Abstract
The genetic diversity of total 240 accessions of common wild rice coming from Gaozhou, Shaoguan, Zhanjiang, and other places in Guangdong, and Jiangxi, Fujian, Yunnan provinces, etc. in China and South-eastern Asia was evaluated by using 24 pairs of SSR primers. The results indicated that: (i) there were 17 polymorphic loci in 24 pairs of SSR primers, and the average percentage of polymorphic loci was 69%; the average total number of alleles, the average number of alleles per locus and the average number of alleles per polymorphic loci were 51.1, 2.04 and 2.43, respectively; (ii) the average gene diversity was 0.8447. There were remarkable differentiations among 5 populations of Gaozhou wild rice. All the above-mentioned genetic parameters indicated that Gaozhou wild rice of Pengshan Village (or Bei Toudong) of Zhenjiang Town should be a center of genetic differentiation and diversity of Gaozhou wild rice. Gaozhou wild rice is probably the largest center of genetic differentiation and diversity of common wild rice from Guangdong Province, South China and even the whole China.
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Ji Shuyi, Wang Jing, Wang Dianbei et al., A survey of the current status of Oryza rufipogon Griff., in Gaozhou City of Guangdong Province, in Studies and Applications of Wild Rice in China (Proceedings of the First National Conference on Wild Rice in China) (in Chinese), Beijing: Weather Press, 2004, 77–80.
Huang Kunde, Catalogue of Oryza rufipogon Griff., in Gaozhou City of Guangdong Province, in Studies and Applications of Wild Rice in China (the First National Conference on Wild Rice in China), Jiangxi, 2003, 108–110.
Wang Zhenshan, Zhu Lihuang, Liu Zhiyong et al., RFLP studies on natural populations of wild rice, Agricultural Biotechnological Journal (in Chinese), 1996, 4(2): 111–117.
Cai, H. W., Wang, X. K., Morishima, H., Genetic diversity of wild rice population, in Origin and Differentiation of Chinese Cultivated Rice (eds. Wang, X. K., Sun, C. Q.), Beijing: China Agricultural University Press, 1996, 154–156.
Sun, C. Q., Wang, X. K., Li, Z. C et al., Comparison of the genetic diversity of common wild rice (Oryza rufipogon Griff.) and cultivated rice (O. sativa L.) using RFLP markers, Theor. Appl. Genet., 2001, 102: 157–162.
Zhu Zuofeng, Sun Chuanqing, Fu Yongcai et al., Comparison of the genetic diversity of common wild rice and cultivated rice using SSR markers, Scientia Agriculture Sinica (in Chinese), 2002, 35(12): 1437–1441.
Yang Qingwen, Zhang Wanxia, Shi Jinxia et al., Genetic diversity and differentiation of Oryza rufipogon populations in Gaozhou, Journal of Plant Genetic Resources (in Chinese), 2004, 5(4): 315–319.
Wu, K. S., Tanksley, S. D., Abundance, polymorphism and genetic mapping of microsatellites in rice, Mol. Gen. Genet., 1993, (24): 223–235.
Fang Xuanjun, Wu Weiren, Tang Jiliang, Crop Breeding by DNA Marker-Assisted (in Chinese), Beijing: Science Press, 2001, 10–21.
McCouch, S. R., Temnykh, S., Lukashova, A. et al., Microsatellite markers in rice: Abundance, diversity, and applications, in Rice Genetics IV (eds. Khush, G. S., Brar, D. S., Hardy, B.), New Hampshire: Science Publishers, 2001, 117–135.
Zheng, K., Huang, N., Bennett, J. et al., PCR-based marker-assisted selection in rice breeding, in IRRI Discussion Paper Series No. 12, Manila: IRRI, 1995, 6–8.
Nei, M., Molecular Evolution Genetic, New York: Columbia University Press, 1987, 190–191.
Sokal, R. R., Michener, C. D., A statistic method for evaluating systematic relationships, Sci. Bull. Univ. Kanas., 1958, 28: 1409–1439.
Sun Chuanqing, Wang Xiangkun, Yoshimura A et al., A study of the genetic diversity of common wild rice (O. rufipogon Griff.) and cultivated rice (O. sativa L.) by RFLP analysis, Acta Genetica Sinica (in Chinese), 2000, 27(3): 227–234.
Sano, R., Morishima, H., Indica-japonica differentiation of rice cultivars viewed from variations in key characters and isozymes with special reference to landraces from the Himalayan hilly areas, Theor. Appl. Genet., 1992, 84: 266–274.
Gao Lizhi, Zhou Yi, Ge Song et al., Conservation countermeasure and current situation of genetic resources of common wild rice (Oryza rufipogon Griff.) from Guangxi Province, Scientia Agriculture Sinica (in Chinese), 1998, 31(1): 32–39.
Yang Qingwen, Studies on genetic diversity and conservation of common wild rice (Oryza rufipogon Griff.) in China (in Chinese), Dissertation for the Doctoral Degree, Chinese Agricultural University, 2004.
Cai Hongwei, Origination of cultivated rice in China and RFLP localization of Est-X locus (in Chinese), Dissertation for the Doctoral Degree, Beijing Agricultural University, 1993.
Wang Xiangkun, Sun Chuanqing, Li Zichao, Origination and evolution of cultivated rice in China, in Forum of Crop Breeding (ed. Liu Houli) (in Chinese), Beijing: Chinese Agricultural University Press, 2003, 1–24.
Huang Yanhong, Sun Xinli, Wang Xiangkun, Studies on scattered evolution of cultivated rice in Asia, Journal of Plant Genetic Resources (in Chinese), 2003, 4(2): 185–190.
Xiao, J. H., Grandillo, S., Ahn, S. N. et al., Genes from wild rice improve yield, Nature, 1996, 384: 223–224.
Li, D. J., Sun, C. Q., Fu, Y. C. et al., Identification and mapping of genes for improving yield from Chinese common wild rice (O. rufipogon Griff.) using advanced backcross QTL analysis, Chinese Science Bulletin, 2002, 47(18): 1533–1537.
Khush, G. S., Bacalangco, E., Ogawa, T., A new gene for resistance to bacterial blight from O. longistaminata, RGN, 1990, 7: 121–122.
Song, W. Y., Wang, G. L., Chen, L. L. et al., The rice disease resistance gene, Xa21, encodes a receptor kinase-like protein, Science, 1995, 270: 1804–1806.
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Li, C., Pan, D., Mao, X. et al. The genetic diversity of Gaozhou wild rice analyzed by SSR. CHINESE SCI BULL 51, 562–572 (2006). https://doi.org/10.1007/s11434-006-0562-1
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DOI: https://doi.org/10.1007/s11434-006-0562-1