  33. Peleman, J D, van der Voort R. Breeding by design. Trends Plant Sci, 2003, 8: 330–334

    Article  PubMed  CAS  Google Scholar 

  34. Jia J Z. Applications of theories and approaches of plant genomics. Revi China Agricult Sci Technol (in Chinese), 1999, 2: 41–45

    Google Scholar 

  35. He G M, Luo X J, Tian F, et al. Haplotype variation in structure and expression of a gene cluster associated with a quantitative trait locus for improved yield in rice. Genome Res, 2006, 16: 618–626

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Key Laboratory of Crop Germplasm and Biotechnology, Ministry of Agriculture/The National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    ChenYang Hao, YuChen Dong, LanFen Wang, GuangXia You, HongNa Zhang, HongMei Ge, JiZeng Jia & XueYong Zhang

Authors
  1. ChenYang Hao
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  2. YuChen Dong
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  3. LanFen Wang
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  4. GuangXia You
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  5. HongNa Zhang
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  6. HongMei Ge
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  7. JiZeng Jia
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  8. XueYong Zhang
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Corresponding author

Correspondence to XueYong Zhang.

Additional information

Supported by the National Basic Research Program of China (Grant Nos. G19980202 and 2004CB117202)

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Hao, C., Dong, Y., Wang, L. et al. Genetic diversity and construction of core collection in Chinese wheat genetic resources. Chin. Sci. Bull. 53, 1518–1526 (2008). https://doi.org/10.1007/s11434-008-0212-x

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  • DOI: https://doi.org/10.1007/s11434-008-0212-x

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