Summary
Hepatocellular carcinoma (HCC) is a leading cause of death worldwide. Hepatitis B virus (HBV) or hepatitis C virus (HCV) infection has been shown to cause hepatic carcinogenesis. A total 58,251 of cDNA clones of full-length cDNA libraries of HBV and HCV-infected HCC and their surrounding non-tumor tissues, respectively, were sequenced and analyzed by blasting against GENEBANK maintained by NCBI. About 180 and 279 of genes were shown an obviously increased and decreased expression patterns between HCC tissue and its adjacent non-tumor tissue. The candidate genes consisted of the genes encoded liver specific metabolism enzymes, secretory functional proteins, proteases and their inhibitors, protein chaperon, cell cycle components, apoptosis-related proteins, transcriptional factors, and DNA binding proteins. Several genes were further investigated by using real-time PCR to confirm the gene expression levels in at least 24 pairs of HCC tissues and adjacent non-tumor tissues. The results showed that genes encoded reticulon 4, RGS-1, antiplasmin, and kallikrein B were down-regulated with the average of 2.8, 8.5, 3.2, and 10.5-fold, respectively. Our results provide crucial candidate genes to develop clinical diagnosis and gene therapy of HCC.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Buendia M.A. (2000). Genetics of hepatocellular carcinoma. Semin. Cancer Biol. 10:185–200
Feitelson M.A, Sun B., Satiroglu Tufan N.L., Liu J., Pan J., Lian Z. (2002). Genetic mechanisms of hepatocarcinogenesis. Oncogene 21:2593–2604
Thorgeirsson S.S., Grisham J.W. (2002). Molecular pathogenesis of human hepatocellular carcinoma. Nat. Genet. 31:339–346
Bosch F.X., Ribes J., Borras J. (1999). Epidemiology of primary liver cancer. Semin Liver Dis. 19:271–285
Fattovich G., Stroffolini T., Zagni I., Donato F. (2004). Hepatocellular carcinoma in cirrhosis: incidence and risk factors. Gastroenterology 127:S35-S50
Sell S. (2001). Heterogeneity and plasticity of hepatocyte lineage cells. Hepatology 33:738–750
Tsutsumi T., Suzuki T., Moriya K., Yotsuyanagi H., Shintani Y., Fujie H., Matsuura Y., Kimura S., Koike K., Miyamura T. (2002). Alteration of intrahepatic cytokine expression and AP-1 activation in transgenic mice expressing hepatitis C virus core protein. Virology 304:415–424
Chen X., Cheung S.T., So S., Fan S.T., Barry C., Higgins J., Lai K.M., Ji J., Dudoit S., Ng I.O., Van De R.M., Botstein D., Brown P.O. (2002). Gene expression patterns in human liver cancers. Mol. Biol. Cell. 13:1929–1939
Zondervan P.E., Wink J., Alers J.C., IJzermans J.N., Schalm S.W., de Man R.A., van Dekken H. (2000). Molecular cytogenetic evaluation of virus-associated and non-viral hepatocellular carcinoma: analysis of 26 carcinomas and 12 concurrent dysplasias. J. Pathol. 192(2):207–15
Niketeghad F., Decker H.J., Caselmann W.H., Lund P., Geissler F., Dienes H.P., Schirmacher P. (2001). Frequent genomic imbalances suggest commonly altered tumour genes in human hepatocarcinogenesis. Br. J. Cancer 85:697–704
Raidl M., Pirker C., Schulte-Hermann R., Aubele M., Kandioler-Eckersberger D., Wrba F., Micksche M., Berger W., Grasl-Kraupp B. (2004). Multiple chromosomal abnormalities in human liver (pre)neoplasia. J. Hepatol. 40:60–8
Levine A.J., Momand J., Finlay C.A. (1991). The p53 tumour suppressor gene. Nature 351:453–456
Thomas M., Kalita A., Labrecque S., Pim D., Banks L., Matlashewski G. (1999). Two polymorphic variants of wild-type p53 differ biochemically and biologically. Mol. Cell. Biol. 19:1092–1100
Storey A., Thomas M., Kalita A., Harwood C., Gardiol D., Mantovani F., Breuer J., Leigh I.M., Matlashewski G., Banks L. (1998). Role of a p53 polymorphism in the development of human papillomavirus-associated cancer. Nature 393:229–234
Suzuki Y., Sugano S. (2001). Construction of full-length-enriched cDNA libraries. The oligo-capping method. Methods Mol. Biol. 175:143–153
Suzuki Y., Yoshitomo-Nakagawa K., Maruyama K., Suyama A. Sugano S. (1997). Construction and characterization of a full length-enriched and a 5′-end-enriched cDNA library. Gene 200:149–156
Watari A., Yutsudo M. (2003). Multi-functional gene ASY/Nogo/RTN-X/RTN4: apoptosis, tumor suppression, and inhibition of neuronal regeneration. Apoptosis. 8:5–9
Goretzki L., Schmitt M., Mann K., Calvete J., Chucholowski N., Kramer M., Gunzler W. A., Janicke F., Graeff H. (1992). Effective activation of the proenzyme form of the urokinase-type plasminogen activator (pro-uPA) by the cysteine protease cathepsin L. FEBS Lett. 297:112–118
Gho Y.S., Yoon W.H., Chae C.B. (2002). Antiplasmin activity of a peptide that binds to the receptor-binding site of angiogenin. J. Biol. Chem. 277:9690–9694
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Tsai, CC., Huang, KW., Chen, HF. et al. Gene expression analysis of human hepatocellular carcinoma by using full-length cDNA library. J Biomed Sci 13, 241–249 (2006). https://doi.org/10.1007/s11373-005-9062-6
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11373-005-9062-6