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Study on peptide-peptide interaction using high-performance affinity chromatography and quartz crystal microbalance biosensor

  • Articles
  • Analytical Chemistry
  • Published:
Chinese Science Bulletin

Abstract

The specific interaction between sense and antisense peptides was studied by high-performance affinity chromatography (HPAC) and quartz crystal microbalance (QCM) biosensor. Fragment 1–14 of human interferon-β (hIFN-β) was chosen as sense peptide and its three antisense peptides (AS-IFN 1, AS-IFN 2, and AS-IFN 3) were designed according to the degeneracy of genetic codes. The affinity column was prepared with sense peptide as ligand and the affinity chromatographic behavior was evaluated. Glu-substituted antisense peptide (AS-IFN 3) showed the strongest binding to immobilized sense peptide at pH 7.5. A quartz crystal microbalance-flow injection analysis (QCM-FIA) system was introduced to investigate the recognition process in real-time. The equilibrium dissociation constants between sense peptide and AS-IFN 1, AS-IFN 2 and AS-IFN 3 measured 2.08×10−4, 1.31×10−4 and 2.22×10−5 mol/L, respectively. The mechanism study indicated that the specific recognition between sense peptide and AS-IFN 3 was due to sequence-dependent and multi-modal affinity interaction.

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Authors and Affiliations

  1. Beijing National Laboratory for Molecular Sciences, Laboratory of Analytical Chemistry for Life Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100080, China

    Luo Jia, Huang YanYan, Xiong ShaoXiang, Liu GuoQuan & Zhao Rui

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  1. Luo Jia
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  2. Huang YanYan
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  3. Xiong ShaoXiang
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  4. Liu GuoQuan
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  5. Zhao Rui
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Corresponding author

Correspondence to Zhao Rui.

Additional information

Supported by the National Natural Science Foundation of China (Grant Nos. 20575072, 20435030 and 90408018) and the Chinese Academy of Sciences (Grant No. KJCX2-SW-H06)

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Luo, J., Huang, Y., Xiong, S. et al. Study on peptide-peptide interaction using high-performance affinity chromatography and quartz crystal microbalance biosensor. CHINESE SCI BULL 52, 1311–1319 (2007). https://doi.org/10.1007/s11434-007-0175-3

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  • DOI: https://doi.org/10.1007/s11434-007-0175-3

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