Abstract
Four major phytocannabinoids (∆8-tetrahydrocannabinol, ∆9-tetrahydrocannabinol, cannabidiol, and cannabinol, 100 mg each) were dissolved in Duquenois reagent containing vanillin and acetaldehyde in ethanol, and concentrated hydrochloric acid was added. The chromophores derived from the four cannabinoids were characterized by mass spectrometry after dichloromethane extraction and partial purification by Florisil column chromatography with dichloromethane-ethanol solutions, using a unique replacement technique, which caused various modes of peak shifts in mass spectra giving information for structural elucidation. It was disclosed that there were three types of condensates, among which the structures of two condensates could be delineated, but one condensate remains to be clarified. To our knowledge, this is the first report to simultaneously propose the structures of the Duquenois reaction chromophores for the four major phytocannabinoids.
References
Duquenois P, Negm H M (1938) Identification et dosage de cannabis indica. J Egypt Med Assoc 21:224–227
Duquenois P, Negm H M (1938) Contribution and identification et au dosage du hashish. Annal Med Legale 18:485–506
Hughes RB, Warner JV (1978) A study of false positives in the chemical identification of marihuana. J Forensic Sci 23:304–310
Bailey K (1979) The value of the Duquenois test for cannabis–a survey. J Forensic Sci 24:817–841
Butler WO (1962) Duquenois-Levine test for marihuana. J Assoc Off Agr Chem 45:597–599
Frederick W, Fochtman MS, Charles LW (1971) A note on the Duquenois-Levine test from marijuana. Clin Pharmacol 4:287–289
Kovar K-A, Keck M (1988) Zur Kenntnis der Duquenois-Reaktion auf Haschisch und Marihuana. Arch Pharm 321:249–252
Pitt CG, Hendron RW, Hsia RS (1972) The specificity of the Duquenois color test for marihuana and hashish. J Forensic Sci 17:693–700
Jacobs AD, Steiner RR (2014) Detection of the Duquenois-Levine chromophore in a marijuana sample. Forensic Sci Int 239:1–5
Aramaki H, Tomiyasu N, Yoshimura H, Tsukamoto H (1968) Forensic chemical studies on marihuana 1. Detection method of the principal constituents by thin-layer and gas chromatographies. Chem Pharm Bull 16:822–826
Gaoni Y, Mechoulam R (1966) Hashish-VII: the isomerization of cannabidiol to tetrahydrocannabinols. Tetrahedron 22:1481–1488
Yoshimura H, Watanabe K, Oguri K, Fujiwara M, Ueki S (1978) Synthesis and pharmacological activity of a phosphate ester of ∆8-tetrahydrocannabinol. J Med Chem 21:1079–1081
Watanabe K, Miyamoto M, Yamaori S, Hasegawa K, Watanabe K, Suzuki O (2013) Human brain microsomes: their abilities to metabolize tetrahydrocannabinols and cannabinol. Forensic Toxicol 31:307–311
Acknowledgments
A part of this work was supported by the Specific Research Fund of Hokuriku University.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
There are no financial relations that could lead to a conflict of interest.
Ethical approval
This article does not contain any studies with human participants or animals performed by any of the authors.
Rights and permissions
About this article
Cite this article
Watanabe, K., Honda, G., Miyagi, T. et al. The Duquenois reaction revisited: mass spectrometric estimation of chromophore structures derived from major phytocannabinoids. Forensic Toxicol 35, 185–189 (2017). https://doi.org/10.1007/s11419-016-0337-6
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11419-016-0337-6