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Nano Au/TiO2 hollow microsphere membranes for the improved sensitivity of detecting specific DNA sequences related to transgenes in transgenic plants

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Abstract

Gold nanoparticles (nano Au)/titanium dioxide (TiO2) hollow microsphere membranes were prepared on the carbon paste electrode (CPE) for enhancing the sensitivity of DNA hybridization detection. The immobilization of nano Au and TiO2 microsphere was investigated with cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The hybridization events were monitored with EIS using [Fe(CN)6]3−/4− as indicator. The sequence-specific DNA of the 35S promoter from cauliflower mosaic virus (CaMV35S) gene was detected with this DNA electrochemical sensor. The dynamic detection range was from 1.0×10−12 to 1.0×10−8 mol/L DNA and a detection limit of 2.3×10−13 mol/L could be obtained. The polymerase chain reaction (PCR) amplification of the terminator of nopaline synthase (NOS) gene from the real sample of a kind of transgenic soybean was also satisfactorily detected.

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Authors and Affiliations

  1. College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China

    YongChun Zhang, Tao Yang, Na Zhou, Wei Zhang & Kui Jiao

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  1. YongChun Zhang
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  2. Tao Yang
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  3. Na Zhou
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  4. Wei Zhang
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  5. Kui Jiao
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Correspondence to Kui Jiao.

Additional information

Supported by the National Natural Science Foundation of China (Grant Nos. 20635020 and 20375020), Doctoral Foundation of the Ministry of Education of China (Grant No. 20060426001) and Natural Science Foundation of Qingdao City (Grant No. 04-2-JZP-8)

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Zhang, Y., Yang, T., Zhou, N. et al. Nano Au/TiO2 hollow microsphere membranes for the improved sensitivity of detecting specific DNA sequences related to transgenes in transgenic plants. Sci. China Ser. B-Chem. 51, 1066–1073 (2008). https://doi.org/10.1007/s11426-008-0116-2

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  • DOI: https://doi.org/10.1007/s11426-008-0116-2

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