Abstract
The genomic DNA extracted from chicken embryo fibroblast (CEF) infected with a Chinese field isolate HA9901 of reticuloendotheliosis virus (REV) was used as the template to amplify the REV proviral genomic cDNA by PCR with 6 pairs of primers according to published sequences. Six overlapping fragments were amplified, cloned into the TA vector and sequenced, including a fragment which was amplified from the circular proviral cDNA and covering both 5′-and 3′-ends. The complete sequence of the whole genome was established and analyzed with a DNAstar software. Comparisons of the sequence with two other strains demonstrated that the genomes of REV were relatively conservative, the homogenecity for all genes or LTR fragments of the 3 strains was over 92%, no matter whether they were isolated from different species and regions in different years. But, the homology of Chinese strain HA9901 to a fowl pox virus-associated strain from Chickens was higher than that to strain SNV isolated from ducks.
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References
Walker, M. H, Rup, B. J., Rubin, A. S. et al., Specificity in the immunosuppression induced by avian reticuloendotheliosis virus, Infect Immun., 1983, 40: 225–235.
Witter, R. L., Reticuloendotheliosis, in Disease of Poultry (ed. Calnek, B. W.), 10th ed., Ames: Iowa University Press, 467–484.
Ridgway, A. A., Swift, R. A., Kung, H. J. et al., In vitro transcription analysis of the viral promoter involved in c-myc activation in chicken lymphomas: Detection and mapping for two RNA initiation sites with the reticuloendotheliosis virus long terminal repeat, J. Virol., 1985, 54: 161–170.
Shimotohno, K., Mizutani, S., Temin, H. M., Sequence of retrovirus provirus resembles that of bacterial transposable elements, Nature, 1980, 285: 550–554.
Gabus-Darlix, C., Darlix, J., Novel internal ribosome entry site and vector containing same, GenBank, GI: 6732807.
Weaver, T. A., Talbot, K. J., Panganiban A. T., Spleen necosis virus gag polyprotein is necessary for partical assembly and release but not for proteolytic processing, J. Virol., 1990, 64: 2642–2652.
Wilhelmsen, L. C., Eggletin, K., Temin, H. M., Nucleic acid sequences of the oncogene v-rel in reticuloendotheliosis virus strain T and its cellular homolog the proto-oncogene c-rel, J. Virol., 1984, 52: 172–182.
Panganiban, A. T., Temin, H. M., Circles with two tandem LTRs are precursors to integrated retrovirus DNA, Cell, 1984, 36(3): 673–679.
Singh, P., Kim, T. J., Tripathy, D. N., Re-emerging fowpox: Evaluation of isolates from vaccinated flocks, Avian Pathol., 2000, 29: 449–455.
Zhang, Z., Cui, Z., Jiang, S. et al., Co-infection with Marek’s disease virus and reticuloendotheliosis virus in tumor samples of chicken, Chin. J. Prevent. Vet. Med. (in Chinese), 2003, 4: 274–278.
Zhang, Z., Cui, Z., Isolation and identification of reticuloendotheliosis vrus from tumors infected with subgroup J avian leukosis virus, Chin. J. Vet. Sci. (in Chinese), 2004, 1: 10–13.
Zhang, Z., Cui, Z., Isolation of recombinant field strains of Marek’s disease virus integrated with reticuloendotheliosis virus genome fragments, Science in China, Ser. C, 2005, 48(1): 81–88.
Cui, Z., Du, Y., Zhao, W., Reticuloendotheliosis virus infection and immunodepression of chicken flocks, Chin. J. Vet. Sci., 2000, 34(4): 1–3.
Cui, Z., Lee, L. F., Silva, R. F. et al., Monoclonal antibodies against avian reticuloendotheliosis virus’ identification of strain-specific and strain-common epitopes, J. Immunol., 1986, 136: 4237–4242.
Ridgway, A. A., Swift, R. A., Kung, H. J. et al., In vitro transcription analysis of the viral promoter involved in c-myc activation in chicken B lymphomas: Detection and mapping of two RNA initiation sites within the reticuloendotheliosis virus long terminal repeat, J. Virol., 1985, 54(1): 161–170.
Chen, I. S., Malk, T. W., O’Pear, J. J. et al., Characterization of reticuloendotheliosis virus strain T DNA and isolation of a novel variant of reticuloendotheliosis virus strain T by molecular cloning, J. Virol., 1981, 40: 800–811.
Trager, W., A new virus of ducks interfering with development of malaria parasite (Plasmodium lophurase), Proc. Soc. Exp. Biol. Med., 1959, 101: 578–582.
Wilhelmsen, K. C., Eggleton, K., Temin, H. M., Nucleic acid sequences of the oncogene v-rel in reticuloendotheliosis virus strain T and its cellular homolog, the proto-oncogene c-rel, J. Virol, 1984, 52(1): 172–182.
Zhao, W., Ding, J., Cui, Z., Analysis of LTR sequence from different REV strains, Journal of Shanghai Agricultural College (in Chinese), 2001, 1(3): 18–21.
Kost, R. V., Jones, D., Isflrt, R. J. et al., Retrovirus insertion into herpesvirus: Characterization of a Marek’s disease virus harboring a solo LTR, Virology, 1992, 192: 161–169.
Heritig, C., Coupar, B. E., Gould, A. R. et al., Field and vaccine strains of fowlpox carry integrated sequences from the avian retrovirus, reticuloendotheliosis virus, Virology, 1997, 235: 367–376.
Isfort, R. J., Jones, D., Kost, R. G. et al., Retrovirus insertion into herpesvirus in vitro and in vivo, Proc. Natl. Acad. Sci., USA, 1992, 89: 991–995.
Garcia, M., Narang, N., Reed, W. M. et al., Molecular characterization of reticuloendotheliosis virus insertions in the genome of field and vaccine strains of fowl poxvirus, Avian Dis., 2003, 47: 343–354.
Singh, P., Schitzlein, W. M., Tripathy, D. N., Reticuloendotheliosis virus sequences within the genomes of field strains of fowlpox virus display variability, J. Virol, 2003, 77(10): 5855–5862.
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Wang, Y., Cui, Z. & Jiang, S. Sequence analysis for the complete proviral genome of reticuloendotheliosis virus Chinese strain HA9901. SCI CHINA SER C 49, 149–157 (2006). https://doi.org/10.1007/s11427-006-0149-8
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DOI: https://doi.org/10.1007/s11427-006-0149-8