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Preliminary study of the urinary proteome in Li and Han ethnic individuals from Hainan

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Abstract

Biomarkers indicate changes associated with disease. Blood is relatively stable due to the homeostatic mechanisms of the body; however, urine accumulates metabolites from changes in the body, making it a better source for early biomarker discovery. The Li ethnic group is a unique minority ethnic group that has only lived on Hainan Island for approximately 5,000 years. Studies have shown that various specific genetic variations are different between the Li and Han ethnic groups. However, whether the urinary proteome between these two ethnic groups is significantly different remains unknown. In this study, differential urinary proteins were identified in the Li and Han ethnic groups using liquid chromatography tandem mass spectrometry (LC-MS/MS). In total, 1,555 urinary proteins were identified. Twenty-five of the urinary proteins were statistically significantly different, 16 of which have been previously reported to be biomarkers of many diseases, and that these significantly different proteins were caused by ethnic differences rather than random differences. Ethnic group differences may be an influencing factor in urine proteome studies and should be considered when human urine samples are used for biomarker discovery.

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Acknowledgements

This work was supported by the National Key Research and Development Program of China (2018YFC0910202, 2016YFC1306300), Beijing Natural Science Foundation (7172076), the Key Basic Research Program of the Ministry of Science and Technology of China (2013FY114100), Beijing Cooperative Construction Project (110651103), Beijing Normal University (11100704), and Peking Union Medical College Hospital (2016-2.27).

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  1. Department of Pathology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, China

    Fanshuang Zhang

  2. Department of Pathophysiology, Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, 100005, China

    Fanshuang Zhang, Xundou Li & Yanying Ni

  3. Department of Epidemiology and Statistics, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, Beijing, 100005, China

    Guangliang Shan

  4. Department of Biochemistry and Molecular Biology, Beijing Normal University, Gene Engineering Drug and Biotechnology Beijing Key Laboratory, Beijing, 100875, China

    Youhe Gao

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  1. Fanshuang Zhang
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Correspondence to Guangliang Shan or Youhe Gao.

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Compliance and ethics The author(s) declare that they have no conflict of interest. The study was approved by the local Ethics Committee at the Institute of Basic Medical Sciences at the Chinese Academy of Medical Sciences (NO. 029-2013).

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Zhang, F., Li, X., Ni, Y. et al. Preliminary study of the urinary proteome in Li and Han ethnic individuals from Hainan. Sci. China Life Sci. 63, 125–137 (2020). https://doi.org/10.1007/s11427-018-9485-4

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