Abstract
Ecological safety is a major consideration in the commercialization of transgenic fish. Development of sterile transgenic triploid fish through hybridization of transgenic tetraploid fish and transgenic diploid fish is a feasible way to solve this problem. The “all-fish” transgene, pbcAbcGHc, containing the black carp β-actin gene promoter and the open reading frame (ORF) of the black carp growth hormone (GH) gene was constructed and introduced into fertilized eggs of allotetraploid fish through microinjection. Contrast cultivation results showed that the growth rate of 150 day-old P0 black carp GH gene transgenic allotetraploid fish was much higher than that of controls. Sixty 150 day-old transgenic allotetraploid fish were assayed by PCR for transgene integration and 90% of fish were positive for the transgene. The transgene was detected in 13 of 20 sperm samples from male transgenic allotetraploid fish. RT-PCR detected transcription of the exogenous black carp GH gene in the muscle, liver, kidney and ovaries of the largest transgenic allotetraploid fish. This study has developed P0 black carp GH gene transgenic allotetraploid fish with a highly increased growth rate, which provides a solid foundation for the establishment of a pure line of transgenic allotetraploid fish and for the large scale production of sterile transgenic triploid fish.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Zhu Z, Li G, He L, et al. Novel gene transfer into fertilized eggs of goldfish (Carassius auratus). J Appl Ichthyol, 1985, 1: 31–34 10.1111/j.1439-0426.1985.tb00408.x, 1:CAS:528:DyaL2MXltFOnsL0%3D
Zeng Z Q, Zhu Z Y. Transgenes in F4 pMThGH-transgenic common carp (Cyprinus carpio L.) are highly polymorphic. Chin Sci Bull, 2001, 46: 143–148 10.1007/BF03187011, 1:CAS:528:DC%2BD3MXhvVOltrs%3D
Cachot J, Law M, Pottier D, et al. Characterization of toxic effects of sediment-associated organic pollutants using the λ transgenic Medaka. Environ Sci Technol, 2007, 41: 7830–7836 18075095, 10.1021/es071082v, 1:CAS:528:DC%2BD2sXhtFCisL%2FF
Fu C, Li D, Hu W, et al. Fast-growing transgenic common carp mounting compensatory growth. J Fish Biol, 2007, 71: 174–185 10.1111/j.1095-8649.2007.01401.x
Rosa C E, Fiqueiredo M A, Lanes C F, et al. Metabolic rate and reactive oxygen species production in different genotypes of GH-transgenic zebrafish. Comp Biochem Physiol B Biochem Mol Biol, 2008, 149: 209–214 17931920, 10.1016/j.cbpb.2007.09.010, 1:STN:280:DC%2BD2sjktF2qsA%3D%3D
Abad Z, Gonzalez R, Mendoza I, et al. Production of a high percentage of male offspring in growth-enhanced transgenic tilapia using Oreochromis aureus ZZ selected pseudofemales. Aquaculture, 2007, 270: 1–4 10.1016/j.aquaculture.2007.03.035
Zhu Z Y, Zeng Z Q. Open a door for transgenic fish to market. Biotech inf, 2000, 1: 1–6
Reynold M, Maria T H, Amilcar A, et al. Tilapia chromosomal growth hormone gene expression accelerates growth in transgenic zebrafish (Danio rerio). Elctron J Biotechnol, 2001, 4: 1–7
Sun X W, Liang L Q, Yan X C, et al. Research on transgenic fish as food. High Tech Lett, 1998, 3: 50–55
Wang J L. Transgenic organisms and biosafety. Chin J Ecol, 2006, 25: 314–317
Hu W, Wang Y P, Zhu Z Y. Progress in the evaluation of transgenic fish for possible ecological risk and its containment strategies. Sci China Ser C-Life Sci, 2007, 50: 573–579 10.1007/s11427-007-0089-y, 1:CAS:528:DC%2BD2sXhsVGgtbbN
Sundström L F, Lõhmus M, Johnsson J I, et al. Growth hormone transgenic coho salmon pay for growth potential with increased predation mortality. Proc R Soc Lond B, 2004, 271: 350–352 10.1098/rsbl.2004.0189
Li D L, Fu C Z, Hu W, et al. Rapid growth cost in “all-fish” growth hormone gene transgenic carp: reduced critical swimming speed. Chin Sci Bull, 2007, 52: 1501–1506 10.1007/s11434-007-0217-x, 1:CAS:528:DC%2BD2sXnsVCjsbg%3D
Sundström L F, Lõhmus M, Devlin R H. Migration and growth potential of coho salmon smolts: implications for ecological impacts from growth-enhanced fish. Ecol Appl, 2010, 20: 1372–1383 20666255, 10.1890/09-0631.1
Sundström L F, Lõhmus M, Tvmchuk W E, et al. Gene-environment interactions influence ecological consequences of transgenic animals. Proc Natl Acad Sci USA, 2007, 104: 3889–3894 17360448, 10.1073/pnas.0608767104
Jhingan E, Devlin R H, Iwama G K. Disease resistance, stress response and effects of triploidy in growth hormone transgenic coho salmon. J Fish Biol, 2003, 63: 806–823 10.1046/j.1095-8649.2003.00194.x
Devlin R H, Sundström L F, Muir W M. Interface of biotechnology and ecology for environmental risk assessments of transgenic fish. Trends Biotechnol, 2006, 24: 89–97 16380181, 10.1016/j.tibtech.2005.12.008, 1:CAS:528:DC%2BD28XhtV2lsrs%3D
Liu S J, Liu Y, Zhou G J, et al. The formation of tetraploid stocks of red crucian carp×common carp hybrids as an effect of interspecific hybridization. Aquaculture, 2001, 192: 171–186 10.1016/S0044-8486(00)00451-8
Liu S J, Hu F, Zhou G J, et al. Gonadal structure of triploid crucian carp produced by crossing allotetraploid hybrids of Carassius auratus red var. (♀) × Cyprinus carpio (♂) with Japanese crucian carp (Carassius auratus T. ETS). Acta Hydrobiol Sin, 2000, 24: 301–306
Feng H, Chen J, Luo J, et al. Cloning of Black Carp β-actin gene and primarily detecting the function of its promoter region. Acta Genet Sin, 2006, 33: 133–140 16529297, 10.1016/S0379-4172(06)60032-2, 1:CAS:528:DC%2BD2sXjs1Wmsbw%3D
Feng H, Zeng Z Q, Cheng J, et al. Molecular cloning and sequencing of growth hormone gene for black carp. Acta Laser Biol Sin, 2002, 11: 407–411 1:CAS:528:DC%2BD3sXosFKrsb0%3D
Feng H, Zeng Z Q, Liu S J, et al. Studies of F1 of transgenic allotraploid hybrids of Carassius auratus red var. (♀) × Cyprinus carpio (♂). Acta Genet Sin, 2002, 29: 434–437 12043572, 1:CAS:528:DC%2BD2cXktVSrsLc%3D
Feng H, Cheng J, Liu Y, et al. In Vitro expression and antibody preparation of black carp (Mylopharyngodon piceus) GH. Hereditas (Beijing), 2005, 27: 729–734 1:CAS:528:DC%2BD28XhtFejt7zL
Devlin R H, Biagi C A, Yesaki T Y, et al. Growth of domesticated transgenic fish. Nature, 2001, 409: 781–782 11236982, 10.1038/35057314, 1:CAS:528:DC%2BD3MXhsFCjsLc%3D
Wang Y P, Hu W, Wu G, et al. Genetic analysis of “all-fish” growth hormone gene transferred carp (Cyprinus carpio L.) and its F1 generation. Chin Sci Bull, 2001, 46: 1174–1177 1:CAS:528:DC%2BD3MXmtVSqsrY%3D
Author information
Authors and Affiliations
Corresponding author
Additional information
This article is published with open access at Springerlink.com
Rights and permissions
Open Access This is an open access article distributed under the terms of the Creative Commons Attribution Noncommercial License (https://creativecommons.org/licenses/by-nc/2.0), which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
About this article
Cite this article
Feng, H., Fu, Y., Luo, J. et al. Black carp growth hormone gene transgenic allotetraploid hybrids of Carassius auratus red var. (♀)×Cyprinus carpio (♂). Sci. China Life Sci. 54, 822–827 (2011). https://doi.org/10.1007/s11427-011-4210-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11427-011-4210-x