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Kocuria rosea HN01, a newly alkaliphilic humus-reducing bacterium isolated from cassava dreg compost

  • IHSS 16: FUNCTIONS OF NATURAL ORGANIC MATTER IN CHANGING ENVIRONMENT
  • Published:
Journal of Soils and Sediments Aims and scope Submit manuscript

Abstract

Purpose

The aims of this study were to isolate an alkaliphilic humus-reducing bacterium, investigate the fastest microbial reduction of humus analog as affected by different cultivation, and examine its ability for iron(III) oxide reduction and organochlorine pollutants (OCPs) degradation.

Materials and methods

A strain of pure culture, designated as HN01, was isolated from cassava dreg compost using anaerobic enrichment procedure with glucose as the electron donor and anthraquinone-2,6-disulphonate (AQDS) as the sole terminal electron acceptor. The isolate strain was identified using phenotypic and phylogenetic analysis. Iron(III) oxides and OCPs were chosen as potential electron acceptors. Strict anaerobic techniques and sterile conditions were applied throughout the incubation experiments, purged with O2-free N2 for 15 min. The concentration of reduced AQDS and Fe(II) was then quantified using a UV–vis spectrophotometer. The concentration of OCPs was analyzed by gas chromatography with a micro-electron capture detector. Cell number was determined by direct plate counting on aerobic Luria–Bertani medium agar medium at pH 9.

Results and discussion

(1) Strain HN01 was identified as Kocuria rosea, and the AQDS reduction by HN01 was observed in NaCl concentrations below 12 % (w/v) (optimum, 10 %) and pH ranges of 6.0–10.0 (optimum, 9.0) with sucrose as electron donor at 30 °C; (2) glucose, sucrose, methanol, ethanol, glycerol, and acetate were the favorable electron donors for AQDS reduction by strain HN01; (3) the strain had the ability of reducing iron(III) oxides in the presence of sucrose at pH 9.0 and its Fe(III)-reducing capacity ranked as goethite (α-FeOOH) > lepidocrocite (γ-FeOOH) > haematite(α-Fe2O3); and (4) the strain could effectively dechlorinate p,p′-DDT (1,1,1-trichloro-2,2-bis(4-chlorophenyl) ethane), and the dechlorination rate reached 71.3 %.

Conclusions

This is the first report of a strain of K. rosea capable of reducing AQDS, iron (III) oxides, and p,p′-DDT, which extends the diversity of the alkaliphilic and halotolerant humus/Fe(III)-reducing bacterium associated with dechlorination. The strain may have the potential to be used for bioremediation of an anoxic alkaline wastewater or site contaminated with OCPs.

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Acknowledgments

The authors thank the National Natural Science Foundations of China (no. 41101477, 21007091) and the Fundamental Research Funds for Environment and Plant Protection Institute, CATAS (no. 2011Hzs1J006, 2010hzsZDZX001) for financial support for this study.

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Authors and Affiliations

  1. Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, People’s Republic of China

    Chun-Yuan Wu, Nan Chen, Hong Li & Qin-Fen Li

  2. College of Environment and Plant Protection, Hainan University, Haikou, 570228, People’s Republic of China

    Nan Chen

  3. Danzhou Scientific Observing and Experimental Station of Agro-Environment, Ministry of Agriculture, Danzhou, 571737, People’s Republic of China

    Hong Li

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  1. Chun-Yuan Wu
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  2. Nan Chen
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  3. Hong Li
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Correspondence to Qin-Fen Li.

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Responsible editor: Jizheng He

Chun-Yuan Wu and Nan Chen contributed equally to this work.

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Wu, CY., Chen, N., Li, H. et al. Kocuria rosea HN01, a newly alkaliphilic humus-reducing bacterium isolated from cassava dreg compost. J Soils Sediments 14, 423–431 (2014). https://doi.org/10.1007/s11368-013-0679-1

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  • DOI: https://doi.org/10.1007/s11368-013-0679-1

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