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Toxicogenomics of A375 human malignant melanoma cells treated with arbutin

  • Published:
Journal of Biomedical Science

Summary

Although arbutin is a natural product and widely used as an ingredient in skin care products, its effect on the gene expression level of human skin with malignant melanoma cells is rarely reported. We aim to investigate the genotoxic effect of arbutin on the differential gene expression profiling in A375 human malignant melanoma cells through its effect on tumorigenesis and related side-effect. The DNA microarray analysis provided the differential gene expression pattern of arbutin-treated A375 cells with the significant changes of 324 differentially expressed genes, containing 88 up-regulated genes and 236 down-regulated genes. The gene ontology of differentially expressed genes was classified as belonging to cellular component, molecular function and biological process. In addition, four down-regulated genes of AKT1, CLECSF7, FGFR3, and LRP6 served as candidate genes and correlated to suppress the biological processes in the cell cycle of cancer progression and in the downstream signaling pathways of malignancy of melanocytic tumorigenesis.

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Authors and Affiliations

  1. Institute of Medicine, Chung Shan Medical University, Taichung, 40242, Taiwan

    Sun-Long Cheng & Jin-Nan Sheu

  2. School of Nutrition, Chung Shan Medical University, Taichung, 40242, Taiwan

    Rosa Huang Liu

  3. Institute of Biological Chemistry and Genomics Research Center, Academia Sinica, Taipei, 11529, Taiwan

    Shui-Tein Chen & Supachok Sinchaikul

  4. Institute of Biochemical Sciences, College of Life Science, National Taiwan University, Taipei, 10617, Taiwan

    Shui-Tein Chen

  5. Institute of Immunology, Chung Shan Medical University, 110 Sec. 1, Chien-Kuo N. Road, Taichung, 40242, Taiwan

    Gregory Jiazer Tsay

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  1. Sun-Long Cheng
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  2. Rosa Huang Liu
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  3. Jin-Nan Sheu
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Correspondence to Gregory Jiazer Tsay.

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Cheng, SL., Liu, R.H., Sheu, JN. et al. Toxicogenomics of A375 human malignant melanoma cells treated with arbutin. J Biomed Sci 14, 87–105 (2007). https://doi.org/10.1007/s11373-006-9130-6

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  • DOI: https://doi.org/10.1007/s11373-006-9130-6

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