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Impacts of long-term inorganic and organic fertilization on lignin in a Mollisol

  • SOILS, SEC 1 • SOIL ORGANIC MATTER DYNAMICS AND NUTRIENT CYCLING • RESEARCH ARTICLE
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Abstract

Purpose

Fertilization is an essential management method to maintain and increase soil organic carbon (SOC) content in agroecosystems. Fertilizer application is known to markedly influence the turnover of labile and refractory SOC components. However, the dynamics of lignin in response to different types of fertilization remains unclear. This study addresses the impact of long-term fertilization on lignin accumulation in an arable soil.

Materials and methods

Samples from a Mollisol soil were collected from 0 to 20 and 20 to 40 cm below the ground surface at a long-term experimental site with maize (Zea mays L.) monoculture. Experimental plots received one of six fertilization options: nitrogen (N) only; N and phosphorus (P); N, P, and potassium (K); pig manure at either of two application rates; or no fertilizer. Lignin levels were quantified by alkaline CuO oxidation of the samples to release lignin monomers, followed by gas chromatography. Lignin-phenol content was calculated as the sum of vanillyl (V), syringyl (S), and cinnamyl (C) type phenols. In addition, the acid-to-aldehyde ratios of V- and S-type monomers were used to assess the degree of lignin degradation.

Results and discussion

After a 29-year test period, inorganic fertilizer applications exhibited no significant effect on soil lignin content or the proportion of lignin-derived carbon in SOC for the 0–20-cm layer. However, inorganic fertilization markedly increased both measures in the 20–40-cm layer because of the high lignin content of crop residue as well as low microbial decomposition of lignin. In contrast, long-term organic fertilization resulted in significant lignin increases in bulk soil and SOC in both layers. This was mainly attributed to the higher lignin content of the organic fertilizer (pig manure). Lignin degradation in the 0–20-cm layer was evidently retarded after fertilization, while neither inorganic nor organic fertilizers exhibited any influence on the degree of oxidation of lignin phenols in the 20–40-cm layer.

Conclusions

Selective accumulation of lignin was enhanced after long-term organic fertilization, which was coincident with SOC sequestration. The results suggest that the long-term organic fertilization facilitates lignin stabilization in both soil layers, while the long-term application of inorganic fertilizer can only be beneficial to lignin accumulation in subsoil (20–40-cm layer) of the Mollisol. This study elucidates effects of long-term fertilization on lignin dynamics and has significant implications for sustainable management of Mollisols under the maize cropping system in northeastern China.

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References

  • Allen SE, Grimshaw HM, Rowland AP (1986) Chemical analysis. Blackwell Scientific, Oxford, pp 285–344

    Google Scholar 

  • Amelung W, Flach KW, Zech W (1999) Lignin in particle-size fractions of native grassland soils as influenced by climate. Soil Sci Soc Am J 63:1222–1228

    Article  CAS  Google Scholar 

  • Bahri H, Dignac MF, Rumpel C, Rasse DP, Chenu C, Mariotti A (2006) Lignin turnover kinetics in an agricultural soil is monomer specific. Soil Biol Biochem 38:1977–1988

    Article  CAS  Google Scholar 

  • Bahri H, Rasse DP, Rumpel C, Dignac MF, Bardoux G, Mariotti A (2008) Lignin degradation during a laboratory incubation followed by 13C isotope analysis. Soil Biol Biochem 40:1916–1922

    Article  CAS  Google Scholar 

  • Bittman S, Forge TA, Kowalenko CG (2005) Responses of the bacterial and fungal biomass in a grassland soil to multi-year applications of diary manure slurry and fertilizer. Soil Biol Biochem 37:613–623

    Article  CAS  Google Scholar 

  • Blair N, Faulkner RD, Till AR, Poulton PR (2006) Long-term management impacts on soil C, N and physical fertility part I: broadbalk experiment. Soil Tillage Res 91:30–38

    Article  Google Scholar 

  • Derenne S, Largeau C (2001) A review of some important families of refractory macromolecules: composition, origin, and fate in soils and sediments. Soil Sci 166:833–847

    Article  CAS  Google Scholar 

  • Dignac MF, Bahr H, Rumpe C, Rasse DP, Bardoux G, Balesdent J, Girardin C, Chenu C, Mariotti A (2005) Carbon-13 natural abundance as a tool to study the dynamics of lignin monomers in soil: an appraisal at the Closequx experimental field (France). Geoderma 128:3–17

    Article  CAS  Google Scholar 

  • Donaldson LA (2001) Lignification and lignin topochemistry—an untrastructural view. Phytochem 57:859–873

    Article  CAS  Google Scholar 

  • Flessa H, Amelung W, Helfrich M, Wiesenberg GLB, Gleixner G, Brodowski S, Rethemeyer J, Kramer C, Grootes PM (2008) Storage and stability of organic matter and fossil carbon in a Luvisol and Phaeozem with continuous maize cropping: a synthesis. J Plant Nutr Soil Sci 171:36–51

    Article  CAS  Google Scholar 

  • Ge GF, Li ZJ, Fan FL, Chu GX, Hou ZA, Liang YC (2010) Soil biological activity and their seasonal variations in response to long-term application of organic and inorganic fertilizers. Plant Soil 326:31–44

    Article  CAS  Google Scholar 

  • Gong W, Yan XY, Wang JY, Hu TX, Gong YB (2009) Long-term manure and fertilizer effects on soil organic matter fractions and microbes under a wheat-maize cropping system in northern China. Geoderma 149:318–324

    Article  CAS  Google Scholar 

  • Guggenberger G, Ghristensen BT, Zech W (1994) Land-use effects on the composition of organic matter in particle-size separates of soil: I. lignin and carbohydrate signature. Eur J Soil Sci 45:449–458

    Article  CAS  Google Scholar 

  • Guggenberger G, Zech W, Thomas RJ (1995) Lignin and carbohydrate alteration in particle-size separates of an oxisol under tropical pastures following native savanna. Soil Biol Biochem 27:1629–1638

    Article  CAS  Google Scholar 

  • Haider K (1992) Problems related to the humification processes in soils of temperate climates. In: Stotzky G, Bollag JM (eds) Soil biochemistry. Marcel Dekker, New York, pp 55–94

    Google Scholar 

  • Hautala K, Peuravuori J, Pihlaja K (1997) Estimation of origin of lignin in humic DOM by CuO-oxidation. Chemosphere 35:809–817

    Article  CAS  Google Scholar 

  • Hati KM, Swarup A, Mishra B, Manna MC, Wanjari RH, Mandal KG, Misra AK (2008) Impact of long-term application of fertilizer, manure and lime under intensive cropping on physical properties and organic carbo content of an Alfisol. Geoderma 148:173–179

    Article  CAS  Google Scholar 

  • Hedges JI, Ertel JR (1982) Characterization of lignin by gas capillary chromatography of cupric oxide oxidation products. Anal Chem 54:174–178

    Article  CAS  Google Scholar 

  • Heim A, Schmidt MWI (2007a) Lignin is preserved in the fine silt fraction of an arable Luvisol. Org Geochem 38:2001–2011

    Article  CAS  Google Scholar 

  • Heim A, Schmidt MWI (2007b) Lignin turnover in arable soil and grassland analysed with two different labelling approaches. Eur J Soil Sci 58:599–608

    Article  CAS  Google Scholar 

  • Henriksen TM, Breland TA (1999) Nitrogen availability effects on carbon mineralization, fungal and bacterial growth, and enzyme activities during decomposition of wheat straw in soil. Soil Biol Biochem 31:1121–1134

    Article  CAS  Google Scholar 

  • Hofmann A, Heim A, Gioacchini P, Miltner A, Gehre M, Schmidt MWI (2009) Mineral fertilization did not affect decay of old lignin and SOC in a 13C-labeled arable soil over 36 years. Biogeosci 6:1139–1148

    Article  CAS  Google Scholar 

  • Hofrichter M, Vares K, Scheibner K, Galkin S, Sipila J, Hatakka A (1999) Mineralization and solubilization of synthetic lignin by manganese peroxidases from Nematoloma frowardii and Phlebia radiata. J Biotechnol 67:217–228

    Article  CAS  Google Scholar 

  • Hofrichter M (2002) Review: lignin conversion by manganese peroxidase (MnP). Enzym Microb Technol 30:454–466

    Article  CAS  Google Scholar 

  • Hopkins DW, Shiel RS (1996) Size and activity of soil microbial communities in long-term experimental grassland plots treated with manure and inorganic fertilizers. Biol Fertil Soils 22:66–70

    Article  Google Scholar 

  • Huggins DR, Buyanovsky GA, Wagner GH, Brown JR, Darmody RG, Peck TR, Lesoing GW, Vanotti MB, Bundy LG (1998) Soil organic C in the tallgrass prairie-derived region of the corn belt: effects of long-term crop management. Soil Tillage Res 47:219–234

    Article  Google Scholar 

  • Jarecki MK, Lal R (2003) Crop management for soil carbon sequestration. Criti Reviews Plant Sci 22:471–502

    Article  Google Scholar 

  • Kögel I (1986) Estimation and decomposition pattern of the lignin component in forest humus layers. Soil Biol Biochem 18:589–594

    Article  Google Scholar 

  • Kögel I, Bochter R (1985) Characterization of lignin in forest humus layers by high-performance liquid chromatography of cupric oxide oxidation products. Soil Biol Biochem 17:637–640

    Article  Google Scholar 

  • Kögel I, Hempfling R, Zech W, Hatcher PG, Schulten HR (1988) Chemical-composition of the organic-matter in forest soils. 1. Forest litter. Soil Sci 146:124–136

    Article  Google Scholar 

  • Kandeler E, Stemmer M, Klimanek EM (1999) Response of soil microbial biomass, urease and xylanase within particle size fractions to long-term soil management. Soil Biol Biochem 31:261–273

    Article  CAS  Google Scholar 

  • Keeler BL, Hobbie SE, Kellogg LE (2009) Effects of long-term nitrogen addition on microbial enzyme activity in eight forested and grassland sites: implications for litter and soil organic matter decomposition. Ecosys 12:1–15

    Article  CAS  Google Scholar 

  • Kiem R, Kögel-Knabner I (2003) Contribution of lignin and polysaccharides to the refractory carbon pool in C-depleted arable soils. Soil Biol Biochem 35:101–118

    Article  CAS  Google Scholar 

  • Killops S, Killops V (2005) Introduction to organic geochemistry, 2nd edn. Geofluids 5:236–237

    Article  Google Scholar 

  • Kirk TK, Chang HM, Lorenz LF (1975) Topochemistry of fungal degradation of lignin in birch wood as related to distribution of guaiacyl and syringyl lignins. Wood Sci Technol 9:81–86

    Article  CAS  Google Scholar 

  • Kirk TK, Connors WJ, Zeikus JG (1976) Requirement for a growth substrate during lignin decomposition by 2 wood-rotting fungi. Appl Environ Microbiol 32:192–194

    CAS  Google Scholar 

  • Ladd JN, Amato M, Zhou LK, Schultz JE (1994) Differential-effects of rotation, plant residue and nitrogen-fertilizer on microbial biomass and organic-matter in an Australian Alfisoil. Soil Biol Biochem 26:821–831

    Article  Google Scholar 

  • Leonowicz A, Matuszewska A, Luterek J, Ziegenhagen D, Wojtas-Wasilewska M, Cho NS, Hofrichter M, Rogalski J (1999) Biodegradation of lignin by white rot fungi. Fungal Genet Biol 27:175–185

    Article  CAS  Google Scholar 

  • Lobe I, Du Preez CC, Amelung W (2002) Influence of prolonged arable cropping on lignin compounds in sandy soils of the South African Highveld. Eur J Soil Sci 53:553–562

    Article  CAS  Google Scholar 

  • Lundell T, Leonowicz A, Rogalski J, Hatakka A (1990) Formation and action of lignin-modifying enzymes in cultures of Phlebia-radiata supplemented with veratric acid. Appl Environ Microbiol 56:2623–2629

    CAS  Google Scholar 

  • Molloy LF, Bridger BA, Cairns A (1977) Studies on a climosequence of soils in tussock grasslands.13. Structural carbohydrates in tussock leaves, roots, and litter and in soil light and heavy fractions. N-Z J Sci 20:443–451

    CAS  Google Scholar 

  • Mondini C, Cayuela ML, Sanchez-Monedero MA, Roig A, Brookes PC (2006) Soil microbial biomass activation by trace amounts of readily available substrate. Biol Fertil Soils 42:542–549

    Article  Google Scholar 

  • Paul EA, Clark FE (1996) Soil microbiology and biochemistry, 2nd edn. Academic, San Diego

    Google Scholar 

  • Plaza C, Hernandez D, Garcia-Gil JC, Polo A (2004) Microbial activity in pig slurry-amended soils under semiarid conditions. Soil Biol Biochem 36:1577–1585

    Article  CAS  Google Scholar 

  • Purakayastha TJ, Rudrappa L, Singh D, Swarup A, Bhadraray S (2008) Long-term impact of fertilizers on soil organic carbon pools and sequestration rates in maize-wheat-cowpea cropping system. Geoderma 144:370–378

    Article  CAS  Google Scholar 

  • Ralph J, Helm RF, Quideau S, Hatfield RD (1992) Lignin feruloyl ester cross-links in grasses.1. Incorporation of feruloyl esters into coniferyl alcohol dehydrogenation polymers. J chem Soc Trans 1:2961–2969

    Google Scholar 

  • Rasse DP, Dignac MF, Bahri H, Rumpel C, Mariotti A, Chenu C (2006) Lignin turnover in an agricultural field: from plant residues to soil-protected fractions. Eur J Soil Sci 57:530–538

    Article  CAS  Google Scholar 

  • Rudrappa L, Purakayastha TJ, Singh D, Bhadraray S (2006) Long-term manuring and fertilization effects on soil organic carbon pools in a Typic Haplustept of semi-arid sub-tropical India. Soil Tillage Res 88:180–192

    Article  Google Scholar 

  • Sarathchandra SU, Ghani A, Yeates GW, Burch G, Cox NR (2001) Effect of nitrogen and phosphate fertilisers on microbial and nematode diversity in pasture soils. Soil Biol Biochem 33:953–964

    Article  CAS  Google Scholar 

  • Scalbert A, Monties B, Lallemand JY, Guittet E, Rolando C (1985) Ether linkage between phenolic-acids and lignin fractions from wheat straw. Phytochem 24:1359–1362

    Article  CAS  Google Scholar 

  • Sinsabaugh RL, Lauber CL, Weintraub MN, Ahmed B, Allison SD, Crenshaw C, Contosta AR, Cusack D, Frey S, Gallo ME, Gartner TB, Hobbie SE, Holland K, Keeler BL, Powers JS, Stursova M, Takacs-Vesbach C, Waldrop MP, Wallenstein MD, Zak DR, Zeglin LH (2008) Stoichiometry of soil enzyme activity at global scale. Ecol Lett 11:1252–1264

    Google Scholar 

  • Sjöberg G, Knicker H, Nilsson SI, Berggren D (2004) Impact of long-term N fertilization on the structural composition of spruce litter and mor humus. Soil Biol Biochem 36:609–618

    Article  Google Scholar 

  • Solomon D, Lehmann J, Zech W (2000) Land use effects on soil organic matter properties of chromic luvisols in semi-arid northern Tanzania: carbon nitrogen, lignin and carbohydrates. Agric Ecosyst Environ 78:203–213

    Article  CAS  Google Scholar 

  • Soil Survey Staff (2003) Keys to soil taxonomy, 9th edn. United States Department of Agriculture and Natural Resources Conservation Service, Washington

    Google Scholar 

  • Suhas Carrott PJM, Carrott MMLR (2007) Lignin—from natural adsorbent to activated carbon: a review. Bioresour Technol 98:2301–2312

    Article  Google Scholar 

  • Yan DZ, Wang DJ, Yang LZ (2007) Long-term effect of chemical fertilizer, straw, and manure on labile organic matter fractions in a paddy soil. Biol Fertil Soils 44:93–101

    Article  Google Scholar 

  • Yang XM, Zhang XP, Fang HJ, Zhu P, Ren J, Wang LC (2003) Long-term effects of fertilization on soil organic carbon changes in continuous corn of northeast China: RothC model Simulations. Environ Manage 32:459–465

    Article  CAS  Google Scholar 

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Acknowledgements

This research was supported by a grant from the Major State Basic Research Development Program of China (973 Program) (No. 2009CB118607) and the International Partnership Program of Chinese Academy of Sciences (No. KZCX2-YW-T06). We are grateful to Dr. Denise Parker for her English editing and two anonymous reviewers for helpful comments to improve the manuscript.

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Authors and Affiliations

  1. Key Laboratory of Terrestrial Ecological Process, Institute of Applied Ecology, Chinese Academy of Sciences, Wenhua Road 72, Shenyang, 110016, People’s Republic of China

    Ning Liu, Hongbo He, Hongtu Xie, Zhen Bai & Xudong Zhang

  2. Graduate School of Chinese Academy of Sciences, Beijing, 100049, People’s Republic of China

    Ning Liu

  3. Jilin Academy of Agricultural Sciences, Gongzhuling, 136100, People’s Republic of China

    Chang Peng, Ping Zhu, Jun Ren & Lichun Wang

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  1. Ning Liu
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  2. Hongbo He
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Correspondence to Xudong Zhang.

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Liu, N., He, H., Xie, H. et al. Impacts of long-term inorganic and organic fertilization on lignin in a Mollisol. J Soils Sediments 10, 1466–1474 (2010). https://doi.org/10.1007/s11368-010-0298-z

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  • DOI: https://doi.org/10.1007/s11368-010-0298-z

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