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Inhibitory effects of devil’s claw (secondary root of Harpagophytum procumbens) extract and harpagoside on cytokine production in mouse macrophages

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Abstract

Successive oral administration (50 mg/kg) of a 50% ethanolic extract (HP-ext) of devil’s claw, the secondary root of Harpagophytum procumbens, showed a significant anti-inflammatory effect in the rat adjuvant-induced chronic arthritis model. HP-ext dose-dependently suppressed the lipopolysaccharide (LPS)-induced production of inflammatory cytokines [interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α)] in mouse macrophage cells (RAW 264.7). Harpagoside, a major iridoid glycoside present in devil’s claw, was found to be one of the active agents in HP-ext and inhibited the production of IL-1β, IL-6, and TNF-α by RAW 264.7.

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Acknowledgments

This work was financially supported by the “Antiaging Center Project” for Private Universities from the Ministry of Education, Culture, Sports, Science and Technology, 2008–2012.

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Authors and Affiliations

  1. Faculty of Pharmacy, Kinki University, 3-4-1 Kowakae, Higashi-osaka, Osaka, 577-8502, Japan

    Kazunori Inaba, Kazuya Murata, Shunsuke Naruto & Hideaki Matsuda

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  1. Kazunori Inaba
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  2. Kazuya Murata
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  3. Shunsuke Naruto
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  4. Hideaki Matsuda
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Correspondence to Hideaki Matsuda.

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Inaba, K., Murata, K., Naruto, S. et al. Inhibitory effects of devil’s claw (secondary root of Harpagophytum procumbens) extract and harpagoside on cytokine production in mouse macrophages. J Nat Med 64, 219–222 (2010). https://doi.org/10.1007/s11418-010-0395-8

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  • DOI: https://doi.org/10.1007/s11418-010-0395-8

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