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Electrogenerated chemiluminescence aptasensor for thrombin incorporating poly(pyrrole-co-pyrrole propylic acid) nanoparticles loaded with aptamer and ruthenium complex

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Abstract

A novel electrogenerated chemiluminescence (ECL) aptasensor for highly sensitive detection of thrombin was developed on the basis of poly(pyrrole-co-pyrrole propylic acid) nanoparticles loaded with aptamer and ruthenium complex. Thrombin binding aptamers served as the molecular recognition elements and ruthenium bis(2,2′-bipyridine) (2,2′-bipyridine-4,4′-dicarboxylic acid)-ethylenediamine (Ru1) was used as an ECL signal complex. Novel electroactive polymers poly(pyrrole-co-pyrrole propylic acid) nanoparticles (Ppy-pa NPs) were synthesized by a simple alcohol-assisted microemulsion polymerization. Ru1-Ppy-pa NPs were synthesized by covalently coupling Ru1 with the Ppy-pa NPs. Ppy-pa NPs and Ru1-Ppy-pa NPs were characterized using a fourier transform infrared spectrometer, super-conducting fourier digital NMR spectrometer, and transmission electron microscope. One ECL chemical sensor fabricated by immobilizing the Ru1-Ppy-pa NPs on PIGE was developed for the determination of TprA with a high sensitivity and stability. The ECL aptasensor was fabricated by covalently coupling the thrombin binding aptamer-I (TBA-I) onto the surface of the paraffin-impregnated graphite electrode, which had been covalently modified with a monolayer of 4-aminobenzene sulfonic acid via electrochemical oxidations, for capturing thrombin onto the electrode and then the TBA-II labeled with Ru1-Ppy-pa NPs was bound with epitope of thrombin. The ECL aptasensor showed an extremely low detection limit of 3.0×l0−16 mol/L for thrombin and a good selectivity. This work demonstrated that using Ppy-pa NPs as a carrier of ruthenium complex and molecular recognition element was a promising approach for the fabrication of ECL biosensor with high sensitivity.

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Authors and Affiliations

  1. Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education; School of Chemistry and Materials Science, Shaanxi Normal University, Xi’an, 710062, China

    Fen Ma, LiJuan Jia, Yu Zhang, Bo Sun, HongLan Qi, Qiang Gao & ChengXiao Zhang

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  1. Fen Ma
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  2. LiJuan Jia
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  3. Yu Zhang
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  4. Bo Sun
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  5. HongLan Qi
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  6. Qiang Gao
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  7. ChengXiao Zhang
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Correspondence to ChengXiao Zhang.

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Ma, F., Jia, L., Zhang, Y. et al. Electrogenerated chemiluminescence aptasensor for thrombin incorporating poly(pyrrole-co-pyrrole propylic acid) nanoparticles loaded with aptamer and ruthenium complex. Sci. China Chem. 54, 1357–1364 (2011). https://doi.org/10.1007/s11426-011-4329-4

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  • DOI: https://doi.org/10.1007/s11426-011-4329-4

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