Skip to main content

Advertisement

SpringerLink
Log in

Effect of humic preparation on winter wheat productivity and rhizosphere microbial community under herbicide-induced stress

  • Humic Substances and Nature-like Technologies
  • Published:
Journal of Soils and Sediments Aims and scope Submit manuscript

Abstract

Purpose

The aim of the present work was to study the effect of humic preparation on the yielding capacity of winter wheat, the dynamics of mineral nutrients in the rhizosphere, and the activity of rhizosphere microbial community, as well as the protective properties of humate treatment under the stress caused by the application of a sulfonylurea herbicide.

Materials and methods

The humic preparation BIO-Don is prepared from vermicompost by alkaline extraction. The effect of humic preparation was estimated in a field experiment. The contents of the mobile ammonium and nitrate, exchangeable potassium, phosphorus, and humus were determined. Sampling and nutrient determination were performed in the tillering, booting, and grain maturing stages. Production experiments on the study of the combined effect of the humic preparation and the sunfonylurea pesticide Granstar Pro were performed in 2015 and 2017. The dynamics of mobile phosphorus in soil was studied. The abundance of culturable bacteria in the rhizosphere and bacterial species with different ecological strategies was determined. The dominant bacterial species were identified by MALDI-TOF mass spectrometry.

Results and discussion

The results of a small-plot experiment showed that at the tillering stage, the use of humic preparation has led to significantly higher supply of soil with mobile phosphorus. The winter wheat yield was 32.0 dt/ha in the control and 39.2 dt/ha with humic preparation or 22.5% gain in yield. The results of production experiment show that the application of sulfonylurea herbicide induced a chemical stress on winter wheat plants, but the use of humic preparation reduced this effect and increased the availability of phosphorus compounds. The treatment of plants with pesticides caused the general decrease in abundance of bacteria in the rhizosphere. The effect on quickly growing bacteria is more pronounced, while slowly growing bacteria and fungi are more resistant to this impact.

Conclusions

The application of humic preparation to winter wheat plantations allows to decrease the toxic effect of sulfonylurea herbicide, improve the supply of soil with mineral nutrients, and increase the crop yield. The favorable effects of humic preparation can be related to the active regulation of phosphorus mobilization by plants through the mechanism of root exudates due to the activation of root microbiota.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Akladious SA, Mohamed HI (2018) Ameliorative effects of calcium nitrate and humic acid on the growth, yield component and biochemical attribute of pepper (Capsicum annuum) plants grown under salt stress. Sci Hortic 236:244–250

    Article  CAS  Google Scholar 

  • Al-Fraihat AH, Al-Tabbal JA, Abu-Darwish MS, Alhrout HH, Hasan HS (2018) Response of onion (Allium cepa) crop to foliar application of humic acid under rain-fed conditions. Int J Agric Biol 20(5):1235–1241

    CAS  Google Scholar 

  • Al-Maliki S, AL-Mammory H, Scullion J (2018) Interactions between humic substances and organic amendments affecting soil biological properties and growth of Zea mays L. in the arid land region. Arid Land Res Man 1-16. https://doi.org/10.1080/15324982.2018.1495670

  • Bezuglova OS, Polienko EA (2011) Application of humic preparations for potato and winter wheat. Problemy Agrokhimii i Ekologii 4:29–32 (in Russian)

    Google Scholar 

  • Bezuglova OS, Tver’yanovich IS (2004) Effect of iron humates on the floral productivity of rose “red velvet” and its resistance to phytopathogens on calcareous chernozems. Izv Vyssh Uchebn Zav Sev-Kavkaz Reg Estestv Nauki 4:96–99 (in Russian)

    Google Scholar 

  • Brown AL, Jackson WR, Cavagnaro TR (2014) A meta-analysis and review of plant-growth response to humic substances: practical implications for agriculture. Adv Agron 124:37

    Article  Google Scholar 

  • Bryutova NM, Bezuglova OS (2016) Humic preparations as growth regulators for ornamental (woody) plants. Subtrop Dekor Sadov 57:88–96 (in Russian)

    Google Scholar 

  • Canellas LP, Olivares FL (2014) Physiological responses to humic substances as plant growth promoter. Chem Biol Technol Agric 1(1):3

    Article  Google Scholar 

  • Canellas LP, Olivares FL, Aguiar NO, Jones DL, Nebbioso A, Mazzei P, Piccolo A (2015) Humic and fulvic acids as biostimulants in horticulture. Sci Hortic 196:15–27

    Article  CAS  Google Scholar 

  • Ciarkowska K, Sołek-Podwika K, Filipek-Mazur B, Tabak M (2017) Comparative effects of lignite-derived humic acids and FYM on soil properties and vegetable yield. Geoderma 303:85–92

    Article  CAS  Google Scholar 

  • Ćwieląg-Piasecka I, Medyńska-Juraszek A, Jerzykiewicz M, Dębicka M, Bekier J, Jamroz E, Kawałko D (2018) Humic acid and biochar as specific sorbents of pesticides. J Soils Sediments 18(8):2692–2702

    Article  Google Scholar 

  • Danyaei A, Has-sanpour S, Baghaee MA, Dabbagh M, Babarabie M (2017) The effect of sulfur containing humic acid on yield and nutrient uptake in olive fruit. Open J Ecol 7:279–288

    Article  Google Scholar 

  • De Leij FAAM, Whipps JM, Lynch JM (1994) The use of colony development for the characterization of bacterial communities in soil and on roots. Microb Ecol 27(1):81–97

    Article  Google Scholar 

  • Đorđević S, Najdenovska O, Cvijanović G (2010) The influence of Casaron [ie Casoron] and Starane herbicides on micro flora in soil grown with vineyard. Soil and Plant 59(1):31–47

  • Dospekhov BA (1985) Methods of field experiments Agropromizdat, Moscow (in Russian)

  • El-Mohamedy RSR, Shafeek MR, Abd El-Samad EEDH, Salama DM, Rizk FA (2017) Field application of plant resistance inducers (PRIs) to control important root rot diseases and improvement growth and yield of green bean (Phaseolus vulgaris L.). Austr J Crop Sci 11(5):496–505

    Article  CAS  Google Scholar 

  • Ghanbarpour E, Rezaei M, Lawson S (2018) Reduction of cracking in pomegranate fruit after foliar application of humic acid, calcium-boron and kaolin during water stress. Erwerbs-obstbau 1–9. https://doi.org/10.1007/s10341-018-0386-6

  • Gomes NCM, Heuer H, Schönfeld J, Costa R, Mendonca-Hagler L, Smalla K (2001) Bacterial diversity of the rhizosphere of maize (Zea mays) grown in tropical soil studied by temperature gradient gel electrophoresis. Plant Soil 232(1–2):167–180

    Article  CAS  Google Scholar 

  • Hosseini Farahi M, Aboutalebi A, Jowkar MM (2017) Effect of different media substrate and humic acid on growth and nutrient absorption of soilless cultured cut rose flowers. J Sci Technol Greenhouse Cult Isfahan Univ Technol 8(2):89–103

    Article  Google Scholar 

  • Ivanov VP (1973) Plant exudates and their significance in the life of phytocenoses. Nauka, Moscow (in Russian)

    Google Scholar 

  • Kadhim RA, Hussein ASJ, Jumaa FF (2017) Effect of organic fertilizer extract (x–humate 85) and application method on growth and yield of apricot trees Labeeb-1 cv. Iraqi J Agric Sci 48(4):1108

    Google Scholar 

  • Khan A, Ahmed N, Shah SA (2018) Effect of humic acid on fruit yield attributes, yield and leaf nutrient accumulation of apple trees under calcareous soil. Indian J Sci Technol 11(15):1–8

    Article  Google Scholar 

  • Khomyakov YuV (2009) Role of root exudates in the formation of biochemical properties of the rhizosphere zone. Dissertation, St Petersburg, p 144 (in Russian)

  • Kotelev VV (1964) Role microorganisms in the decomposition of organic phosphates and the migration of phosphorus in the soil. Dissertation, Institute of Microbiology, USSR Academy of Sciences (in Russian)

  • Kozdrój J, van Elsas JD (2000) Response of the bacterial community to root exudates in soil polluted with heavy metals assessed by molecular and cultural approaches. Soil Biol Biochem 32(10):1405–1417

    Article  Google Scholar 

  • Lay JO Jr (2001) MALDI-TOF mass spectrometry of bacteria. Mass Spectrom Rev 20(4):172–194

    Article  CAS  Google Scholar 

  • Lipczynska-Kochany E (2018) Humic substances, their microbial interactions and effects on biological transformations of organic pollutants in water and soil: a review. Chemosphere 202:420–437

    Article  CAS  Google Scholar 

  • Lizarazo LM, Jordá JD, Juárez M, Sánchez-Andreu J (2005) Effect of humic amendments on inorganic N, dehydrogenase and alkaline phosphatase activities of a Mediterranean soil. Biol Fertil Soils 42(2):172–177

    Article  CAS  Google Scholar 

  • Maji D, Misra P, Singh S, Kalra A (2017) Humic acid rich vermicompost promotes plant growth by improving microbial community structure of soil as well as root nodulation and mycorrhizal colonization in the roots of Pisum sativum. Appl Soil Ecol 110:97–108

    Article  Google Scholar 

  • Margesin R, Płaza GA, Kasenbacher S (2011) Characterization of bacterial communities at heavy-metal-contaminated sites. Chemosphere 82(11):1583–1588

    Article  CAS  Google Scholar 

  • Melo ROD, Oliveira HPD, Silveira KC, Baldotto LEB, Baldotto MA (2018) Initial performance of maize in response to humic acids and plant growth-promoting bacteria. Revista Ceres 65(3):271–277

    Article  Google Scholar 

  • Nowick W (2014) Actual results of improvement of biological soil fertility indicators after application of phytohumic combination (PHCs) in the program tandem12/21 (2012-2021) Proceedings of the Tenth International Conference daRostim 2014 «Humic Substances and Other Biologically Active Compounds in Agriculture», Moscow, pp 255–264 (in Russian)

  • Olaetxea M (2015) Effects of a sedimentary humic acid on plant growth: study of the general mechanisms of action upon root application in cucumber plants (Cucumis sativus L. cv Ashley). Doctoral Dissertation. Universidad de Navarra, Pamplona, p 174

  • Olaetxea M, De Hita D, Garcia CA, Fuentes M, Baigorri R, Mora V, Garnica M, Urrutia O, Erro J, Zamarreño AM, Berbara RL, Garcia-Mina JM (2018) Hypothetical framework integrating the main mechanisms involved in the promoting action of rhizospheric humic substances on plant root- and shoot- growth. Appl Soil Ecol 123:521–537

  • Olivares FL, Busato JG, Paula AM, Lima LS, Aguiar NO, Canellas LP (2017) Plant growth promoting bacteria and humic substances: crop promotion and mechanisms of action. Chem Biol Technol Agric 4(1):30

    Article  Google Scholar 

  • Pansuriya PB, Varu DK, Viradia RR (2018) Response of biostimulants and biofertilizers on corm, cormels and spike yield of gladiolus (Gladiolus grandiflorus L) cv. American beauty under greenhouse conditions. Int J Chem Stud 6(2):2659–2663

    Google Scholar 

  • Polienko EA, Bezuglova OS, Gorovtsov AV, Lykhman VA, Shimko AE, Bondareva AM, Zakharova IA (2015) Effect of humic fertilizer BIO-Don on the grain quality of soft winter wheat DonEco. Izv Oregburg Gos Univ 53:171–173 (in Russian)

    Google Scholar 

  • Rasheed SM, Abdullah HM, Ali ST (2017) Response of two hybrids of tomato (Lycupersicun esculentum Mill.) to four concentration of humic acid fertilizers in plastic house conditions. J Tikrit Univ Agri Sci 17(1):1–12

    Google Scholar 

  • Sarwar M, Ahmed S, Arsalan M, Khan M (2017) Humic acid affects zinc availability and wheat yield in zinc deficient calcareous soil. J Appl Agric Biotechnol 2(1):19–25

    Google Scholar 

  • Seng P, Drancourt M, Gouriet F, La Scola B, Fournier PE, Rolain JM, Raoult D (2009) Ongoing revolution in bacteriology: routine identification of bacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Clin Infect Dis 49(4):543–551

    Article  CAS  Google Scholar 

  • Shokri S, Tadayon MR (2018) Effect of different nutrient solutions on yield and potato minituber production under aeroponic and hydroponic cultures. J Sci Technol Greenhouse Cult –Isfahan Univ Technol 8(4):79–89

    Article  Google Scholar 

  • Sigler WV, Zeyer J (2004) Colony-forming analysis of bacterial community succession in deglaciated soils indicates pioneer stress-tolerant opportunists. Microb Ecol 48(3):316–323

    Article  CAS  Google Scholar 

  • Taspinar MS, Aydin M, Sigmaz B, Yildirim N, Agar G (2017) Protective role of humic acids against Picloram-induced genomic instability and DNA methylation in Phaseolus vulgaris. Environ Sci Pollut Res 24(29):22948–22953

    Article  CAS  Google Scholar 

  • Ud Din I, Sarwar M, Khan QU, Khan MJ, ud Din F, ud Din F (2018) 22Onion (Allium cepa L.) yield and phosphorus use efficiency as affected by plant extracted humic acid application. Pure Appl Biol 7(2):590–594

    CAS  Google Scholar 

  • Vasić V, Jarak M, Đurić S, Orlović S, Pekeč S (2010) Microbial activity of rhizospheric soil in the poplar nurseries and plantations. Poplar 185:51–60

    Google Scholar 

  • Vasic V, Djuric S, Jafari-Hajnal T, Orlovic S, Vasic S, Pajnik LP, Galović V (2018) The microbiological response of forest soils after application of nicosulfuron, imazamox and cycloxydim. Int J Environ Sci Technol. https://doi.org/10.1007/s13762-018-1862-0

  • Wu Y, Li Y, Ma YD, Zhang L, Ren ZM, Xia YP (2017) Hormone and antioxidant responses of Lilium Oriental hybrids ‘Sorbonne’ bulblets to humic acid treatments in vitro. J Hortic Sci Biotechnol 92(2):155–167

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Southern Federal University, Rostov-on-Don, Russia

    Olga S. Bezuglova, Andrey V. Gorovtsov & Aleksander Demidov

  2. Federal Rostov Agricultural Research Center, Rassvet, Rostov Region, Russia

    Olga S. Bezuglova, Andrey V. Gorovtsov, Elena A. Polienko, Vladimir E. Zinchenko, Artyom V. Grinko, Vladimir A. Lykhman & Marina N. Dubinina

Authors
  1. Olga S. Bezuglova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Andrey V. Gorovtsov
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Elena A. Polienko
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Vladimir E. Zinchenko
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Artyom V. Grinko
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Vladimir A. Lykhman
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Marina N. Dubinina
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Aleksander Demidov
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Andrey V. Gorovtsov.

Additional information

Responsible editor: José María García-Mina

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

ESM 1

(DOCX 25 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Bezuglova, O.S., Gorovtsov, A.V., Polienko, E.A. et al. Effect of humic preparation on winter wheat productivity and rhizosphere microbial community under herbicide-induced stress. J Soils Sediments 19, 2665–2675 (2019). https://doi.org/10.1007/s11368-018-02240-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11368-018-02240-z

Keywords

Search

Navigation