Abstract
Dengue fever is a tropical disease and caused by dengue virus (DENV), which is transmitted by mosquitoes and infects about 400 million people annually. With the development of international trade and travel, China is facing a growing threat. Over 40 thousands of people were infected during the 2014 DENV outbreak in Guangdong. Neither licensed vaccine nor therapeutic drug has been available. In this report, we isolated two clinical DENV strains. The full-length genome was sequenced and characterized. We also applied a flavonoid, CPI, into an anti-DENV assay. Replication of viral RNA and expression of viral protein was all strongly inhibited. These results indicated that CPI may serve as potential protective agents in the treatment of patients with chronic DENV infection.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Bhatt, S., Gething, P.W., Brady, O.J., Messina, J.P., Farlow, A.W., Moyes, C.L., Drake, J.M., Brownstein, J.S., Hoen, A.G., Sankoh, O., Myers, M.F., George, D.B., Jaenisch, T., Wint, G.R., Simmons, C.P., Scott, T.W., Farrar, J.J., and Hay, S.I. (2013). The global distribution and burden of dengue. Nature 496, 504–507.
Chen, B., and Liu, Q. (2015). Dengue fever in China. Lancet 385, 1621–1622
Chen, X.L., Qu, L.B., Zhang, T., Liu, H.X., Yu, F., Yu, Y., Liao, X., and Zhao, Y.F. (2004). The nature of phosphorylated chrysin-protein interactions involved in noncovalent complex formation by electrospray ionization mass spectroscopy. Anal Chem 76, 211–217.
Chen, X.L., Yu, Z.Q., Qu, L.B., Yuan, J.W., Lu, J.S., and Zhao, Y.F. (2007). Phosphorylated modification of genistein and their interaction with lysozyme. Yao Xue Xue Bao 42, 396–400.
Coller, B.A., Clements, D.E., Bett, A.J., Sagar, S.L., and Ter Meulen, J.H. (2011). The development of recombinant subunit envelope-based vaccines to protect against dengue virus induced disease. Vaccine 29, 7267–7275.
Dejnirattisai, W., Wongwiwat, W., Supasa, S., Zhang, X., Dai, X., Rouvinski, A., Jumnainsong, A., Edwards, C., Quyen, N.T., Duangchinda, T., Grimes, J.M., Tsai, W.Y., Lai, C.Y., Wang, W.K., Malasit, P., Farrar, J., Simmons, C.P., Zhou, Z.H., Rey, F.A., Mongkolsapaya, J., and Screaton, G.R. (2015). A new class of highly potent, broadly neutralizing antibodies isolated from viremic patients infected with dengue virus. Nat Immunol 16, 170–177.
Fibriansah, G., Tan, J.L., Smith, S.A., de Alwis, R., Ng, T.S., Kostyuchenko, V.A., Jadi, R.S., Kukkaro, P., de Silva, A.M., Crowe, J.E., and Lok, S.M. (2015). A highly potent human antibody neutralizes dengue virus serotype 3 by binding across three surface proteins. Nat Commun 6, 6341.
Halstead, S.B. (2003). Neutralization and antibody-dependent enhancement of dengue viruses. Adv Virus Res 60, 421–467.
Higa, Y. (2011). Dengue vectors and their spatial distribution. Trop Med Health 39, 17–27.
Holmes, E.C., and Twiddy, S.S. (2003). The origin, emergence and evolutionary genetics of dengue virus. Infect Genet Evol 3, 19–28.
Imrie, A., Meeks, J., Gurary, A., Sukhbaatar, M., Truong, T.T., Cropp, C.B., and Effler, P. (2007). Antibody to dengue 1 detected more than 60 years after infection. Viral Immunol 20, 672–675.
Jiang, T., Yu, X.D., Hong, W.X., Zhou, W.Z., Yu, M., Deng, Y.Q., Zhu, S.Y., Qin, E.D., Wang, J., Qin, C.F., and Zhang, F.C. (2012). Co-circulation of two genotypes of dengue virus serotype 3 in Guangzhou, China, 2009. Virol J 9, 125.
Jin, X., Lee, M., and Shu, J. (2015). Dengue fever in China: an emerging problem demands attention. Emerg Microbes Infect 4, e3.
Johari, J., Kianmehr, A., Mustafa, M.R., Abubakar, S., and Zandi, K. (2012). Antiviral activity of baicalein and quercetin against the Japanese Encephalitis virus. Int J Mol Sci 13, 16785–16795.
Lyu, S.Y., Rhim, J.Y., and Park, W.B. (2005). Antiherpetic activities of flavonoids against herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) in vitro. Arch Pharm Res 28, 1293–1301.
Normile, D. (2013). Tropical medicine. Surprising new dengue virus throws a spanner in disease control efforts. Science 342, 415.
Pfaffl, M.W. (2001). A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29, e45.
Reed, L.J., and Muench, H. (1938). A simple method of estimating fifty per cent endpoints. Amjhyg 27, 493–497.
Rouvinski, A., Guardado-Calvo, P., Barba-Spaeth, G., Duquerroy, S., Vaney, M.C., Kikuti, C.M., Navarro Sanchez, M.E., Dejnirattisai, W., Wongwiwat, W., Haouz, A., Girard-Blanc, C., Petres, S., Shepard, W.E., Despres, P., Arenzana-Seisdedos, F., Dussart, P., Mongkolsapaya, J., Screaton, G.R., and Rey, F.A. (2015). Recognition determinants of broadly neutralizing human antibodies against dengue viruses. Nature 520, 109–113.
Saitou, N., and Nei, M. (1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
Sanderson, M.J., and Wojciechowski, M.F. (2000). Improved bootstrap confidence limits in large-scale phylogenies, with an example from Neo-Astragalus (Leguminosae). Syst Biol 49, 671–685.
Smith, S.A., de Alwis, A.R., Kose, N., Harris, E., Ibarra, K.D., Kahle, K.M., Pfaff, J.M., Xiang, X., Doranz, B.J., de Silva, A.M., Austin, S.K., Sukupolvi-Petty, S., Diamond, M.S., and Crowe, J.E. (2013). The potent and broadly neutralizing human dengue virus-specific monoclonal antibody 1C19 reveals a unique cross-reactive epitope on the bc loop of domain II of the envelope protein. MBio 4, e00873–00813.
Tamura, K., and Nei, M. (1993). Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol 10, 512–526.
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., and Kumar, S. (2011). MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28, 2731–2739.
Thomas, S.J., and Endy, T.P. (2011). Critical issues in dengue vaccine development. Curr Opin Infect Dis 24, 442–450.
Vasilakis, N., and Weaver, S.C. (2008). The history and evolution of human dengue emergence. Adv Virus Res 72, 1–76.
Wang, J., Su, H., Zhang, T., Du, J., Cui, S., Yang, F., and Jin, Q. (2014a). Inhibition of Enterovirus 71 replication by 7-hydroxyflavone and diisopropyl-flavon7-yl Phosphate. PLoS One 9, e92565.
Wang, J., Zhang, T., Du, J., Cui, S., Yang, F., and Jin, Q. (2014b). Anti-enterovirus 71 effects of chrysin and its phosphate ester. PLoS One 9, e89668.
Wu, J.Y., Lun, Z.R., James, A.A., and Chen, X.G. (2010). Dengue Fever in mainland China. Am J Trop Med Hyg 83, 664–671.
Wu, W., Bai, Z., Zhou, H., Tu, Z., Fang, M., Tang, B., Liu, J., Liu, L., and Chen, W. (2011). Molecular epidemiology of dengue viruses in southern China from 1978 to 2006. Virol J 8, 322.
Zandi, K., Lim, T.H., Rahim, N.A., Shu, M.H., Teoh, B.T., Sam, S.S., Danlami, M.B., Tan, K.K., and Abubakar, S. (2013). Extract of Scutellaria baicalensis inhibits dengue virus replication. BMC Complement Altern Med 13, 91.
Zandi, K., Teoh, B.T., Sam, S.S., Wong, P.F., Mustafa, M.R., and Abubakar, S. (2011). Antiviral activity of four types of bioflavonoid against dengue virus type-2. Virol J 8, 560.
Zhao, H., Deng, Y.Q., Hong, W.X., Yu, X.D., Jiang, T., Yu, M., Hu, F.Y., Zhu, S.Y., Li, X.F., Song, K.Y., Qin, E.D., Zhang, F.C., and Qin, C.F. (2012a). Complete genome sequence of dengue virus serotype 2 Cosmopolitan genotype strain in Guangdong, China. J Virol 86, 13808–13809.
Zhao, H., Yu, X.D., Zhang, X.Y., Jiang, T., Hong, W.X., Yu, M., Hu, F.Y., Zhu, S.Y., Qin, E.D., Deng, Y.Q., Qin, C.F., and Zhang, F.C. (2012b). Complete genome sequence of a dengue virus serotype 4 strain isolated in Guangdong, China. J Virol 86, 7021–7022.
Zhao, H., Zhao, L., Jiang, T., Li, X., Fan, H., Hong, W., Zhang, Y., Zhu, Q., Ye, Q., Tong, Y., Cao, W., Zhang, F., and Qin, C. (2014). Isolation and characterization of dengue virus serotype 2 from the large dengue outbreak in Guangdong, China in 2014. Sci China Life Sci 57, 1149–1155.
Author information
Authors and Affiliations
Corresponding authors
Additional information
This article is published with open access at link.springer.com
Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
Rights and permissions
Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0), which permits use, duplication, adaptation, distribution, and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
About this article
Cite this article
Du, J., Chen, Z., Zhang, T. et al. Inhibition of dengue virus replication by diisopropyl chrysin-7-yl phosphate. Sci. China Life Sci. 59, 832–838 (2016). https://doi.org/10.1007/s11427-016-5050-5
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11427-016-5050-5