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Fluorescence polarization immunoassay for salinomycin based on monoclonal antibodies

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Abstract

A fluorescence polarization immunoassay (FPIA) for the determination of salinomycin (SAL) was developed by using anti-SAL monoclonal antibodies (mAb). Fluorescein labeled SAL (tracer) was synthesized by the N-hydroxysuccinimide active ester method and purified using thin layer chromatography (TLC). The developed FPIA for SAL had a dynamic range from 0.60 to 2193 ng/mL with an IC50 value of 33.2 ng/mL and a detection limit (LOD) of 0.08 ng/mL. No significant cross-reactivities were observed with other drugs but 67.6% with narasin.

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Authors and Affiliations

  1. National Ference Laboratories for Veterinary Drug Residue; College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China

    ZhanHui Wang, LinLi Cheng, WeiMin Shi, SuXia Zhang & JianZhong Shen

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  1. ZhanHui Wang
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  2. LinLi Cheng
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  3. WeiMin Shi
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  4. SuXia Zhang
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  5. JianZhong Shen
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Correspondence to JianZhong Shen.

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Wang, Z., Cheng, L., Shi, W. et al. Fluorescence polarization immunoassay for salinomycin based on monoclonal antibodies. Sci. China Chem. 53, 553–555 (2010). https://doi.org/10.1007/s11426-010-0085-0

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  • DOI: https://doi.org/10.1007/s11426-010-0085-0

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