References
Gallego-Bartolomé, J., Gardiner, J., Liu, W., Papikian, A., Ghoshal, B., Kuo, H.Y., Zhao, J.M.C., Segal, D.J., and Jacobsen, S.E. (2018). Targeted DNA demethylation of the Arabidopsis genome using the human TET1 catalytic domain. Proc Natl Acad Sci USA 115, E2125–E2134.
Law, J.A., and Jacobsen, S.E. (2010). Establishing, maintaining and modifying DNA methylation patterns in plants and animals. Nat Rev Genet 11, 204–220.
Liu, X.S., Wu, H., Ji, X., Stelzer, Y., Wu, X., Czauderna, S., Shu, J., Dadon, D., Young, R.A., and Jaenisch, R. (2016). Editing DNA methylation in the mammalian genome. Cell 167, 233–247.e17.
Luo, M., Platten, D., Chaudhury, A., Peacock, W.J., and Dennis, E.S. (2009). Expression, imprinting, and evolution of rice homologs of the polycomb group genes. Mol Plant 2, 711–723.
Morita, S., Noguchi, H., Horii, T., Nakabayashi, K., Kimura, M., Okamura, K., Sakai, A., Nakashima, H., Hata, K., Nakashima, K., et al. (2016). Targeted DNA demethylation in vivo using dCas9-peptide repeat and scFv-TET1 catalytic domain fusions. Nat Biotechnol 34, 1060–1065.
Papikian, A., Liu, W., Gallego-Bartolomé, J., and Jacobsen, S.E. (2019). Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems. Nat Commun 10, 729.
Wei, L., and Cao, X. (2016). The effect of transposable elements on phenotypic variation: insights from plants to humans. Sci China Life Sci 59, 24–37.
Zhang, L., Cheng, Z., Qin, R., Qiu, Y., Wang, J.L., Cui, X., Gu, L., Zhang, X., Guo, X., Wang, D., et al. (2012). Identification and characterization of an Epi-Allele of FIE1 reveals a regulatory linkage between two epigenetic marks in rice. Plant Cell 24, 4407–4421.
Acknowledgements
This work was supported by the National Key Research and Development Program of China (2016YFD0102003), the National Natural Science Foundation of China (31771872, 31788103 and 31970193), and Key Research Program of Frontier Sciences Chinese Academy of Sciences (QYZDY-SSW-SMC022). We thank Dr. Rongchen Wang for reading and revising this manuscript.
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Tang, S., Yang, C., Wang, D. et al. Targeted DNA demethylation produces heritable epialleles in rice. Sci. China Life Sci. 65, 753–756 (2022). https://doi.org/10.1007/s11427-021-1974-7
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DOI: https://doi.org/10.1007/s11427-021-1974-7