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Determination of carcinoembryonic antigen using a novel amperometric enzyme-electrode based on layer-by-layer assembly of gold nanoparticles and thionine

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Abstract

Electrochemical sensing of carcinoembryonic antigen (CEA) on a gold electrode modified by the sequential incorporation of the mediator, thionine (Thi), and gold nanoparticles (nano-Au), through covalent linkage and electrostatic interactions onto a self-assembled monolayer configuration is described in this paper. The enzyme, horseradish peroxidase (HRP), was employed to block the possible remaining active sites of the nano-Au monolayer, avoid the non-specific adsorption, instead of bovine serum albumin (BSA), and amplify the response of the antigen-antibody reaction. Electrochemical experiments indicated highly efficient electron transfer by the imbedded Thi mediator and adsorbed nano-Au. The HRP kept its activity after immobilization, and the studied electrode showed sensitive response to CEA and high stability during a long period of storage. The working range for the system was 2.5 to 80.0 ng/mL with a detection limit of 0.90 ng/mL. The model membrane system in this work is a potential biosensor for mimicking the other immunosensor and enzyme sensor.

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Authors and Affiliations

  1. Chongqing Key Laboratory of Analytical Chemistry, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, China

    Yuan Ruo, Zhuo Ying, Chai YaQin, Zhang Ying & Sun AiLi

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  1. Yuan Ruo
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  2. Zhuo Ying
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  3. Chai YaQin
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  4. Zhang Ying
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  5. Sun AiLi
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Correspondence to Yuan Ruo.

Additional information

Supported by the National Natural Science Foundation of China (Grant No. 20675064), the Natural Science Foundation of Chongqing City (Grant Nos. CSTC-2004BB4149 and 2005BB4100) and the High Technology Project Foundation of Southwest University (XSGX02), China

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Yuan, R., Zhuo, Y., Chai, Y. et al. Determination of carcinoembryonic antigen using a novel amperometric enzyme-electrode based on layer-by-layer assembly of gold nanoparticles and thionine. SCI CHINA SER B 50, 97–104 (2007). https://doi.org/10.1007/s11426-007-0017-9

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  • DOI: https://doi.org/10.1007/s11426-007-0017-9

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