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Treatment of established colon carcinoma-bearing mice by dendritic cells pulsed with lysates of heat-treated tumor cells

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Abstract

To investigate the therapeutic effect of dendritic cells pulsed with lysates of heat-treated CT26 colon carcinoma cells. Bone marrow-derived DCs were pulsed with lysates of heat-treated tumor cells and were used to immunize BALB/c mice with established colon carcinoma. Cytotoxic T lymphocyte (CTL) response was detected. The therapeutic effect induced by DCs was observed by tumor weight and survival time. DCs pulsed with lysates of heat-treated tumor cells markedly induced specific cytotoxic activity of CTLs. Tumor growth in the immunized BALB/c mice was significantly inhibited and the survival time of the tumor-bearing mice was prolonged. DCs pulsed with lysates of heat-treated tumor cells have an observable therapeutic effect on established colon carcinoma-bearing mice.

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Authors and Affiliations

  1. Abdominal Surgery, Fujian Provincial Tumor Hospital, Teaching Hospital of Fujian Medical University, Fuzhou, 350014, China

    MinGang Ying & Sheng Liu

  2. Surgical Laboratory, Fujian Provincial Tumor Hospital, Teaching Hospital of Fujian Medical University, Fuzhou, 350014, China

    QiuHong Zhen, FuSheng Gong & YunQing Xie

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  1. MinGang Ying
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  2. QiuHong Zhen
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  3. Sheng Liu
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  4. FuSheng Gong
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  5. YunQing Xie
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Correspondence to MinGang Ying.

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Supported by the Key Project of Fujian Provincial Scientific Program (Grant No. 2007Y0014)

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Ying, M., Zhen, Q., Liu, S. et al. Treatment of established colon carcinoma-bearing mice by dendritic cells pulsed with lysates of heat-treated tumor cells. SCI CHINA SER C 52, 831–835 (2009). https://doi.org/10.1007/s11427-009-0112-6

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  • DOI: https://doi.org/10.1007/s11427-009-0112-6

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