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Postprandial oxidative stress is modulated by dietary fat in adipose tissue from elderly people

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Abstract

We have investigated whether dietary fat modifies the postprandial oxidative stress in adipose tissue of elderly people. Twenty participants received three diets for 4 weeks each: SFA-rich diet, Mediterranean (Med) diet enriched in MUFA with virgin olive oil, and a low-fat, high-carbohydrate diet enriched in n-3 PUFA (α-linolenic acid from plant origin) (CHO-PUFA diet). After 12 h of fasting, volunteers received a breakfast reflecting the fatty acid composition of the diet ingested in the preceding dietary period. Med diet induced higher postprandial SOD2 and TrxR mRNA levels, and CHO-PUFA diet induced higher GPx1 and TrxR mRNA levels compared with SFA-rich diet. Med and CHO-PUFA breakfasts induced a postprandial increase in plasma reduced glutathione (GSH), and a greater postprandial GSH/oxidized glutathione ratio compared to the SFA-rich diet. Our study suggests that the consumption of Med and CHO-PUFA diets may reduce postprandial oxidative stress compared to an SFA-rich diet, which may be due to higher antioxidant enzymes gene expression in adipose tissue.

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Acknowledgments

The CIBEROBN is an initiative of the Instituto de Salud Carlos III, Madrid, Spain. We thank Maria Jose Gomez-Luna for technical support. This study was supported in part by research grants from the Spanish Ministry of Science and Innovation (AGL2004-07907, AGL2006-01979, and AGL2009-12270 to J L-M; SAF07-62005 and PI10/02412 to F P-J; and FIS PI10/01041 to P P-M); Consejería de Economía, Innovación y Ciencia, Proyectos de Investigación de Excelencia, Junta de Andalucía (P06-CTS-01425 to J L-M; CTS5015 and AGR922 to F P-J); Consejería de Salud, Junta de Andalucía (06/128, 07/43, and PI0193/09 to J L-M; 06/129 to F P-J; 0118/08 to F F-J; PI-0252/09 to J D-L; and PI-0058/10 to P P-M); Fondo Europeo de Desarrollo Regional.

Conflict of interest

None of the authors has any conflict of interests that could affect the performance of the work or the interpretation of the data.

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Authors and Affiliations

  1. Lipids and Atherosclerosis Research Unit, IMIBIC/Reina Sofía University Hospital/University of Córdoba, Avda. Menéndez Pidal, s/n, 14004, Córdoba, Spain

    Eliana Romina Meza-Miranda, Antonio Camargo, Oriol Alberto Rangel-Zuñiga, Javier Delgado-Lista, Antonio Garcia-Rios, Pablo Perez-Martinez, Francisco Perez-Jimenez & José Lopez-Miranda

  2. CIBER Fisiopatología de la Obesidad y la Nutrición (CIBEROBN), Instituto de Salud Carlos III, Madrid, Spain

    Eliana Romina Meza-Miranda, Antonio Camargo, Oriol Alberto Rangel-Zuñiga, Javier Delgado-Lista, Antonio Garcia-Rios, Pablo Perez-Martinez, Francisco J. Tinahones, Francisco Perez-Jimenez & José Lopez-Miranda

  3. Department of Biochemistry and Molecular Biology, Faculty of Medicine, IMIBIC/Hospital Universitario Reina Sofıa/Universidad de Cordoba, 14004, Cordoba, Spain

    Inma Tasset-Cuevas & Isaac Tunez

  4. Hospital Virgen de la Victoria, Málaga, Spain

    Francisco J. Tinahones

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  1. Eliana Romina Meza-Miranda
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  2. Antonio Camargo
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  4. Javier Delgado-Lista
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  7. Inma Tasset-Cuevas
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  10. Francisco Perez-Jimenez
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  11. José Lopez-Miranda
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Corresponding author

Correspondence to José Lopez-Miranda.

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Eliana Romina Meza-Miranda and Antonio Camargo equally contributed to this work.

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Meza-Miranda, E.R., Camargo, A., Rangel-Zuñiga, O.A. et al. Postprandial oxidative stress is modulated by dietary fat in adipose tissue from elderly people. AGE 36, 507–517 (2014). https://doi.org/10.1007/s11357-013-9579-y

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