Abstract
Proteins are the key players in many cellular processes. Their composition, trafficking, and interactions underlie the dynamic processes of life. Furthermore, diseases are frequently accompanied by malfunction of proteins at multiple levels. Understanding how biological processes are regulated at the protein level is critically important to understanding the molecular basis for diseases and often shed light on disease prevention, diagnosis, and treatment. With rapid advances in mass spectrometry (MS) instruments and experimental methodologies, MS-based proteomics has become a reliable and essential tool for elucidating biological processes at the protein level. Over the past decade, we have witnessed great expansion of knowledge of human diseases with the application of MS-based proteomic technologies, which has led to many exciting discoveries. Herein we review the recent progress in MS-based proteomics in biomedical research, including that in establishing disease-related proteomes and interactomes. We also discuss how this progress will benefit biomedical research and clinical diagnosis and treatment of disease.
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Acknowledgements
We thank Dr. Yi Wang, Dr. Sung Yun Jung and Dr. Jong Min Choi (Baylor College of Medicine) for their advice and critical readings, and Dr. Donald Norwood (MD Anderson Cancer Center) and Department of Scientific Publications at MD Anderson Cancer Center for editing the manuscript.
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Li, X., Wang, W. & Chen, J. Recent progress in mass spectrometry proteomics for biomedical research. Sci. China Life Sci. 60, 1093–1113 (2017). https://doi.org/10.1007/s11427-017-9175-2
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DOI: https://doi.org/10.1007/s11427-017-9175-2