Abstract
Immunogen quality is one important factor that contributes to desirable antibody characteristics. Highly specific antibodies against miroestrol can be used to develop a quality control immunoassay for Pueraria candollei products. In this study, we investigated how various immunogen preparations affect antibody properties. The results show that immunogen prepared using the Mannich reaction provides antibodies with higher specificity and sensitivity against miroestrol than immunogen prepared with the periodate reaction. The results suggest the Mannich reaction maintains the original structure of miroestrol and generates useful antibodies for developing immunoassays.
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Acknowledgments
The authors thank Dr. Chaiyo Chaichantipyuth, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Thailand for the gift of authentic standard of miroestrol. We acknowledge the support of the Royal Golden Jubilee PhD program by Thailand Research Fund organization (Grant no. PHD/0050/2556).
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Kitisripanya, T., Jutathis, K., Inyai, C. et al. Anti-miroestrol polyclonal antibodies: a comparison of immunogen preparations used to obtain desired antibody properties. J Nat Med 70, 296–299 (2016). https://doi.org/10.1007/s11418-015-0949-x
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DOI: https://doi.org/10.1007/s11418-015-0949-x