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Protective effect of Chresta martii extract against indomethacin-induced gastric lesions in mice

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Abstract

Chresta martii (Asteraceae) is a plant found in the Xingó region (semi-arid area) in Northeastearn Brazil, and is recognized by the local population as a traditional herb used to treat gastric diseases. This is the first report of the chemical composition, acute toxicity, and gastroprotective effect in mice of the hydroalcoholic extract (HAE) from the aerial parts (leaves and flowers) of Chresta martii. Animals received HAE doses from 10 to 2000 mg/kg, i.p. or 50 to 3000 mg/kg, p.o.) and were observed over 48 h for toxicity signs and mortality; sub-chronic toxicity was evaluated through 14 days treatment with once-daily HAE doses (400 mg/kg, p.o.). The gastroprotective effect of HAE was demonstrated on the indomethacin-induced gastric ulcer model after the administration of extracts. Data comparison of ulcer index averages between saline and HAE (100 or 400 mg/kg, p.o.) groups showed significant (P < 0.01) inhibition (71.73 and 76.72 %, respectively) of indomethacin-induced gastric lesions. Histological analyses showed significant (P < 0.05) inhibition of leukocyte migration in HAE-treated groups. A fingerprint of the HAE obtained by HPLC/UV/MS analysis showed major peaks characteristic of sesquiterpene lactones. Compound 1 was isolated and elucidated as a new natural product. Its capacity to prevent leukocyte chemotaxis was demonstrated in vitro, corroborating the pharmacological effects observed for C. martii HAE.

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Acknowledgments

This work was supported by Conselho Nacional de Desenvolvimento Científico e tecnológico (CNPq), Fundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico (FUNCAP), Programa Rede Nordeste de Biotecnologia (RENORBIO) and the Swiss National Science Foundation (Grant no. 200010-100083 to K. Hostettmann).

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Authors and Affiliations

  1. Laboratory of Pharmacology of Sobral (LaFS), Federal University of Ceará Brazil, Avenida Comandante Maurocélio Rocha Pontes, 100, Sobral, CE, 62042-280, Brazil

    A. A. R. Silva, M. M. Bezerra, H. V. Chaves, K. M. A. Pereira & J. A. Aguiar

  2. Laboratory of Biochemistry of Sobral, Master of Biotechnology Degree Program, Federal University of Ceará, Av. Comandante Maurocélio Rocha Pontes, 100, Sobral, CE, 62042-280, Brazil

    V. P. T. Pinto

  3. School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, 30 Quai Ernest-Ansermet, 1211, Geneva, Switzerland

    C. Abbet, C. A. Simões-Pires & K. Hostettmann

  4. Laboratory of Pharmacology of Bioactive Products, Department of Physiology and Pharmacology, Federal University of Pernambuco, Av. Prof. Moraes Rego 1235, Recife, PE, 50670-901, Brazil

    E. S. Franco & M. B. S. Maia

  5. Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, UFRGS, Av. Ipiranga 2752, Porto Alegre, RS, 90610-000, Brazil

    A. T. Henriques

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  1. A. A. R. Silva
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  2. M. M. Bezerra
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  3. H. V. Chaves
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  4. K. M. A. Pereira
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  5. J. A. Aguiar
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  6. V. P. T. Pinto
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  7. C. Abbet
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  8. C. A. Simões-Pires
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  9. E. S. Franco
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  10. A. T. Henriques
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  11. K. Hostettmann
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  12. M. B. S. Maia
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Correspondence to A. A. R. Silva.

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Silva, A.A.R., Bezerra, M.M., Chaves, H.V. et al. Protective effect of Chresta martii extract against indomethacin-induced gastric lesions in mice. J Nat Med 67, 143–151 (2013). https://doi.org/10.1007/s11418-012-0663-x

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  • DOI: https://doi.org/10.1007/s11418-012-0663-x

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