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Can root exudate components influence the availability of pyrene in soil?

  • SOILS, SEC 3 • REMEDIATION AND MANAGEMENT OF CONTAMINATED OR DEGRADED LANDS • RESEARCH ARTICLE
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Journal of Soils and Sediments Aims and scope Submit manuscript

Abstract

Purpose

Little information is currently available regarding the influence of different root exudate components (RECs) on the availability of persistent organic pollutants in the soil environment. In this study, we investigated the impacts of different RECs including organic acids, amino acids, and fructose on the availability of pyrene as a representative polycyclic aromatic hydrocarbon (PAH) in soils.

Materials and methods

Citric acid, oxalic acid, malic acid, serine, alanine, and fructose were used in the experiments as representative RECs. Pyrene-spiked soils (TypicPaleudalfs) with present RECs were incubated for 30 days, and the available fraction of pyrene was determined using n-butanol extraction procedure.

Results and discussion

The amount of n-butanol-extractable pyrene in soil increased with the addition of tested RECs and increased when REC concentrations are enhanced within the range of 0–21 g kg−1. The extractability of pyrene in soil with REC treatments and the enhancement ratio (r, %) of the extractable pyrene in soil by the addition of RECs after a 30-day incubation decreased in the following order: organic acids (oxalic acid ≥ citric acid > malic acid) > amino acid (alanine > serine) > fructose. This decrease was observed irrespective of soil sterilization, although the concentrations of extractable pyrene were lower in non-sterilized soils compared to sterilized soils. The concentrations of metal cations and dissolved organic matter (DOM) in solution increased when organic acids were added.

Conclusions

The tested RECs at concentrations of 0–21 g kg−1 clearly enhanced the availability of pyrene in soils, and larger amounts of RECs resulted in higher pyrene availabilities in the tested soils. Microbial biodegradation diminished the amount of available pyrene irrespective of the presence of RECs. The mechanism of REC-influenced availability of pyrene in soil may be related to the metal dissolution and release of DOM from soil solids. The results of this study will be useful in assessing PAH-related risks to human health and the environment and will be instructive in food safety and remediation strategies at contaminated sites.

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References

  • Alkorta I, Garbisu C (2001) Phytoremediation of organic contaminants in soils. Bioresour Technol 79:273–276

    Article  CAS  Google Scholar 

  • An CJ, Huang GH, Wei J, Yu H (2011) Effect of short-chain organic acids on the enhanced desorption of phenanthrene by rhamnolipid biosurfactant in soil–water environment. Water Res 45:5501–5510

    Article  CAS  Google Scholar 

  • Chiou CT, Peters LJ, Freed VH (1979) A physical concept of soil–water equilibria for nonionic organic compounds. Science 206:831–832

    Article  CAS  Google Scholar 

  • Chiou CT, McGroddy SE, Kile DE (1998) Partition characteristics of polycyclic aromatic hydrocarbons on soils and sediments. Environ Sci Technol 32:264–268

    Article  CAS  Google Scholar 

  • Christen K (1999) U.N. negotiations on POPs snag on malaria. Environ Sci Technol 33:444A–445A

    Article  CAS  Google Scholar 

  • Drever JI, Stillings LL (1997) The role of organic acids in mineral weathering. Colloids Surf 120:167–181

    Article  CAS  Google Scholar 

  • Folberth C, Scherb H, Suhadolc M, Munch JC, Schroll R (2009) In situ mass distribution quotient (iMDQ)—a new factor to compare bioavailability of chemicals in soils? Chemosphere 75:707–713

    Article  CAS  Google Scholar 

  • Fuhreman TM, Lichtensein EP (1978) Release of soil-bound methyl [14C] parathion residues and their uptake by earthworms and oatplants. J Agric Food Chem 26:605–611

    Article  Google Scholar 

  • Gao YZ, Shen Q, Ling WT, Ren LL (2008) Uptake of polycyclic aromatic hydrocarbons by Trifolium pretense L. from water in the presence of a nonionic surfactant. Chemosphere 72:636–643

    Article  CAS  Google Scholar 

  • Gao YZ, Ren LL, Ling WT, Gong SS, Sun BQ, Zhang Y (2010a) Desorption of phenanthrene and pyrene in soils by root exudates. Bioresour Technol 101:1159–1165

    Article  CAS  Google Scholar 

  • Gao YZ, Ren LL, Ling WT, Kang FX, Zhu XZ, Sun BQ (2010b) Effects of low-molecular-weight organic acids on sorption–desorption of phenanthrene in soils. Soil Sci Soc Am J 74:51–59

    Article  CAS  Google Scholar 

  • Gevao B, Mordaunt C, Semple KT, Piearce TG, Jones KC (2001) Bioavailability of nonextractable bound pesticide residues to earthworms. Environ Sci Technol 35:501–507

    Article  CAS  Google Scholar 

  • Gomez-Eyles JL, Collins CD, Hodson ME (2010) Relative proportions of polycyclic aromatic hydrocarbons differ between accumulation bioassays and chemical methods to predict bioavailability. Environ Pollut 158:278–284

    Article  CAS  Google Scholar 

  • Gomez-Eyles JL, Collins CD, Hodson ME (2011) Using deuterated PAH amendments to validate chemical extraction methods to predict PAH bioavailability in soils. Environ Pollut 159:918–923

    Article  CAS  Google Scholar 

  • Griffiths BS, Ritz K, Ebblewhite N, Dobson G (1999) Soil microbial community structure: effects of substrate loading rates. Soil Biol Biochem 31:145–153

    Article  CAS  Google Scholar 

  • Han AL, Yue L, Li Z, Wang HY, Wang Y, Ye QF, Lu L, Gan J (2009) Plant availability and phytotoxicity of soil bound residues of herbicide ZJ0273, a novel acetolactate synthase potential inhibitor. Chemosphere 77:955–961

    Article  CAS  Google Scholar 

  • Hatzinger PB, Alexander M (1995) Effect of aging of chemicals in soil on their biodegradability and extractability. Environ Sci Technol 29:537–545

    Article  CAS  Google Scholar 

  • Joner EJ, Corgié SC, Amellal N, Leyval C (2002) Nutritional constraints to degradation of polycyclic aromatic hydrocarbons in a simulated rhizosphere. Soil Biol Biochem 34:859–864

    Article  CAS  Google Scholar 

  • Kang FX, Chen DS, Gao YZ, Zhang Y (2010) Distribution of polycyclic aromatic hydrocarbons in subcellular root tissues of ryegrass (Lolium multiflorum Lam.). BMC Plant Biol 10:210

    Article  Google Scholar 

  • Kohl SD, Rice JA (1998) The binding of contaminants to humin: a mass balance. Chemosphere 36:251–261

    Article  CAS  Google Scholar 

  • Kong HL, Sun R, Gao YZ, Sun BQ (2013) Elution of polycyclic aromatic hydrocarbons in soil columns using low-molecular-weight organic acids. Soil Sci Soc Am J 77:72–82

    Article  CAS  Google Scholar 

  • Li H, Sheng G, Chiou CT, Xu O (2005) Relation of organic contaminant equilibrium sorption and kinetic uptake in plants. Environ Sci Technol 39:4864–4870

    Article  CAS  Google Scholar 

  • Ling WT, Ren LL, Gao YZ, Zhu XZ, Sun BQ (2009) Impact of low-molecular-weight organic acid on the availability of phenanthrene and pyrene in soil. Soil Biol Biochem 41:2187–2195

    Article  CAS  Google Scholar 

  • Ling WT, Zeng YC, Gao YZ, Dang HJ, Zhu XZ (2010) Availability of polycyclic aromatic hydrocarbons in aging soils. J Soils Sedim 10:799–807

    Article  CAS  Google Scholar 

  • Liste HH, Alexander M (2002) Butanol extraction to predict bioavailability of PAHs in soil. Chemosphere 46:1011–1017

    Article  CAS  Google Scholar 

  • Lu HL, Yan CL, Liu JC (2007) Low-molecular-weight organic acids exuded by mangrove (Kandelia candel (L.) Druce) roots and their effect on cadmium species change in the rhizosphere. Environ Exper Bot 61:159–166

    Article  CAS  Google Scholar 

  • Mckeague JA (1978) A manual of soil sampling and methods of analysis. Canadian Society of Soil Science, Ottawa

    Google Scholar 

  • Miya K, Firestone MK (2001) Enhanced phenanthrene biodegradation in soils by slender oat root exudates and root debris. J Environ Qual 30:1911–1918

    Article  CAS  Google Scholar 

  • Monteriro RTR, Queiroz BPV, Cella AC (1999) Distribution of 14C-atrazine residues in humus fractions of a Brazilian soil. Chemosphere 39:293–301

    Article  Google Scholar 

  • Mott HV (2002) Association of hydrophobic organic contaminants with soluble organic matter: evaluation of the database of K doc values. Adv Environ Res 6:577–593

    Article  CAS  Google Scholar 

  • Nakamura M, Yamada S (1996) Conditions for solid-phase extraction of agricultural chemicals in waters by using n-octanol–water partition coefficients. Analyst 121:469–475

    Article  CAS  Google Scholar 

  • Ouvrard S, Lapole D, Morel JL (2006) Root exudates impact on phenanthrene availability. Water Air Soil Pollut Focus 6:343–352

    Article  CAS  Google Scholar 

  • Palomo M, Bhandari A (2006) Impact of aging on the formation of bound residues after peroxidase-mediated treatment of 2,4-DCP contaminated soils. Environ Sci Technol 40:3402–3408

    Article  CAS  Google Scholar 

  • Puglisi E, Cappa F, Fragoulis G, Trevisan M, Del Re AA (2007) Bioavailability and degradation of phenanthrene in compost amended soils. Chemosphere 67:548–556

    Article  CAS  Google Scholar 

  • Reid BJ, Jones KC, Semple KT (2000) Bioavailability of persistent organic pollutants in soils and sediments: a perspective on mechanisms, consequences and assessment. Environ Pollut 108:103–112

    Article  CAS  Google Scholar 

  • Sabaté J, Vinas M, Solanas AM (2006) Bioavailability assessment and environmental fate of polycyclic aromatic hydrocarbons in biostimulated creosote-contaminated soil. Chemosphere 63:1648–1659

    Article  Google Scholar 

  • Saison C, Perrin-Ganier C, Amellal S, Morel JL, Schiavon M (2004) Effect of metals on the adsorption and extractability of 14C-phenanthrene in soils. Chemosphere 55:477–485

    Article  CAS  Google Scholar 

  • Semple KT, Morriss AWJ, Paton GI (2003) Bioavailability of hydrophobic organic contaminants in soils: fundamental concepts and techniques for analysis. Eur J Soil Sci 54:809–818

    Article  CAS  Google Scholar 

  • Shan J, Wang T, Li CL, Klumpp E, Ji R (2010) Bioaccumulation and bound-residue formation of a branched 4-nonylphenol isomer in the geophagous earthworm Metaphire guillelmi in a rice paddy soil. Environ Sci Technol 44:4558–4563

    Article  CAS  Google Scholar 

  • Smalla K, Wieland G, Buchner A, Zock A, Parzy J, Kaiser S, Roskot N, Heuer H, Berg G (2001) Bulk and rhizosphere soil bacterial communities studied by denaturing gradient gel electrophoresis: plant-dependent enrichment and seasonal shits revealed. Appl Environ Microbiol 67:4742–4751

    Article  CAS  Google Scholar 

  • Sun HW, Yan QS (2008) Influence of pyrene combination state in soils on its treatment efficiency by Fenton oxidation. J Environ Manag 88:556–563

    Article  CAS  Google Scholar 

  • Sun TR, Cang L, Wang QY, Zhou DM (2010) Roles of abiotic losses, microbes, plant roots, and root exudates on phytoremediation of PAHs in a barren soil. J Hazard Mater 176:919–925

    Article  CAS  Google Scholar 

  • Sun BQ, Gao YZ, Liu J, Sun YD (2012) Impact of different root exudates constituents on the availability of phenanthrene in soil. Soil Sci Soc Am 76:2041–2050

    Article  CAS  Google Scholar 

  • Wania F, Mackay D (1996) Tracking the distribution of persistent organic pollutants. Environ Sci Technol 30:390A–396A

    Article  CAS  Google Scholar 

  • Weber WJJ, Kim SH, Johnson MD (2002) Distributed reactivity model for sorption by soils and sediments: 15 high-concentration co-contaminant effects on phenanthrene sorption and desorption. Environ Sci Technol 36:3625–3634

    Article  CAS  Google Scholar 

  • White JC, Mattina MI, Lee WY, Eitzer BD, Iannucci-Berger W (2003) Role of organic acids in enhancing the desorption and uptake of weathered p, p′-DDE by Cucurbita pepo. Environ Pollut 124:71–80

    Article  CAS  Google Scholar 

  • Yang Y, Ratté D, Smets BF, Pignatello JJ, Grasso D (2001) Mobilization of soil organic matter by complexing agents and implications for polycyclic aromatic hydrocarbon desorption. Chemosphere 43:1013–1021

    Article  CAS  Google Scholar 

  • Yaws CL (1999) Chemical properties handbook. McGraw-Hill, New York

    Google Scholar 

  • Yoshitomi KJ, Shann JR (2001) Corn (Zea mays L.) root exudates and their impact on 14C-pyrene mineralization. Soil Biol Biochem 33:1769–1776

    Article  CAS  Google Scholar 

  • Zhao Q, Li PJ, Stagnitti F, Ye J, Dong DB, Zhang YQ, Li P (2009) Effects of aging and freeze-thawing on extractability of pyrene in soil. Chemosphere 74:447–452

    Article  Google Scholar 

  • Zhu YH, Zhang SZ, Huang HL, Wei B (2009) Effects of maize root exudates and organic acids on the desorption of phenanthrene from soils. J Environ Sci 21:920–926

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by the National Natural Science Foundation of China (21077056, 51278252, 41171380, and 41171193), the Fundamental Research Funds for the Central Universities of China (KYZ201109), and the Key Technology R&D Program of Jiangsu Province (BE2011780).

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Authors and Affiliations

  1. Institute of Organic Contaminant Control and Soil Remediation, College of Resource and Environmental Sciences, Nanjing Agricultural University, Nanjing, 210095, People’s Republic of China

    Bingqing Sun, Wanting Ling & Yize Wang

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  1. Bingqing Sun
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  2. Wanting Ling
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  3. Yize Wang
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Correspondence to Wanting Ling.

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Responsible editor: Jean-Paul Schwitzguébel

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Sun, B., Ling, W. & Wang, Y. Can root exudate components influence the availability of pyrene in soil?. J Soils Sediments 13, 1161–1169 (2013). https://doi.org/10.1007/s11368-013-0712-4

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  • DOI: https://doi.org/10.1007/s11368-013-0712-4

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