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The antibody preparation and expression of human Pescadillo

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Abstract

To explore the biological roles of human Pescadillo and investigate its potential effect on tumorigenesis, the cDNA of Pescadillo was fused with that of GST. After purification and elution, the purified GST-Pescadillo fusion protein was obtained, and the antibody against the fusion protein was generated. Endogenous Pescadillo protein was observed to be remarkably induced by estrogen. It was mainly distributed in the tissues such as breast, ovary and intestine, all of which contain proliferating cells, and was also detected in many cell lines of human cancer: renal carcinoma, hepatoma, ovarian cancer, colon carcinoma, and breast cancer. The expression level of Pescadillo was increased significantly in breast cancer tissues compared with their paired margin tissues. Taken together, these data suggest that Pescadillo may play important roles in the initiation and development of cancer and may be a potential target in cancer diagnosis and therapy.

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References

  1. Allende M L, Amsterdam A, Becker T, et al. Insertional mutagenesis in zebrafish identifies two novel genes, pescadillo and dead eye, essential for embryonic development. Genes Dev, 1996, 10(24): 3141–3155

    Article  PubMed  CAS  Google Scholar 

  2. Maiorana A, Tu X, Cheng G, et al. Role of pescadillo in the transformation and immortalization of mammalian cells. Oncogene, 2004, 23(42): 7116–7124

    Article  PubMed  CAS  Google Scholar 

  3. Monteiro A N, August A, Hanafusa H. Evidence for a transcriptional activation function of BRCA1 C-terminal region. Proc Natl Acad Sci USA, 1996, 93(24): 13595–13599

    Article  PubMed  CAS  Google Scholar 

  4. Callebaut I, Mornon J P. From BRCA1 to RAP1: A widespread BRCT module closely associated with DNA repair. FEBS Lett, 1997, 400(1): 25–30

    Article  PubMed  CAS  Google Scholar 

  5. Gowen L C, Avrutskaya A V, Latour A M, et al. BRCA1 required for transcription-coupled repair of oxidative DNA damage. Science, 1998, 281(5379): 1009–1012

    Article  PubMed  CAS  Google Scholar 

  6. Bork P, Hofmann K, Bucher P A. Superfamily of conserved domains in DNA damage-responsive cell cycle checkpoint proteins. FASEB J, 1997, 11(1): 68–76

    PubMed  CAS  Google Scholar 

  7. Zhang H, Fang Y, Huang C F, et al. Human Pescadiollo induces large-scale chromatin unfolding. Sci China C-Life Sci, 2005, 48(3): 270–276

    Article  PubMed  CAS  Google Scholar 

  8. Charpentier A H, Bednarek A K, Daniel R L, et al. Effects of estrogen on global gene expression: Identification of novel targets of estrogen action. Cancer Res, 2000, 60(21): 5977–5983

    PubMed  CAS  Google Scholar 

  9. Kinoshita Y, Jarell A D, Flaman J M, et al. Pescadillo, a novel cell cycle regulatory protein abnormally expressed in malignant cells. J Biol Chem, 2001, 276(9): 6656–6665

    Article  PubMed  CAS  Google Scholar 

  10. Liu Y F, Ding L H, Hao C F, et al. Expression and purification of XBP-1 fusion protein and preparation of polyclonal antibody against protein. Lett Biotechn, 2004, 20(6): 762–767

    CAS  Google Scholar 

  11. Zhao X R, Wang C X, Luo F J, et al. The Epstein-Barr virus encoded latent membrane protein 1(EBV-LMP1) activates expression of Cyclin D1. Prog Biochem Biophy, 2001, 28(5): 704–710

    Google Scholar 

  12. Haque J, Boger S, Li J, et al. The murine Pes1 gene encodes a nuclear protein containing a BRCT domain. Genomics, 2000, 70(2): 201–210

    Article  PubMed  CAS  Google Scholar 

  13. Lerch-Gaggl A, Haque J, Li J, et al. Pescadillo is essential for nucleolar assembly, ribosome biogenesis, and mammalian cell proliferation. J Biol Chem, 2002, 277(47): 45347–45355

    Article  PubMed  CAS  Google Scholar 

  14. Prisco M, Maiorana A, Guerzoni C et al. Role of pescadillo and upstream binding factor in the proliferation and differentiation of murine myeloid cells. Mol Cell Biol, 2004, 24(12): 5421–5433

    Article  PubMed  CAS  Google Scholar 

  15. Metivier R, Penot G, Hubner M R, et al. Estrogen receptor-alpha directs ordered, cyclical, and combinatorial recruitment of cofactors on a natural target promoter. Cell, 2003, 115(6): 751–763

    Article  PubMed  CAS  Google Scholar 

  16. Pearce S T, Jordan V C. The biological role of estrogen receptors alpha and beta in cancer. Crit Rev Oncol Hematol, 2004, 50(1): 3–22

    Article  PubMed  Google Scholar 

  17. Nilsson S, Makela S, Treuter E, et al. Mechanisms of estrogen action. Physiol Rev, 2001, 81(4): 1535–1565

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Beijing Institute of Biotechnology, Beijing, 100850, China

    Zhang Hao, Li JiePing, Wang XiaoHui, Sun Yan, Yuan Bin, Yang ZhiHong, Jiang YanChao, Zeng Min, Ding LiHua, Ning Kang, Li JieZhi, Huang CuiFen & Ye QiNong

  2. Cancer Division, First Affiliated Hospital of the General Hospital of PLA, Beijing, 100373, China

    Zhu JianHua

  3. Pathology Division, General Hospital of PLA, Beijing, 100373, China

    Liu AiJun

Authors
  1. Zhang Hao
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  2. Li JiePing
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  3. Wang XiaoHui
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  4. Sun Yan
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  5. Yuan Bin
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  6. Yang ZhiHong
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  7. Jiang YanChao
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  8. Zeng Min
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  9. Ding LiHua
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  10. Ning Kang
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  11. Zhu JianHua
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  12. Li JieZhi
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  13. Huang CuiFen
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  14. Liu AiJun
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  15. Ye QiNong
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Corresponding authors

Correspondence to Liu AiJun or Ye QiNong.

Additional information

Supported by the National Natural Science Foundation of China (Grant Nos. 30500191, 30530320, 30470378, and 30625035)

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Zhang, H., Li, J., Wang, X. et al. The antibody preparation and expression of human Pescadillo. SCI CHINA SER C 50, 298–304 (2007). https://doi.org/10.1007/s11427-007-0045-x

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  • DOI: https://doi.org/10.1007/s11427-007-0045-x

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