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Phytochemical analysis and effects of Pteris vittata extract on visual processes

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Abstract

The present study was designed to explore the possible effects of Pteris vittata on visual sensitivity, ERG waves, and other components of the visual system. Electrophysiological techniques including electroretinography (ERG) were used in the present study. The phytochemical composition of the extract was investigated using liquid chromatography–mass spectrometry (LC–MS) techniques. The results indicated that the extract significantly augmented dark- and light-adapted ERG b-wave amplitude. Furthermore, these findings showed that P. vittata extract does not have Gamma-aminobutyric acid receptor antagonistic activity but may function as a retinal neural antagonist in bullfrog retina. P. vittata extract improved the visual sensitivity by 0.8 log unit of light intensity, and reduced the regeneration time for rhodopsin. The six main peaks obtained through LC–MS were identified as flavonoids. Based on these results, it was concluded that P. vittata extract or its constituents may be used to treat eye diseases.

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References

  1. Singh M, Govindarajan R, Rawat AKS, Khare PB (2008) Antimicrobial flavonoid rutin from Pteris vittata L. against pathogenic gastrointestinal microflora. Am Fern J 98:98–103

    Article  Google Scholar 

  2. Benjamin A, Manickam VS (2007) Medicinal pteridophytes from the Western Ghats. Indian J Tradit Know 6:611–618

    Google Scholar 

  3. Upreti K, Jalal JS, Tewari LM, Joshi GC, Pangtey YPS, Tewari G (2009) Ethnomedicinal uses of Pteridophytes of Kumaun Himalaya, Uttarakhand. India. J Am Sci 5:167–170

    Google Scholar 

  4. Rout SD, Panda T, Mishra N (2009) Ethnomedicinal studies on some pteridophytes of Similipal Biosphere Reserve, Orissa, India. Int J Med Sci 1:192–197

    Google Scholar 

  5. Lai HY, Lim YY (2011) Evaluation of antioxidant activities of the methanolic extracts of selected ferns in Malaysia. Int J Environ Sci Develop 2:442–447

    Article  Google Scholar 

  6. Imperato F (2006) The new flavone ester apigenin 7-O-p-hydroxybenzoate and three di-C-glycosylflavones from Pteris vittata. Am Fern J 96:62–65

    Article  Google Scholar 

  7. Salatino MLF, Prado J (1998) Flavonoid glycosides of Pteridaceae from Brazil. Biochem Syst Ecol 26:761–769

    Article  CAS  Google Scholar 

  8. Carr RE, Siegel IM (1982) Analysis of the ERG components. In: Schachat WS (ed) Visual electrodiagnostic testing: a practical guide for the clinician. Williams and Wilkins, Baltimore, pp 9–11

    Google Scholar 

  9. Brown KT (1969) The electroretinogram: its components and their origins. UCLA Forum Med Sci 8:319–378

    CAS  PubMed  Google Scholar 

  10. Hejtmancik JF, FitzGibbon EJ, Caruso RC (2008) Vision. In: Conn PM (ed) Neuroscience in medicine, 3rd edn. Humana, New York, pp 531–574

    Chapter  Google Scholar 

  11. Stockton RA, Slaughter MM (1989) B-wave of the electroretinogram. A reflection of ON bipolar cell activity. J Gen Physiol 93:101–122

    Article  CAS  PubMed  Google Scholar 

  12. Wen R, Oakley B (1990) K (+)-evoked Muller cell depolarization generates b-wave of electroretinogram in toad retina. Proc Natl Acad Sci USA 87:2117–2121

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  13. Steinberg RH, Schmidt R, Brown KT (1970) Intracellular responses to light from cat pigment epithelium: origin of the electroretinogram c-wave. Nature 227:728–730

    Article  CAS  PubMed  Google Scholar 

  14. Dong CJ, Hare WA (2000) Contribution to the kinetics and amplitude of the electroretinogram b-wave by third-order retinal neurons in the rabbit retina. Vision Res 40:579–589

    Article  CAS  PubMed  Google Scholar 

  15. Linsenmeier RA, Mines AH, Steinberg RH (1983) Effects of hypoxia and hypercapnia on the light peak and electroretinogram of the cat. Invest Ophthalmol Vis Sci 24:37–46

    CAS  PubMed  Google Scholar 

  16. Wahid F, Jung H, Khan T, Hwang KH, Kim YY (2010) Effects of red ginseng extract on visual sensitivity and ERG b-wave of bullfrog’s eye. Planta Med 76:426–432

    Article  CAS  PubMed  Google Scholar 

  17. Wahid F, Khan T, Subhan F, Khan MA, Kim YY (2010) Ginseng pharmacology: multiple molecular targets and recent clinical trials. Drug Future 35:399–407

    Article  CAS  Google Scholar 

  18. Wahid F, Jung H, Khan T, Hwang KH, Park JS, Chang SC, Khan MA, Kim YY (2011) Effects of Rubus coreanus extract on visual processes in bullfrog’s eye. J Ethnopharmacol 138:333–339

    Article  PubMed  Google Scholar 

  19. Kupenova P, Popova E, Vitanova L (2008) GABAa and GABAc receptor mediated influences on the intensity-response functions of the b- and d-wave in the frog ERG. Vision Res 48:882–892

    Article  CAS  PubMed  Google Scholar 

  20. Wassle H, Koulen P, Brandstatter JH, Fletcher EL, Becker CM (1998) Glycine and GABA receptors in the mammalian retina. Vision Res 38:1411–1430

    Article  CAS  PubMed  Google Scholar 

  21. Arnarsson A, Eysteinsson T (1997) The role of GABA in modulating the Xenopus electroretinogram. Visual Neurosci 14:1143–1152

    Article  CAS  Google Scholar 

  22. Maeda T, Van Hooser JP, Driessen CA, Filipek S, Janssen JM, Palczewski K (2003) Evaluation of the role of the retinal G protein-coupled receptor (RGR) in the vertebrate retina in vivo. J Neurochem 85:944–956

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  23. Gattuso G, Barreca D, Gargiulli C, Leuzzi U, Caristi C (2007) Flavonoid composition of citrus juices. Molecules 12:1641–1673

    Article  CAS  PubMed  Google Scholar 

  24. Hossain MB, Rai DK, Brunton NP, Martin-Diana AB, Barry-Ryan C (2010) Characterization of phenolic composition in Lamiaceae spices by LC-ESI-MS/MS. J Agric Food Chem 58:10576–10581

    Article  CAS  PubMed  Google Scholar 

  25. Plazonic A, Bucar F, Males Z, Mornar A, Nigovic B, Kujundzic N (2009) Identification and quantification of flavonoids and phenolic acids in burr parsley (Caucalis platycarpos L.), using high-performance liquid Chromatography with diode array detection and electrospray ionization mass spectrometry. Molecules 14:2466–2490

    Article  CAS  PubMed  Google Scholar 

  26. Imperato F, Telesca A (2000) 6-C-β-Cellobiosylisoscutellarein-8-methyl ether, a new flavonoid from Pteris vittata. Am Fern J 90:42–45

    Article  Google Scholar 

  27. Wang HB, Wong MH, Lan CY, Qin YR, Shu WS, Qiu RL, Ye ZH (2010) Effect of arsenic on flavonoid contents in Pteris species. Biochem Syst Ecol 38:529–537

    Article  CAS  Google Scholar 

  28. Biel M, Seeliger M, Pfeifer A, Kohler K, Gerstner A, Ludwig A, Jaissle G, Fauser S, Zrenner E, Hofmann F (1999) Selective loss of cone function in mice lacking the cyclic nucleotide-gated channel CNG3. Proc Natl Acad Sci USA 96:7553–7557

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  29. Li L, Dowling JE (2000) Effects of dopamine depletion on visual sensitivity of zebrafish. J Neurosci 20:1893–1903

    CAS  PubMed  Google Scholar 

  30. Popova E, Mitova L, Vitanova L, Kupenova P (2003) Participation of the GABAergic system in the action of 2-amino-4-phosphonobutyrate on the OFF responses of frog retinal ganglion cells. Vision Res 43:607–616

    Article  CAS  PubMed  Google Scholar 

  31. Ostroy SE, Frede SM, Wagner EF, Gaitatzes CG, Janle EM (1994) Decreased rhodopsinregeneration in diabetic mouse eyes. Invest Ophthalmol Vis Sci 35:3905–3909

    CAS  PubMed  Google Scholar 

  32. Dorea JG, Olson JA (1986) The rate of rhodopsin regeneration in the bleached eyes of zinc-deficient rats in the dark. J Nutr 116:121–127

    CAS  PubMed  Google Scholar 

  33. Lamb TD, Pugh EN (2006) Phototransduction, dark adaptation, and rhodopsin regeneration. The Proctor lecture. Invest Ophthalmol Vis Sci 47:5137–5152

    Article  PubMed  Google Scholar 

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Acknowledgment

This work was supported by the Kyungpook National University research fund 2014.

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Authors and Affiliations

  1. Biotechnology Program, Department of Environmental Sciences, COMSATS Institute of Information Technology, Abbottabad, 22060, Pakistan

    Fazli Wahid

  2. Department of Pharmacy, COMSATS Institute of Information Technology, Abbottabad, 22060, Pakistan

    Taous Khan

  3. Department of Pharmacy, Abdul Wali Khan University, Mardan, Pakistan

    Omer Shehzad

  4. Department of Biomedical Engineering, National University of Sciences and Technology, Islamabad, Pakistan

    Adeeb Shehzad

  5. School of Life Sciences, College of Natural Sciences, Kyungpook National University, Daegu, 702-701, South Korea

    You Young Kim

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  1. Fazli Wahid
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  2. Taous Khan
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  4. Adeeb Shehzad
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Correspondence to Fazli Wahid or You Young Kim.

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Wahid, F., Khan, T., Shehzad, O. et al. Phytochemical analysis and effects of Pteris vittata extract on visual processes. J Nat Med 70, 8–17 (2016). https://doi.org/10.1007/s11418-015-0930-8

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  • DOI: https://doi.org/10.1007/s11418-015-0930-8

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