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Development of microbial community structure in vegetable-growing soils from open-field to plastic-greenhouse cultivation based on the PLFA analysis

  • Soils, Sec 2 • Global Change, Environ Risk Assess, Sustainable Land Use • Research Article
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Abstract

Purpose

The objectives of this study were to investigate (i) how the changes in cultivation pattern of vegetable affect soil microbial communities and (ii) the relationships between soil physico-chemical properties and microbial community structure.

Materials and methods

Soil samples were collected from fields growing vegetable crops with various times of plastic-greenhouse cultivation (0, 1, 4, 7 and 15 years, respectively). Phospholipid fatty acid (PLFA) analysis was conducted to reveal the soil microbial community of the test soils.

Results and discussion

The open-field soil had the highest total PLFAs amount. Although the Shannon-Weaver index was also highest in the open-field soil, the difference was not significant. Plastic-greenhouse cultivation decreased PLFAs species diversity and richness. Cluster analysis and principal component analysis (PCA) of the PLFA profiles revealed distinct groupings at different times during plastic-greenhouse cultivation.

Conclusions

Ultimately, PLFA analyses showed that long-term plastic-greenhouse cultivation make the physiological status of soil microbial community worse and increased stress level of microorganisms. And soil microbial community was significantly affected by field water capacity and water-soluble organic carbon. The study highlights the potential risk of long-term plastic-greenhouse cultivation to soil microbial community.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (no. 40971255), and the Fundamental Research Funds for the Central Universities.

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Authors and Affiliations

  1. Institute of Soil and Water Resources and Environmental Science, College of Environmental and Resource Sciences, Zhejiang Provincial Key Laboratory of Agricultural Resources and Environment, Zhejiang University, Hangzhou, 310058, China

    Zhiyuan Yao, Jiajia Xing, Haiping Gu, Haizhen Wang, Jianjun Wu, Jianming Xu & Philip C. Brookes

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  1. Zhiyuan Yao
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  2. Jiajia Xing
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  3. Haiping Gu
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  4. Haizhen Wang
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  5. Jianjun Wu
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  7. Philip C. Brookes
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Correspondence to Haizhen Wang.

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Responsible editor: Chengrong Chen

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Yao, Z., Xing, J., Gu, H. et al. Development of microbial community structure in vegetable-growing soils from open-field to plastic-greenhouse cultivation based on the PLFA analysis. J Soils Sediments 16, 2041–2049 (2016). https://doi.org/10.1007/s11368-016-1397-2

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  • DOI: https://doi.org/10.1007/s11368-016-1397-2

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