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Effect of phenylalanine on Taxol production and antioxidant activity of extracts of suspension-cultured hazel (Corylus avellana L.) cells

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Abstract

Taxol is produced by a few microorganisms and plants such as yew (Taxus sp.). Recent researches have shown that hazel (Corylus avellana L.) is also able to produce Taxol. In the present study, effects of different concentrations of phenylalanine (Phe) on the production of Taxol, antioxidant activity, and cytotoxic effects of extracts of suspension-cultured hazel cells were investigated. The cells were treated with different concentrations of Phe on day 7 of subculture and were harvested on day 14. The results showed that the amounts of Taxol and antioxidant activity were increased by increasing the phenylalanine supply. Interestingly, the cytotoxic effects of hazel cell extract were even stronger than that of pure Taxol (standard), suggesting hazel cell extract as a novel and suitable probe for treating human cancer. Application of phenylalanine to hazel cells exaggerates their effects.

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References

  1. Wani MC, Taylor HL, Wall ME, Coggon P, McPhail AT (1971) Plant antitumor agents. VI. The isolation and structure of Taxol, a novel antileukemic and antitumor agent from Taxus brevifolia. J Am Chem Soc 93:2325–2327

    Article  PubMed  CAS  Google Scholar 

  2. Kohler J, Goldspiel BR (1994) Evaluation of new drug Paclitaxel (Taxol). Pharmacotherapy 14:3–34

    PubMed  CAS  Google Scholar 

  3. Navia-Osorio A, Garden H, Cusido RM, Palazon J, Alfermann AW, Pinol MT (2002) Taxol and baccatin III production in suspension cultures of Taxus. J Plant Physiol 159:97–102

    Article  CAS  Google Scholar 

  4. Fett-neto AG, Melanson S, Sakata F, DiCosmo J (1993) Improved growth and Taxol yield in developing calli of Taxus cuspidata by medium composition modification. Biotechnology 11:1007–1012

    Article  Google Scholar 

  5. Tabata H (2004) Paclitaxel production by plant cell culture technology. Adv Biochem Eng Biotechnol 87:1–23

    PubMed  CAS  Google Scholar 

  6. Rezaei A, Ghanati F, Behmanesh M, Mokhtari-Dizaji M (2011) Ultrasound potentiated salicylic acid-induced physiological effects and production of Taxol in hazelnut (Corylus avellana L.) cell culture. Ultrasound Med Biol 37:1938–1947

    Article  PubMed  Google Scholar 

  7. Zhao J, Davis LC, Verpoorte R (2005) Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnol Adv 23:283–333

    Article  PubMed  CAS  Google Scholar 

  8. Wu J, Ge X (2004) Oxidative burst, jasmonic acid biosynthesis, and Taxol production induced by low-energy ultrasound in Taxus chinensis cell suspension cultures. Biotechnol Bioengeen 85: 714–721

    Google Scholar 

  9. Yu LJ, Lan WZ, QinWM XuH-B (2002) High stable production of taxol in elicited synchronous cultures of Taxus chinensis cells. Process Biochem 38:207–210

    Article  CAS  Google Scholar 

  10. Zhi M, Yuan YJ, Ren D (2004) Acting point in taxol biosynthesis pathway of elicitor in suspension cultures of Taxus chinensis var. mairei. Acta Bot Sin 46:730–737

    Google Scholar 

  11. Zhang CH, Fevereiro PS, He G, Chen Z (2007) Enhanced paclitaxel productivity and release capacity of Taxus chinensis cell suspension cultures adapted to chitosan. Plant Sci 172:158–163

    Article  CAS  Google Scholar 

  12. Ouyang J, Wang XD, Zhao B, Wang YC (2005) Enhanced production of phenylethanoid glycosides by precursor feeding to cell culture of Cistanche deserticola. Process Biochem 40:3480–3484

    Article  CAS  Google Scholar 

  13. Fett-neto AG, Melasan SJ, Nicholson SA (1994) Improved Taxol yield by aromatic carboxylic acid and amino acid feeding to cell culture of Taxus cuspidata. Biotechnol Bioeng 44:967–971

    Article  PubMed  CAS  Google Scholar 

  14. Hoffman A, Khan W, Worapong J, Strobel G, Griffin D, Arbogast B, Barofsky D, Boone RB, Ning L, Zheng P, Daley P (1998) Bioprospecting for Taxol in angiosperm plant extracts. Spectroscopy 13:22–32

    CAS  Google Scholar 

  15. Bestoso F, Ottaggio L, Balbi A, Damonte G, Degan P, Mazzei M, Cavalli F, Ledda B, Miele M (2006) In vitro cell cultures obtained from different explants of Corylus avellana produce Taxol and taxanes. BMC Biotechnol 6:45–48

    Article  PubMed  Google Scholar 

  16. Otaggio L, Bestoso F, Armirotti A, Balbi A, Damonte G, Mazzei M, Sancandi M, Miele M (2008) Taxanes from shells and leaves of Corylus avellana. J Nat Prod 7:58–60

    Article  Google Scholar 

  17. Jennewein S, Croteau R (2001) Taxol: biosynthesis, molecular genetics, and biotechnological applications. Appl Microbiol Biotechnol 57:13–19

    Article  PubMed  CAS  Google Scholar 

  18. Furmanowa M, Oledzka H, Syklowska-Baranek K, Jozefowicz J, Gieracka S (2000) Increased taxane accumulation in callus cultures of Taxus cuspidata and Taxus × media by some elicitors and precursors. Biotechnol Lett 22:1449–1452

    Article  CAS  Google Scholar 

  19. Rafat A, Koshy P, Sekaran M (2010) Antioxidant potential and content of phenolic compounds in ethanolic extracts of selected parts of Andrographis Paniculata. J Med Plants Res 4:197–202

    Article  CAS  Google Scholar 

  20. Gao H, Shupe TF, Hse CY, Eberhardt TL (2006) Antioxidant activity of extracts from the bark of Chamaecyparis lawsoniana (A. Murray) Parl. Holzforschung 60:459–462

    Article  CAS  Google Scholar 

  21. Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50:151–158

    Article  PubMed  CAS  Google Scholar 

  22. Veeresham C, Mamatha R (2003) Production of taxol and its analogues from cell cultures of Taxus wallichiana. Pharm Biol 4:426–430

    Article  Google Scholar 

  23. Wu J, Lin L (2003) Enhancement of taxol production and release in Taxus chinensis cell cultures by ultrasound, methyl jasmonate and in situ solvent extraction. Appl Microbiol Biotechnol 62:151–155

    Article  PubMed  CAS  Google Scholar 

  24. Chua MT, Tung YT, Chang ST (2007) Antioxidant activities of ethanolic extracts from the twigs of Cinnamum osmophloeum. Bioresource Technol 99:1918–1925

    Article  Google Scholar 

  25. Maikai VA, Kobo PI, Maikai BVO (2010) Antioxidant properties of Ximenia americana. Afr J Biotechnol 9:7744–7746

    Google Scholar 

  26. Oyaizu M (1986) Studies on product browning reaction prepared from glucosamine. J Nutr 44:307–315

    CAS  Google Scholar 

  27. Cai XX, Luo E, Yuan Q (2010) Interaction between Schwann cells and osteoblasts in vitro. Int J Oral Sci 2:74–81

    Article  PubMed  Google Scholar 

  28. Edhahiro JI, Nakamura M, Seki M, Furusaki S (2005) Enhanced accumulation of anthocyanin in cultured strawberry cells by repetitive feeding of l-phenylalanine into the medium. J Biosci Bioeng 99:43–47

    Article  Google Scholar 

  29. Luo J, He GY (2004) Optimization of elicitors and precursors for paclitaxel production in cell suspension culture of Taxus chinensis in the presence of nutrient feeding. Process Biochem 39:1073–1079

    Article  CAS  Google Scholar 

  30. Kubota N, Yakushiji H, Nishiyama N, Mimura H, Shimamura K (2001) Phenolic contents and l-phenylalanine ammonia-lyase activity in peach fruit as affected by rootstocks. J Japan Soc Hort Sci 70:151–156

    Article  CAS  Google Scholar 

  31. Demirci B, Kosar M, Demirci F (2007) Antioxidant activities of the essential oil of Chaerophyllum libanoticum Boiss. et Kotschy. Food Chem 105:1512–1517

    Article  CAS  Google Scholar 

  32. Kalpna R, Mital K, Sumitra C (2010) Vegetable and fruit peels as a novel source of antioxidants. J Med Plants Res 5:63–71

    Google Scholar 

  33. Chang ST, Wu JH, Wang SY, Kang PL, Yang NS, Shyur LF (2001) Antioxidant activity of extracts from Acacia confusa bark and heartwood. J Agric Food Chem 49:3420–3424

    Article  PubMed  CAS  Google Scholar 

  34. Kosinska A, Karamac M (2006) Antioxidant capacity of roasted health-promoting products. Pol J Food Nutr Sci 15:193–198

    CAS  Google Scholar 

  35. Colangelo D, Guo H, Konnors K, Kubota T, Silvestro S, Hoffman R (1992) Correlation of drug response in human tumors histocultured in vitro with an image-analysis MTT end point and in vivo xeno-grafted in nude mice. Anticancer Res 12: 1373–1376

    Google Scholar 

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Authors and Affiliations

  1. Department of Plant Biology, Faculty of Biological Science, Tarbiat Modares University (TMU), P.O. Box 14115-154, Tehran, Iran

    Ebrahim Bemani, Faezeh Ghanati, Ayatollah Rezaei & Mitra Jamshidi

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  1. Ebrahim Bemani
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  2. Faezeh Ghanati
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  3. Ayatollah Rezaei
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  4. Mitra Jamshidi
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Correspondence to Faezeh Ghanati.

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Bemani, E., Ghanati, F., Rezaei, A. et al. Effect of phenylalanine on Taxol production and antioxidant activity of extracts of suspension-cultured hazel (Corylus avellana L.) cells. J Nat Med 67, 446–451 (2013). https://doi.org/10.1007/s11418-012-0696-1

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  • DOI: https://doi.org/10.1007/s11418-012-0696-1

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