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Identification of three novel noncoding RNAs from Drosophila melanogaster

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Chinese Science Bulletin

Abstract

Three novel small noncoding RNAs were identified from the conserved intronic regions of D. melanogaster by using comparative genomics method and molecular biology approach. One is a novel snoRNA in a combinated way, which displays structural features typical of C/D box snoRNA family and possesses a 10-nt-long rRNA antisense element for guiding the 2′-O-methylation of the D. melanogaster 28S rRNA at C2673. The other two are miRNAs whose predicted precursors adopt a stemloop structure characteristic of known miRNA. The two miRNAs genes appear to have ubiquitous expression profiles with ∼23-nt RNA transcripts detected by Northern blotting. Our study revealed 396 multi-species intronic conserved sequences (MCIS) that nested in the introns with a length from 100 to 500 bp. In addition to small RNA coding, the MCIS might function as cis-acting elements involved in gene transcription or post-transcriptional processing.

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Authors and Affiliations

  1. Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory for Biocontrol, Sun Yat-sen University, Guangzhou, 510275, China

    He Hualiang, Zhou Hui, Xiao Zhendong, Zeng Xianfen, Chen Junyu, Zheng Tao & Qu Lianghu

Authors
  1. He Hualiang
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  2. Zhou Hui
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  3. Xiao Zhendong
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  4. Zeng Xianfen
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  5. Chen Junyu
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  6. Zheng Tao
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  7. Qu Lianghu
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Corresponding author

Correspondence to Qu Lianghu.

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He, H., Zhou, H., Xiao, Z. et al. Identification of three novel noncoding RNAs from Drosophila melanogaster . CHINESE SCI BULL 51, 2737–2742 (2006). https://doi.org/10.1007/s11434-006-2202-1

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  • DOI: https://doi.org/10.1007/s11434-006-2202-1

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