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The similar effect of transplantation of marrow-derived mesenchymal stem cells with or without prior differentiation induction in experimental myocardial infarction

  • Published:
Journal of Biomedical Science

Abstract

Marrow-derived mesenchymal stem cells (MSCs) have been heralded as a source of great promise for the regeneration of the infarcted heart. There is no clear data indicating whether or not in vitro differentiation of MSCs into major myocardial cells can increase the beneficial effects of MSCs. The aim of this study is to address this issue. To induce MSCs to transdifferentiate into cardiomyocyte-like and endothelial-like cells, 5-azacytidine and vascular endothelial growth factor (VEGF) were used, respectively. Myocardial infarction in rabbits was generated by ligating the left anterior descending coronary artery. Animals were divided into three experimental groups: I, control group; II, undifferentiated mesenchymal stem cell transplantation group; III, differentiated mesenchymal stem cell transplantation group; which respectively received peri-infarct injections of culture media, autologous undifferentiated MSCs and autologous differentiated MSCs. General pathology, immunohistochemistry, electron microscopy and echocardiography were performed in order to search for myocardial regeneration and improvement of cardiac function. In Groups II and III, implanted cells transdifferentiate into myocardial cells within 28 days post injection in a similar manner, and well-developed ultra structures formed within transplanted cells. Improvements in left ventricular function and reductions in infarcted area were observed in both cell-transplanted groups to the same degree. Vascular density was similar in Groups II and III and significantly higher in these groups compared with the control group. There is no need for prior differentiation induction of marrow-derived MSCs before transplantation and peri-infarct implantation of MSCs can efficiently regenerate the infarcted myocardium and improve cardiac function.

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Acknowledgements

We are grateful to Dr. Farzad Asadi for his help with statistical analysis. We thank the staff of Rastegar Central Research Laboratory of the Faculty of Veterinary Medicine as well as the staff of the Immunohistochemistry Department of Imam Khomeini Hospital Complex for their help in this study.

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Authors and Affiliations

  1. Department of Clinical Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

    Seyed Mahdi Nassiri & Zohreh Khaki

  2. Department of Hematology, School of Medical Sciences, Tarbiat Modares University, P.O. Box. 14115-111, Tehran, Iran

    Masoud Soleimani

  3. Tehran Heart Center, Medical Sciences, University of Tehran, Tehran, Iran

    Seyed Hossein Ahmadi, Shahram Rabbani, Mohammad Sahebjam & Mahmood Sheikh Fathollahi

  4. Department of Pathology, Imam Khomeini Hospital Complex, School of Medicine, University of Tehran, Tehran, Iran

    Issa Jahanzad & Farid Azmoudeh Ardalan

  5. Stem Cell Technology Company, Tehran, Iran

    Mahmood Sheikh Fathollahi

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  1. Seyed Mahdi Nassiri
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  2. Zohreh Khaki
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Correspondence to Masoud Soleimani.

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Nassiri, S.M., Khaki, Z., Soleimani, M. et al. The similar effect of transplantation of marrow-derived mesenchymal stem cells with or without prior differentiation induction in experimental myocardial infarction. J Biomed Sci 14, 745–755 (2007). https://doi.org/10.1007/s11373-007-9188-9

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  • DOI: https://doi.org/10.1007/s11373-007-9188-9

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