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Effect of Gastrodia elata on tumor necrosis factor-alpha-induced matrix metalloproteinase activity in endothelial cells

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Abstract

The aim of the present study was to investigate whether an ethanol extract of Gastrodia elata (EGE) rhizome, a traditional Korean herbal medical food, suppresses the endothelial extracellular matrix degradation induced by tumor necrosis factor (TNF)-α. Gelatin zymography results showed that pretreatment with EGE to human umbilical vein endothelial cells (HUVEC) decreased TNF-α-induced increase of matrix metalloproteinase (MMP)-2/-9 activities in the range of 1–50 μg/ml. Real-time qRT-PCR results also revealed that TNF-α-induced MMP-2/-9 mRNA expression levels were attenuated by pretreatment with EGE. These results provide new insights into the pathophysiological mechanisms for the anti-atherosclerotic properties of EGE in vascular diseases.

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Acknowledgments

This work was supported by a research grant from the Korea Science and Engineering Foundation (KOSEF) funded by the Korean Government (MEST) (no. R13-2008-028-01000-0).

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Authors and Affiliations

  1. Professional Graduate School of Oriental Medicine, Wonkwang University, Iksan, Chonbuk, 570-749, Republic of Korea

    Yun Jung Lee, Sun Mi Hwang, Dae Gill Kang & Ho Sub Lee

  2. Medical Resources Research Institute (MeRRI), Wonkwang University, Iksan, Chonbuk, 570-749, Republic of Korea

    Yun Jung Lee & Ho Sub Lee

  3. Korea Institute of Oriental Medicine, Jeonmin-dong, Yusung-gu, Daejeon, 305-811, Republic of Korea

    Jin Sook Kim

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  1. Yun Jung Lee
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Correspondence to Ho Sub Lee.

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Lee, Y.J., Hwang, S.M., Kang, D.G. et al. Effect of Gastrodia elata on tumor necrosis factor-alpha-induced matrix metalloproteinase activity in endothelial cells. J Nat Med 63, 463–467 (2009). https://doi.org/10.1007/s11418-009-0352-6

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  • DOI: https://doi.org/10.1007/s11418-009-0352-6

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