Summary
Chronic infections with hepatitis B and C viruses (HBV and HCV) are etiologically linked to hepatitis, liver cirrhosis, and hepatocellular carcinoma (HCC). Both viruses may induce activation of nuclear factor-kappa B (NF-κB) in hepatocytes that plays a crucial role in the regulation of cell growth and apoptosis. Functional proteomics analysis of proteins associated with NF-κB signaling complexes in both viruses-related HCC tumor and non-tumor tissues may disclose possible common mechanisms in hepatocarcinogenesis. By functional proteomics, we analyzed proteins associated with NF-κB-signaling complexes in four-paired human HCC tumor and non-tumor tissues from HBV- and HCV-infected patients, respectively, and in one-paired tissue with dual viral infection. The quantity of NF-κB-associated proteins was semi-quantitatively measured by protein spot intensity on the gels of two-dimensional polyacrylamide gel electrophoresis. The results showed that overexpression of NF-κB-associated Wnt-1 protein in tumor part was detected in the␣majority of HBV- and HCV-infected HCC samples. These data suggest that enhanced expression of NF-κB-associated Wnt-1 protein might be a mechanism of hepatocarcinogenesis common to HBV- and HCV-infected patients. NF-κB signaling pathway and Wnt-1 protein could be potential targets for designing highly effective therapeutic agents in treating HCC and for chemoprevention of hepatocarcinogenesis.
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Abbreviations
- m/z:
-
mass-to-charge ratio
- 2-DE:
-
two-dimensional polyacrylamide gel electrophoresis
- EMSA:
-
electrophoretic mobility shift assay
- HBV:
-
hepatitis B virus
- HCC:
-
hepatocellular carcinoma
- HCV:
-
hepatitis C virus
- HSP:
-
heat shock protein
- MALDI-Q-TOF:
-
matrix-assisted laser desorption/ionization-quadrupole-time-of-flight
- MS:
-
mass spectrometry
- NF-κB:
-
nuclear factor-kappa B
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Acknowledgement
This work was supported in part by grants from the National Science Council, Taipei, Taiwan [NSC 91-2314-B-384-007 MH]; and the Chi-Mei Foundation CMFHT9102, Tainan, Taiwan, to S.L. Tsai.
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Lee, TH., Tai, DI., Cheng, CJ. et al. Enhanced nuclear factor-kappa B-associated Wnt-1 expression in hepatitis B- and C-related hepatocarcinogenesis: identification by functional proteomics. J Biomed Sci 13, 27–39 (2006). https://doi.org/10.1007/s11373-005-9030-1
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DOI: https://doi.org/10.1007/s11373-005-9030-1