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Constituents from Vitex negundo var. heterophylla and their inhibition of nitric oxide production

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Abstract

An iridoid glucoside, 10-p-hydroxybenzoyl-6β-hydroxyiridoid 1-O-β-d-(6′-O-p-hydroxybenzoyl)-glucopyranoside (1), and a phenol glucoside, 4-hydroxyphenethanol 3-O-β-d-(6′-O-p-hydroxybenzoyl)-glucopyranoside (2), along with nine known compounds (3–11) were isolated from the dried leaves of Vitex negundo var. heterophylla. Their structures were elucidated by extensive analysis of NMR spectra. All of the isolated compounds were evaluated for their inhibitory effects on nitric oxide production in RAW 264.7 macrophages. Compounds 2 and 3 exhibited obvious inhibitory effects on NO production with IC50 values of 30.76 and 49.89 μM, respectively. Molecular docking studies of compounds 2 and 3 with nitric oxide synthase (NOS) further confirmed the above results.

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Acknowledgments

This work was financially supported by grants from Key Projects of the National Science and Technology Pillar Program (Grant No. 2012BAI30B02) and Fund of the Educational Department of Liaoning Province (Grant No. L2011177).

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Authors and Affiliations

  1. Key Laboratory of Structure-Based Drug Design and Discovery, Department of Natural Products Chemistry, School of Traditional Chinese Materia Medica, Ministry of Education, Shenyang Pharmaceutical University, Shenyang, 110016, China

    Chongyue Qiu, Liang Tong, Ting Yuan, Fei Wang & Lixia Chen

  2. School of Pharmacy, Yantai University, Yantai, 264005, China

    Feng Zhao

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  1. Chongyue Qiu
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  2. Liang Tong
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  3. Ting Yuan
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  4. Fei Wang
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  5. Feng Zhao
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  6. Lixia Chen
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Correspondence to Lixia Chen.

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Qiu, C., Tong, L., Yuan, T. et al. Constituents from Vitex negundo var. heterophylla and their inhibition of nitric oxide production. J Nat Med 71, 292–298 (2017). https://doi.org/10.1007/s11418-016-1032-y

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  • DOI: https://doi.org/10.1007/s11418-016-1032-y

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