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Inhibition of DNA restrictive endonucleases and Taq DNA polymerase by trimalonic acid C60

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  • Chemical Biology
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Chinese Science Bulletin

Abstract

Activities of trimalonic acid fullerene (TMA C60) on DNA restrictive enzymatic reaction were investigated by using two restrictive endonucleases Hind III and EcoR I and plasmid pEGFP-N1 with single restrictive site for both enzymes. Meanwhile, TMA C60 was also tested to clarify its effects on polymerase chain reaction (PCR) with the catalyst of Taq DNA polymerase and the template of plasmid pEGFP-N1. The products from restrictive reactions or PCR were detected by agarose gel electrophoresis. It was found that the product amounts from restrictive reactions or PCR decreased significantly with addition of TMA C60. The inhibition by TMA C60 was dose-dependent and IC50 values for reactions of Hind III, EcoR I and PCR were 16.3, 6.0 and 6.0 μmol/L, respectively. Addition of two scavengers of reactive oxygen species (ROS), L-ascorbic acid-2-phosphate ester magnesium and sodium azide at the concentrations of 2–10 mmol/L did not antagonize the activities of TMA C60 against PCR and two restrictive reactions. However, increase of Taq DNA polymerase amounts in PCR system antagonized the activities of TMA C60. These data implied that TMA C60 was able to inhibit the activities of the three above-mentioned enzymes involved in DNA metabolism, and that this inhibition probably did not correlate to ROS.

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Authors and Affiliations

  1. School of Life Science and Technology, Beijing Institute of Technology, Beijing, 100081, China

    Yang XinLin, Chen Zhe, Meng XianMei, Li Bo & Tan Xin

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  1. Yang XinLin
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  2. Chen Zhe
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  3. Meng XianMei
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  4. Li Bo
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  5. Tan Xin
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Correspondence to Yang XinLin.

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Supported by the Specialized Research Fund for the Doctoral Program of Higher Education (Grant No. 20030007011) and the Basic Research Foundation of Beijing Institute of Technology (Grant No. 000Y06)

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Yang, X., Chen, Z., Meng, X. et al. Inhibition of DNA restrictive endonucleases and Taq DNA polymerase by trimalonic acid C60 . CHINESE SCI BULL 52, 1802–1806 (2007). https://doi.org/10.1007/s11434-007-0241-x

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  • DOI: https://doi.org/10.1007/s11434-007-0241-x

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