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Multiple transgenes Populus xeuramericana ‘Guariento’ plants obtained by biolistic bombardment

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Chinese Science Bulletin

Abstract

A JERF36 regulation gene, a selection marker gene (NPT-II), and the foreign genes levansucrase (SacB), Vitreoscilla hemoglobin (vgb), and Binary coleopterus insect resistance (BtCry3A+OC-I) were co-transferred into Populus xeuramericana ‘Guariento’ using biolistic bombardment; 25 kanamycin resistant plants were obtained. The results of PCR and Southern hybridization showed that the foreign genes had been integrated into the genome of P. xeuramericana ‘Guariento’ and 5 genes were all transferred into 7 poplar plants. The results of a BtCry3A ELISA experiment indicated that the BtCry3A gene was expressed in the 7 transgenic popular plants, and these plants grew well on coastal saline land.

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Authors and Affiliations

  1. Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, China

    Wang JianGe, Su XiaoHua, Ji LiLi & Zhang BingYu

  2. College of Landscape and art, Jiangxi Agricultural University, Nanchang, 330045, China

    Wang JianGe

  3. Institute of Genetics and developmental biology, Chinese Academy of Sciences, Beijing, 100101, China

    Hu ZanMin

  4. Biotechnology Research Institute, Chinese Academy of Agricultural Science, Beijing, 100081, China

    Huang RongFeng

  5. Microbiology Institute, Chinese Academy of Sciences, Beijing, 100080, China

    Tian YingChuan

Authors
  1. Wang JianGe
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  2. Su XiaoHua
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  3. Ji LiLi
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  4. Zhang BingYu
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  5. Hu ZanMin
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  6. Huang RongFeng
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  7. Tian YingChuan
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Correspondence to Su XiaoHua.

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Wang, J., Su, X., Ji, L. et al. Multiple transgenes Populus xeuramericana ‘Guariento’ plants obtained by biolistic bombardment. CHINESE SCI BULL 52, 224–230 (2007). https://doi.org/10.1007/s11434-007-0034-2

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  • DOI: https://doi.org/10.1007/s11434-007-0034-2

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