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Genetic variation of Croton stellatopilosus Ohba based on non-coding DNA sequences of ITS, trnK and trnL-F regions

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Abstract

Croton stellatopilosus Ohba (Plau-noi), a well-known Thai medicinal plant, was investigated for its genetic variation by analyzing three DNA regions, one nuclear internal transcribed spacer (ITS) region and two chloroplast trnL-F intergenic spacer and trnK intron regions. The results of ITS sequencing from 30 leaf samples showed that there were two major genotypes of C. stellatopilosus which were designated as STEL Type A and B. In addition, various nucleotide additive sequences which had presumably arisen from these two groups were also found. These so-called “putative hybrids”, interestingly, displayed trnK intron sequences identical to the STEL Type B but different from the Type A. For the trnL-F region, all the 30 samples showed identical sequences. Thus, it was suggested that in the hybridization of C. stellatopilosus, the Type A genotype acts as paternal parent whereas the Type B genotype acts as maternal parent. In addition, all C. stellatopilosus samples were analyzed for their plaunotol content using TLC densitometry. We found that the Type A genotype, hybrid group and Type B genotype had plaunotol content in the ranges 0.209–0.492, 0.319–0.896 and 0.442–1.000% (w/w) dry weight, respectively. The results indicated that there is a correlation between the plaunotol contents and non-coding DNA sequences of ITS, trnK and trnL-F regions of C. stellatopilosus.

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References

  1. Ogiso A, Kitazawa E, Mikuriya I, Promdej C (1978) Isolation and structure of antipeptic ulcer diterpene from Thai medicinal plant. Chem Pharm Bull 26:3117–3123

    PubMed  CAS  Google Scholar 

  2. Vongchareonsathit A, De-Eknamkul W (1998) Rapid TLC-densitometric analysis of plaunotol from Croton sublyratus leaves. Planta Med 64:279–280

    Article  PubMed  CAS  Google Scholar 

  3. Rinthong P, Jindaprasert A, De-Eknamkul W (2009) Simple TLC-densitometric analysis of plaunotol for screening of high-plaunotol containing plants of Croton stellatopilosus Ohba. J Planar Chromatogr 2:55–58

    Article  Google Scholar 

  4. Klinbantoom R (2004) ISSR analysis of Croton sublyratus Kurz. in Thailand. Master’s thesis, Department of Biotechnology, Faculty of Science, Chulalongkorn University

  5. Baldwin BG, Sanderson MJ, Porter JM, Wojciechowski MF, Cambell CS, Donoghue MJ (1995) The ITS region of nuclear ribosomal DNA: a valuable source of evidence on angiosperm phylogeny. Ann Missouri Bot Gard 82:247–277

    Article  Google Scholar 

  6. Campbell CS, Wojciechowski MF, Baldwin BG, Alice LA, Donoghue MJ (1997) Persistent nuclear ribosomal DNA sequence polymorphism in the Amelanchier Agamic complex (Rosaceae). Mol Biol Evol 14(1):81–90

    PubMed  CAS  Google Scholar 

  7. Chayamarit K, Welzen P (2005) Euphorbiaceae genera A-F. Flora of Thailand. The Forest Herbarium, National Park Wildlife and Plant Conservation Department, Bangkok, vol 8, pp 220–221

  8. White TJ, Bruns T, Lee S, Taylor JW (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocols: a guide to methods and applications. Academic Press, New York, pp 315–322

    Google Scholar 

  9. Taberlet P, Gielly L, Pautou G, Bouvet J (1991) Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant Mol Biol 17:1105–1109

    Article  PubMed  CAS  Google Scholar 

  10. Johnson LA, Soltis DE (1994) matK DNA sequences and phylogenetic reconstruction in Saxifragaceae s.s. Syst Bot 19:143–156

    Article  Google Scholar 

  11. Sang T, Crawford DJ, Stuessy TF (1995) Documentation of reticulate evolution in peonies (Paeonia) using internal transcribed spacer sequences of nuclear ribosomal DNA: implications for biogeography and concerted evolution. Proc Natl Acad Sci USA 92:6813–6817

    Article  PubMed  CAS  Google Scholar 

  12. Zhang JL, Zhang CQ, Gao LM, Yang JB, Li HT (2007) Natural hybridization origin of Rhododendron agastum (Ericaceae) in Yunnan, China: inferred from morphological and molecular evidence. J Plant Res 120:457–463

    Article  PubMed  CAS  Google Scholar 

  13. Tsukaya H (2004) Gene flow between Impatiens redicans and I. javernsis (Balsaminaceae) in Gunung Pangrango, Central Java, Indonesia. Am J Bot 91:2119–2123

    Article  CAS  Google Scholar 

  14. Whittall J, Liston A, Gisler S, Meinke RJ (2000) Detecting nucleotide additively from direct sequences is a SNAP: an example from Sidalcea (Malvaceae). Plant Biol 2:1–7

    Article  Google Scholar 

  15. Kondo K, Shiba M, Yamaji H, Morota T, Zhengmin C, Huixia P, Shiyama Y (2007) Species identification of licorice using nrDNA and cpDNA genetic markers. Biol Pharm Bull 30(8):1497–1502

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

We are grateful to the Institute of Biotechnology and Genetic Engineering (IBGE), Chulalongkorn University, for providing some specimens. We thank Kongkanda Chayamarit, PhD, of the Thai Royal Herbarium for helping us to identify plant specimens. This work was supported by the Commission on Higher Education, Thailand, to P. Rinthong for her PhD program scholarship.

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Authors and Affiliations

  1. Pharmaceutical and Natural Product Research Unit, Faculty of Pharmacy, Mahasarakham University, 44150, Maha Sarakham, Thailand

    Prasob-orn Rinthong

  2. Department of Medicinal Resources, Institute of Natural Medicine, University of Toyama, Toyama, Japan

    Shu Zhu & Katsuko Komatsu

  3. Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, Thailand

    Suchart Chanama

  4. Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand

    Wanchai De-Eknamkul

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  1. Prasob-orn Rinthong
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  2. Shu Zhu
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  3. Katsuko Komatsu
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  4. Suchart Chanama
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  5. Wanchai De-Eknamkul
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Correspondence to Prasob-orn Rinthong.

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Rinthong, Po., Zhu, S., Komatsu, K. et al. Genetic variation of Croton stellatopilosus Ohba based on non-coding DNA sequences of ITS, trnK and trnL-F regions. J Nat Med 65, 641–645 (2011). https://doi.org/10.1007/s11418-011-0536-8

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