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Gene analysis of an antiviral protein SP-2 from Chinese wild silkworm, Bombyx mandarina Moore and its bioactivity assay

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Abstract

The cDNA encoding an antiviral protein SP-2 against BmNPV was cloned from the midgut of Chinese wild silkworm, Bombyx mandarina Moore (GenBank access AY945210) based on the available information of the domesticated silkworm. Its cDNA was 855 bp encoding 284 amino acids with predicted molecular weight of 29.6 kDa. Its full length in genomics was 1376 bp, including 5 exons and 4 introns. The expression analysis indicated that it was only expressed in midgut, and its expression level was higher during feeding stage of larval instars while very lower during the moltism and mature stages. The deduced amino acid sequence of this protein showed eight-amino-acid variation compared with the counterpart of domesticated silkworm. Its antiviral activity was assayed through in vitro test. The results indicated that it showed strong bioactivity against BmNPV, and its activity was 1.6 fold higher that the counterpart of domesticated silkworm.

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Authors and Affiliations

  1. College of Animal Science, Zhejiang University, Hangzhou, 310029, China

    HuiPeng Yao, FangQing He, AiQin Guo, CuiPing Cao, XingMeng Lu & XiaoFeng Wu

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  1. HuiPeng Yao
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  2. FangQing He
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  3. AiQin Guo
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  4. CuiPing Cao
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  5. XingMeng Lu
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  6. XiaoFeng Wu
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Corresponding author

Correspondence to XiaoFeng Wu.

Additional information

Supported by the National High-tech Research Program (863 Program) of China (Grant No. 2007AA10Z159), the State Key Basic Research Program (973 Program) of China (Grant No. 2005CB121003), and the New-Century Training Program Foundation for the Talents of the Ministry of Education of China (Grant No. NCET-06-0524)

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Yao, H., He, F., Guo, A. et al. Gene analysis of an antiviral protein SP-2 from Chinese wild silkworm, Bombyx mandarina Moore and its bioactivity assay. SCI CHINA SER C 51, 879–884 (2008). https://doi.org/10.1007/s11427-008-0123-8

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  • DOI: https://doi.org/10.1007/s11427-008-0123-8

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