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Quantitative analysis of ginsenosides Rb1, Rg1, and Re in American ginseng berry and flower samples by ELISA using monoclonal antibodies

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Abstract

Quantitative analysis of ginsenosides Rb1, Rg1, and Re in American ginseng berry and flower samples, which were collected in various months throughout the year, was performed by enzyme-linked immunosorbent assay (ELISA) using monoclonal antibodies. The American ginseng flower had the highest content of ginsenosides Rb1, Rg1, and Re (GRb1, GRg1, and GRe). The content of GRb1, GRg1, and GRe decreased in going from young through to ripe berry.

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Acknowledgments

We are grateful to Dr. Thomas S. C. Li at the Pacific Agri-Food Research Centre (Summerland, Canada) for donation of American ginsengs samples. This work was supported by the Japan Society for the Promotion of Science (JSPS) in 2005. This work was also funded in part by a Grant in Aid from the JSPS Asian CORE Program, the Ministry of Education, Culture, Sports, Science and Technology of Japan.

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Authors and Affiliations

  1. Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Chulalongkorn University, 254 Phyathai Road, Patumwan, Bangkok, 10330, Thailand

    Boonchoo Sritularak

  2. Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo, Nagasaki, 859-3298, Japan

    Osamu Morinaga & Yukihiro Shoyama

  3. Tang Center for Herbal Medicine Research, The Pritzker School of Medicine, The University of Chicago, 5841 S. Meryland Avenue, MC 4028, Chicago, IL, 60637, USA

    Chun-Su Yuan

  4. Department of Medicinal Plants Breeding and Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan

    Hiroyuki Tanaka

Authors
  1. Boonchoo Sritularak
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  2. Osamu Morinaga
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  3. Chun-Su Yuan
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  4. Yukihiro Shoyama
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  5. Hiroyuki Tanaka
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Correspondence to Hiroyuki Tanaka.

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Sritularak, B., Morinaga, O., Yuan, CS. et al. Quantitative analysis of ginsenosides Rb1, Rg1, and Re in American ginseng berry and flower samples by ELISA using monoclonal antibodies. J Nat Med 63, 360–363 (2009). https://doi.org/10.1007/s11418-009-0332-x

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  • DOI: https://doi.org/10.1007/s11418-009-0332-x

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