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Pre-irradiation with low-dose 12C6+ beam significantly enhances the efficacy of AdCMV-p53 gene therapy in human non-small lung cancer

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Abstract

The combination of ionizing radiation and gene therapy has been investigated. However, there are very few reports about the combination of heavy-ion irradiation and gene therapy. To determine if the pre-exposure to low-dose heavy ion beam enhances the suppression of AdCMV-p53 on non-small lung cancer (NSLC), the cells pre-irradiated or non-irradiated were infected with 20, 40 MOI of AdCMV-p53. Survival fraction and the relative biology effect (RBE) were determined by clonogenic assay. The results showed that the proportions of p53 positive cells in 12C6+ beam induced AdCMV-p53 infected cells were more than 90%, which were significantly more than those in γ-ray induced AdCMV-p53 infected cells. The pre-exposure to low-dose 12C6+ beam significantly prevented the G0/G1 arrest and activated G2/M checkpoints. The pre-exposure to 12C6+ beam significantly improved cell to apoptosis. RBEs for the 12C6+ + AdCMV-p53 infection groups were 30%–60%, 20%–130% and 30%–70% more than those for the 12C6+-irradiated only, AdCMV-p53 infected only, and γ-irradiation induced AdCMVp53 infected groups, respectively. The data suggested that the pre-exposure to low-dose 12C6+ beam significantly promotes exogenous p53 expression in NSLC, and the suppression of AdCMV-p53 gene therapy on NSLC.

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Authors and Affiliations

  1. Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, 730000, China

    Liu Bing, Zhang Hong, Li WenJian, Li Qiang, Zhou GuangMing, Xie Yi, Hao JiFang, Min FengLing, Zhou QingMing & Duan Xin

  2. Graduate University of Chinese Academy of Sciences, Beijing, 100039, China

    Liu Bing, Xie Yi, Min FengLing, Zhou QingMing & Duan Xin

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  1. Liu Bing
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  2. Zhang Hong
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Correspondence to Liu Bing.

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Liu, B., Zhang, H., Li, W. et al. Pre-irradiation with low-dose 12C6+ beam significantly enhances the efficacy of AdCMV-p53 gene therapy in human non-small lung cancer. SCI CHINA SER G 50, 221–230 (2007). https://doi.org/10.1007/s11433-007-0012-3

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  • DOI: https://doi.org/10.1007/s11433-007-0012-3

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