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Overexpression of an Aurora-C kinase-deficient mutant disrupts the Aurora-B/INCENP complex and induces polyploidy

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Journal of Biomedical Science

Abstract

Aurora kinases are emerging as key regulators of centrosome function, chromosome segregation and cytokinesis. We previously isolated Aurora-C (Aie1), a third type of Aurora kinase, in a screen for kinases expressed in mouse sperm and eggs. Currently, we know very little about the precise localization and function of Aurora-C. Immunofluorescence analysis of ectopically expressed GFP-Aurora-C has revealed that Aurora-C is a new member of the chromosomal passenger proteins localizing first to the centromeres and then to the central spindles during cytokinesis. In order to study the potential role of Aurora-C, we examined the effects of a kinase-deficient (KD) mutant (AurC-KD) in HeLa Tet-Off cells under tetracycline control. Our results showed that overexpression of AurC-KD causes defects in cell division and induces polyploidy and apoptosis. Interestingly, AurC-KD overexpression also inhibits centromere/kinetochore localization of Aurora-B, Bub1, and BubR1, reduces histone H3 phosphorylation, and disrupts the association of INCENP with Aurora-B. Together, our results showed that Aurora-C is a chromosomal passenger protein, which may serve as a key regulator in cell division.

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  1. Institute of Biomedical Sciences, Academia Sinica, 128, Academia Road, Sec. 2, Taipei, 11529, Taiwan, ROC

    Hua-Ling Chen, Chieh-Ju C. Tang, Chiung-Ya Chen & Tang K. Tang

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  1. Hua-Ling Chen
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  2. Chieh-Ju C. Tang
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  3. Chiung-Ya Chen
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  4. Tang K. Tang
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Correspondence to Tang K. Tang.

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Chen, HL., Tang, CJ.C., Chen, CY. et al. Overexpression of an Aurora-C kinase-deficient mutant disrupts the Aurora-B/INCENP complex and induces polyploidy. J Biomed Sci 12, 297–310 (2005). https://doi.org/10.1007/s11373-005-0980-0

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  • DOI: https://doi.org/10.1007/s11373-005-0980-0

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