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Establishment of the diploid gynogenetic hybrid clonal line of red crucian carp × common carp

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Abstract

This study investigated the gynogenetic cytobiological behavior of the third gynogenetic generation (G3), which was generated from the diploid eggs produced by the second gynogenetic generation (G2) of red crucian carp × common carp, and determined the chromosomal numbers of G3, G2×scatter scale carp and G2×allotetraploid hybrids of red crucian carp × common carp. The results showed that the diploid eggs of G2 with 100 chromosomes, activated by UV-irradiated sperm from scatter scale carp and without the treatment for doubling the chromosomes, could develop into G3 with 100 chromosomes. Similar to the first and second gynogenetic generations (G1 and G2), G3 was also diploid (2n=100) and presented the hybrid traits. The triploids (3n=150) and tetraploids (4n=200) were produced by crossing G2 with scatter scale carp, and crossing G2 with allotetraploids, respectively. The extrusion of the second polar body in the eggs of G2 ruled out the possibility that the retention of the second polar body led to the formation of the diploid eggs. In addition, we discussed the mechanism of the formation of the diploid eggs generated by G2. The establishment of the diploid gynogenesis clonal line (G1, G2 and G3) provided the evidence that the diploid eggs were able to develop into a new diploid hybrid clonal line by gynogenesis. By producing the diploid eggs as a unique reproductive way, the diploid gynogenetic progeny of allotetraploid hybrids of red crucian carp × common carp had important significances in both biological evolution and production application.

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Authors and Affiliations

  1. Key Laboratory of Protein Chemistry and Fish Developmental Biology of Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha, 410081, China

    Liu ShaoJun, Duan Wei, Tao Min, Zhang Chun, Sun YuanDong, Shen JiaMin, Wang Jing, Luo KaiKun & Liu Yun

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  1. Liu ShaoJun
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  2. Duan Wei
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  3. Tao Min
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  4. Zhang Chun
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  5. Sun YuanDong
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  6. Shen JiaMin
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  7. Wang Jing
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  8. Luo KaiKun
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  9. Liu Yun
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Corresponding author

Correspondence to Liu ShaoJun.

Additional information

Supported by the National Natural Science Foundation of China (Grant Nos. 30330480 and 30571444), the Doctoral Station of University of the Education Ministry of China (Grant No. 200405422001), and the Agriculture Technological Production Foundation of the Ministry of Science and Technology of China (Grant No. 2005380)

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Liu, S., Duan, W., Tao, M. et al. Establishment of the diploid gynogenetic hybrid clonal line of red crucian carp × common carp. SCI CHINA SER C 50, 186–193 (2007). https://doi.org/10.1007/s11427-007-0032-2

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  • DOI: https://doi.org/10.1007/s11427-007-0032-2

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